Cell and Tissue Research

, Volume 149, Issue 3, pp 349–361 | Cite as

Centrioles and cilia multiplication in the pituitary of the rat after furosemid and colchicine treatment

I. The posterior lobe
  • J.-P. Hubert
  • J. Flament-Durand
  • P. Dustin


In adult rats, dehydration produced by the injection of Furosemid (Lasix®) induces the formation of new centrioles in the endothelial cells of the posterior lobe of the hypophysis.

Colchicine considerably amplifies this phenomenon and centriologenesis and cilia formation are strikingly apparent in the pituicytes. In control animals, centrioles are quite exceptional in pituicytes and endothelial cells. All steps of the assembly of centrioles from a granular matrix, and the differentiation of atypical (mainly 9+0 and 8+1) cilia are observed in pituicytes. Dehydration increases the mitotic activity of the pituicytes. The respective roles of dehydration, Furosemid and colchicine in the stimulation of centriologenesis are briefly discussed.

This work was supported by a grant (1973–1974) from the Belgian National Fund for Scientific Research and by grant no. 1.120 from the Belgian National Fund for Medical Research.

Key words

Pituicytes Endothelial cells Centrioles Cilia formation Influence of Furosemid (dehydration) and colchicine 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Allen, R. A.: Isolated cilia in inner retinal neurons and in retinal pigment epithelium. J. Ultrastruct. Res. 12, 730–747 (1965)Google Scholar
  2. Banerjee, S., Kerr, V., Winston, M., Kelleher, J. K., Margulis, L.: Melatonin: Inhibition of microtubule-based oral morphogenesis in Stentor coeruleus. J. Protozool. 19, 108–112 (1972)Google Scholar
  3. Barnes, B. G.: Ciliated secretory cells in the pars distalis of the mouse hypophysis. J. Ultrastruct. Res. 5, 453–467 (1961)Google Scholar
  4. Benda, C.: Hypophysis cerebri. In: M. von Hirsch (edit.), Handbuch der inneren Sekretion, vol. 2, p. 867–909. Leipzig: Kabitzch (1932)Google Scholar
  5. Boudier, J. A., Detieux, Y., Dutillet, B., Cau, P.: Effets d'injections intracisternales de colchicine sur la neurohypophyse du rat déshydraté après privation d'eau. C. R. Acad. Sci. (Paris) 165, 1089–1092 (1971)Google Scholar
  6. Dahl, H. A.: Fine structure of cilia in rat cerebral cortex. Z. Zellforsch. 60, 369–386 (1963)Google Scholar
  7. Dahl, H. A.: On the cilium cell relationship in the adenohypophysis of the mouse. Z. Zellforsch. 83, 169–177 (1967)Google Scholar
  8. Desclin, L.: A propos des réactions morphologiques du lobe postérieur de l'hypophyse au cours des états de déshydratation chez le rat blanc. C. R. Soc. Biol. (Paris) 141, 438–439 (1947)Google Scholar
  9. Dubois, P., Girod, C.: Les cellules ciliées de l'antéhypophyse. Etude au microscope électronique Z. Zellforsch. 103, 502–507 (1970)Google Scholar
  10. Dustin, P.: Some recent advances in the study of microtubules and microtubule poisons. Arch. Biol. (in press)Google Scholar
  11. Erkoçak, A.: Etude au microscope électronique de la neurohypophyse du rat normal ou après surrénalectomie bilatérale. Recherches sur les pituicytes et sur le mode de libération du neurosecrétat. Acta anat. (Basel) 84, 178–201 (1973)Google Scholar
  12. Fauré-Frémiet, E.: Cils vibratiles et flagelles. Biol. Rev. 36, 464–536 (1961)Google Scholar
  13. Fauré-Frémiet, E.: Morphogénèse et physiologie de la ciliation. Path. et Biol. (Basel) 9, 811–816 (1961)Google Scholar
  14. Fawcett, D. W.: Cilia and flagella. In: J. Brachet, A. E. Mirsky (edit.), The cell, vol. 2, p. 217–297. New York-London: Academic Press (1961)Google Scholar
  15. Feit, P. N., Bruun, H., Nielsen, C. K.: Aminobenzoic acid diuretics. 1. 4-Halogeno-5-sulfamylmetanilic acid derivatives. J. med. Chem. 13, 1071–1075 (1970)Google Scholar
  16. Fernandez, H. L., Burton, P. R., Samson, F. E.: Axoplasmic transport in the crayfish nerve cord. The role of fibrillar constituents of neurons. J. Cell Biol. 51, 176–192 (1971)Google Scholar
  17. Flament-Durand, J., Dustin, P.: Studies on the transport of secretory granules in the magnocellular hypothalamic neurons. I. Action of colchicine on axonal flow and neurotubules in the paraventricular nuclei. Z. Zellforsch. 130, 440–454 (1972)Google Scholar
  18. Gersh, I.: Structure and function of parenchymatous glandular cells in neurohypophysis of the rat. Amer. J. Anat. 64, 407–443 (1939)Google Scholar
  19. Hansson, H. A., Norström, A.: Glial reactions induced by colchicine-treatment of the hypothalamic -neurohypophyseal system. Z. Zellforsch. 113, 294–310 (1971)Google Scholar
  20. Harris, G. W., Donovan, B. T.: The pituitary gland, vol. 3: Pars intermedia and neurohypophysis, p. 210–212. London: Butterworths (1966)Google Scholar
  21. Krsulovic, J., Brückner, G.: Morphological characteristics of pituicytes in different functional stages. Light and electron microscopy of the neurohypophysis of the albino rat. Z. Zellforsch. 99, 210–220 (1969)Google Scholar
  22. Krsulovic, J., Ermisch, A., Sterba, G.: Electron microscopic and autoradiographic study on the neurosecretory system of albino rats with special considerations of the pituicyte problem. In: W. Bargmann, B. Scharrer (edit.), Aspects of neuroendocrinology, p. 166–172. Berlin-Heidelberg-New York: Springer 1970Google Scholar
  23. Lallier, R.: Biochemical aspects of animalization and vegetalization in the sea-urchin embryo. In: M. Abercrombie, J. Brachet (edit.), Advances in morphogenesis, vol. 3, p. 147–196. New York-London: Academic Press 1964Google Scholar
  24. Leeson, T. S.: Electron microscopy of the rete testis of the rat. Anat. Rec. 144, 57–67 (1962)Google Scholar
  25. Leveque, T. P., Small, M.: The relationship of the pituicyte to the posterior lobe hormones. Endocrinology 65, 909–915 (1959)Google Scholar
  26. Meyer, J., Bencosme, S. A.: The fine structure of normal rabbit pancreatic islet cells. Rev. canad. Biol. 24, 179–205 (1965)Google Scholar
  27. Milhaud, M., Pappas, G. D.: Cilia formation in the adult cat brain after pargyline treatment. J. Cell Biol. 37, 599–609 (1968)Google Scholar
  28. Murray, M.: Effects of dehydration on the rate of proliferation of hypothalamic neuroglial cells. Exp. Neurol. 20, 460–468 (1968)Google Scholar
  29. Ortmann, R.: Über experimentelle Veränderungen der Morphologie der Hypophysen—Zwischenhirnsysteme und die Beziehung der sog. “Gomorisubstanz” zum Adiuretin. Z. Zellforsch. 36, 92–140 (1951)Google Scholar
  30. Rennel, E. G., Drager, G. A.: The relationship of pituicytes to neurosecretion. Anat. Rec. 122, 193–200 (1955)Google Scholar
  31. Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 7, 280–313 (1963)Google Scholar
  32. Rosenbaum, J., Carlson, A.: Cilia regeneration in Tetrahymena and inhibition by colchicine. J. Cell Biol. 40, 415–425 (1969)Google Scholar
  33. Sachs, H., Saito, S., Sunde, D.: Biochemical studies on the neurosecretory and neuroglial cells of the hypothalamo-neurohypophysial complex. In: H. Heller, K. Lederis (edit.), Subcellular organization and function in endocrine tissue, p. 325–336. Cambridge: University Press 1971Google Scholar
  34. Selye, H., Hall, C. E.: Further studies concerning the action of sodium chloride on the pituitary. Anat. Rec. 86, 579–583 (1943)Google Scholar
  35. Sorokin, S. P.: Reconstructions of centriole formation and ciliogenesis in mammalian lungs. J. Cell Sci. 3, 207–230 (1968)Google Scholar
  36. Sterba, G., Brückner, G.: Elektronenmikroskopische Untersuchungen über die Reaktion der Pituizyten nach Hypophysenstieldurchtrennung bei Rana esculenta. Z. Zellforsch. 94, 425–433 (1969)Google Scholar
  37. Stubblefield, E., Brinkley, B. R.: Cilia formation in Chinese hamster fibroblasts in vitro as a response to colcemid treatment. J. Cell Biol. 30, 645–652 (1966)Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • J.-P. Hubert
    • 1
  • J. Flament-Durand
    • 1
  • P. Dustin
    • 1
  1. 1.Departments of Pathology and Electron MicroscopyUniversité Libre de BruxellesBelgium

Personalised recommendations