Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

The effects of glycerol treatment on the isolated rat heart

  • 19 Accesses

  • 1 Citations

Summary

Isolated rat hearts were perfused with a balanced electrolyte solution containing 1000mM glycerol for 15min and then perfused with normal electrolyte solution for up to 32 min. The perfusion with hypertonic glycerol solution and subsequent washout is termed “glycerol treatment”. Initially, glycerol removal causes swelling and rupture of the T-system in ventricular myocardial cells which correlates temporally with a period of cardiac arrest. Contractility returns during further glycerol removal and concomitant recovery of the T-system is observed. Atomic absorption spectometry and neutron activation analysis were used to measure ventricular sodium, potassium and calcium ion content. There is no apparent correlation between changes in ion content and cardiac arrest or recovery. The water movements were calculated from wet weight, dry weight and inulin space, and confirmed by morphometric analysis of extracellular and intracellular space. It is suggested that the swelling and rupture of the T-system is due to the rapid water movements that were observed during the onset of glycerol removal. Ultrastructural analysis of glycerol-treated atrium from the same hearts shows damage of mitochondria and of the L-system and intracellular edema. The structural changes are correlated with a loss of atrial contraction. As in ventricular myocardium, resumption of contraction is associated with an almost complete recovery from ultrastructural damage.

This is a preview of subscription content, log in to check access.

References

  1. Blanchi, C.P., Bolton, T.C.: Effect of Hypertonic solutions and glycerol treatment on calcium and magnesium movements of frog skeletal muscle. J. Pharmacol. Ther. 188, 536–552 (1974)

  2. Burnstock, G.: Structure of smooth muscle and its innervation. In: Smooth muscle, ed. by Bülbring, E., Brading, A.F., Jones, A.W., and Tomita, T., pp. 1–69. London: Edward Arnold Ltd., 1970

  3. Daldrup, J., Forssmann, W.G.: Elektronenmikroskopische Untersuchungen eines reversiblen Herzstillstandes nach Glycerinbehandlung in vitro. Verh. anat. Ges. (Jena) 130, 141–149 (1972)

  4. Dulhunty, A.F., Gage, P.W.: Differential effects of glycerol treatment on membrane capacity and excitation-contraction coupling in toad sartorius fibres. J. Physiol. (Lond.) 234, 373–408 (1973)

  5. Duckies, S. P., Jensen, R.A.: Effects of glycerol treatment on contractility and transmembrane potentials in cardiac tissue. J. molec. cell. Cardiol. 4, 49–58 (1972)

  6. Ebashi, S.: Excitation-contraction coupling. Ann. Rev. Physiol. 38, 293–313 (1976)

  7. Eisenberg, B., Eisenberg, R.S.: Selective disruption of the sarcotubular system in frog sartorius muscle. J. Cell Biol. 39, 451–567 (1968)

  8. Fawcett, P.W., Selby, C.C.: Observations on the fine structure of the turtle atrium. J. biophys. biochem. Cytol. 4, 63–71 (1958)

  9. Forssmann, W.G., Girardier, L.: A study of the T-system in rat heart. J. Cell Biol. 44, 1–19 (1970)

  10. Fuchs, F.: Striated muscle. Ann. Rev. Physiol. 36, 461–502 (1974)

  11. Fujino, M.: Physiological properties of single fiber from frog skeletal muscle under the influence of hypertonic urea-Ringer with particular regard to the structural flexibility of transverse tubular system. Proc. intern. Union physiol. Sci. 9, 557 (1971)

  12. Fujino, M., Yamaguchi, R., Fujino, S.: Glycerol effect in various kinds of muscle cell. Jap. J. Physiol. 22, 477–489 (1972)

  13. Fujino, M., Yamaguchi, T., Suzuki, K.: Glycerol effect and the mechanism linking excitation of the plasma membrane with contraction. Nature (Lond.) 192, 1159–1161 (1961)

  14. Hibbs, R.G., Ferrans, V.J.: An ultrastructural and histochemical study of rat atrial myocardium. Am. J. Anat. 124, 251–280 (1969)

  15. Holland, W.C., Porter, M.T.: Pharmacological effects of drugs on excitation-contraction coupling in cardiac muscle. Fed. Proc. 28, 1663–1669 (1969)

  16. Howell, J.N.: A lesion of the transverse tubules of skeletal muscle. J. Physiol. (Lond.) 201, 515–533 (1969)

  17. Howell, J.N., Jenden, D.J.: T-tubules of skeletal muscle: morphological alterations which interrupt excitation-contraction coupling. Fed. Proc. 26, 553 (1967)

  18. Karnovsky, M.J.: Use of ferrocyanide-reduced osmium tetroxide in electron microscopy. J. Cell Biol. 51, ASCB abstr., 146 (1971)

  19. Katzung, B.G., Teitelbaum, P.: Ionic effects of glycerol removal in cardiac muscle. J. molec. cell. Cardiol. 6, 179–186 (1974)

  20. Krolenko, S.A.: Vacuolization of skeletal muscle fibres. II. Localization of ferritin and the ultrastructure of the fibres. Cytology (USSR) 10, 804–811 (1968)

  21. Krolenko, S.A.: Changes in the T-system of muscle fibres under the influence of influx and efflux of glycerol. Nature (Lond.) 21, 966–968 (1969)

  22. Krolenko, S.A., Adamjan, S.J., Shwinka, N.E.: Vacuolization of skeletal muscle fibres. I. Vacuolization after efflux of low molecular non-electrolytes. Cytology (USSR) 9, 1346–1353 (1967)

  23. Luft, J.H.: Improvements in epoxy resin embedding methods. J, biophys. biochem. Cytol. 9, 409–424 (1961)

  24. McNutt, N.S., Fawcett, P.W.: The ultrastructure of the cat myocardium. II. Atrial muscle. J. Cell Biol. 42, 46–67 (1969).

  25. Nakajima, S., Nakajima, Y., Peachey, L.D.: Speed of repolarization and morphology of glycerol treatment muscle fibres. J. Physiol. (Lond.) 200, 115–116 (1969)

  26. Niemeyer, G., Forssmann, W.G.: Comparison of glycerol treatment in frog skeletal muscle and mammalian heart. J. Cell Biol. 50, 288–299 (1971)

  27. Porter-Sanders, M.T., Holland, W.C., Wassermann, O.: Effect of glycerol on atrial contractions and ultrastructure. Arch. int. Pharmacodyn. Ther. 182, 112–120 (1969)

  28. Reynolds, E.S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)

  29. Schmidt, E., Wilkes, A.B., Holland, W.C.: Effects of various glycerol or urea concentrations and incubation times on atrial contractions and ultrastructure. J. molec. cell. Cardiol. 4, 113–120 (1972)

  30. Simpson, F.O., Rayns, D.G., Ledingham, J.K.: The ultrastructure of ventricular and atrial myocardium. In: Ultrastructure in biological systems (Challice, C.E. and Viragh, S., eds.), Vol. 6, pp. 2–36 New York and London: Academic Press 1973

  31. Strosberg, A.M., Katzung, B.G., Lee, J.C.: Glycorol removal treatment of guinea pig cardiac muscle. J. molec. cell. Cardiol. 4, 39–40 (1972)

  32. Weibel, E.R., Kistler, G.S., Scherle, W.F.: Practical stereological methods for morphometric cytology. J. Cell Biol. 30, 23–38 (1966)

  33. Weihe, E.: Untersuchungen des Rattenherzmuskels bei Glycerinbehandlung. Inaugural-Dissertation mediz. Fak. Heidelberg (1973)

  34. Weihe, E., Heuck-Daldrup, J., Greiner, J., Niemeyer, G., Hartschuh, W., Forssmann, W.G.: Experimental tubulus rupture in rat myocardium by glycerol removal treatment. In: Adaptability of cardiac muscle. International Symposium, Abstracts, Prague 1974

Download references

Author information

Correspondence to Dr. E. Weihe.

Additional information

The studies were supported by the German Research Foundation within the SFB 90 “Cardiovasculäres System”

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Weihe, E., Hartschuh, W., Kalmbach, P. et al. The effects of glycerol treatment on the isolated rat heart. Cell Tissue Res. 185, 43–62 (1977). https://doi.org/10.1007/BF00226667

Download citation

Key words

  • Isolated perfused heart (rat)
  • Glycerol treatment
  • Ultrastructure
  • Electrolytes
  • Inulin space