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Theoretical and Applied Genetics

, Volume 83, Issue 2, pp 250–256 | Cite as

Construction of an RFLP map of barley

  • A. Graner
  • A. Jahoor
  • J. Schondelmaier
  • H. Siedler
  • K. Pillen
  • G. Fischbeck
  • G. Wenzel
  • R. G. Herrmann
Originals

Summary

In order to construct an RFLP map of barley, two populations were analyzed using 251 genomic and cDNA markers: one population comprised 71 F1 antherderived double haploid (DH) individuals of an intraspecific cross (IGRI x FRANKA), and the other 135 individuals of an interspecific F2/F3 progeny (VADA x H. spontaneum). The distribution of nonrepetitive clones over the seven barley chromosomes revealed a maximum for chromosome 2H and a minimum for 6H. The polymorphism of the interspecific progeny (76%) clearly exceeded that of the intraspecific progeny (26%) although, based on their pedigrees, IGRI and FRANKA are only distantly related. The contribution of individual chromosomes of the DH parents to the overall polymorphism varied between 8% and 50%. A significant portion (44% versus 10% of the interspecific progeny) of the markers mapped on the DH offspring showed distorted segregation, caused mainly by the prevalence of variants originating from the parent that better responded to in vitro culture (IGRI). In contrast to the interspecific map, probes displaying skewed segregation were clustered on the DH map on discrete segments. The colinear arrangement of both maps covers a distance of 1,453 cM and identifies regions of varying map distances.

Key words

Restriction fragment length polymorphism Linkage map Hordeum vulgare Double haploids 

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References

  1. Barone A, Ritter E, Schachtschabel U, Debener T, Salamini F, Gebhardt C (1990) Localization by restriction fragment length polymorphism mapping in potato of a major dominant gene conferring resistance to the potato cyst nematode Globoder a rostochiensis. Mol Gen Genet 224:177–182Google Scholar
  2. Bonierbale MW, Plaisted RL, Tanksley SD (1988) RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120:1095–1103Google Scholar
  3. Clapham D (1973) Haploid Hordeum plants from anthers in vitro. Z Pflanzenzuecht 69:142–155Google Scholar
  4. Debener T, Salamini F, Gebhardt C (1990) Phytogeny of wild and cultivated Solanum species based on nuclear restriction fragment length polymorphisms (RFLPs). Theor Appl Genet 79:360–368Google Scholar
  5. Deimling S, Zitzlsperger J, Wenzel G (1988) Somatic fusion for breeding of tetraploid potatoes. Plant Breed 101:181–189Google Scholar
  6. Foroughi-Wehr B, Friedt W (1984) Rapid production of recombinant barley yellow mosaic virus resistant Hordeum vulgare lines by anther culture. Theor Appl Genet 67:377–382Google Scholar
  7. Ganal MW, Young ND, Tanksley SD (1989) Pulsed field gel electrophoresis and physical mapping of large DNA fragments in the Tm-2a region of chromosome 9 in tomato. Mol Gen Genet 215:395–400Google Scholar
  8. Gebhardt C, Ritter E, Debener T, Schachtschabel U, Walkemeier B, Uhrig H, Salamini F (1989) RFLP analysis and linkage mapping in Solanum tuberosum. Theor Appl Genet 78:65–75Google Scholar
  9. Graner A, Siedler H, Jahoor A, Herrmann RG, Wenzel G (1990) Assessment of the degree and type of restriction fragment length polymorphism in barley (Hordeum vulgare). Theor Appl Genet 80:826–832Google Scholar
  10. Helentjaris T, Slocum M, Schaefer A, Nienhuis J (1986) Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphisms. Theor Appl Genet 72:761–769Google Scholar
  11. Islam AKMR, Shepherd KW, Sparrow DHB (1981) Isolation and characterization of euplasmic wheat-barley chromosome addition lines. Heredity 46:161–174Google Scholar
  12. Jahoor A, Fischbeck G (1987 a) Genetical studies of resistance of powdery mildew in barley lines derived from Hordeum spontaneum collected from Israel. Plant Breed 99:265–273Google Scholar
  13. Jahoor A, Fischbeck G (1987b) Sources of resistance to powdery mildew in barley lines derived from Hordeum spontaneum collected in Israel. Plant Breed 99:274–281Google Scholar
  14. Jahoor A, Backes G, Graner A, Herrman RG, Fischbeck G (1991) Development of RFLP markers for the barley genome. Plant Breed 107:73–76Google Scholar
  15. Jung C, Kleine M, Fischer F, Herrmann RG (1990) Analysis of DNA from a Beta procumbens chromosome fragment in sugar beet carrying a gene for nematode resistance. Theor Appl Genet 79:663–672Google Scholar
  16. Kahler AL, Allard RW (1970) Genetics of isozyme variants in barley. I. Esterases. Crop Sci 10:444–448Google Scholar
  17. Kasha KJ, Kao KN (1970) High frequency haploid production in barley (Hordeum vulgare L.). Nature 225:874–876Google Scholar
  18. Klein-Lankhorst R, Rietveld P, Machiels B, Verkerk R, Weide R, Gebhardt C, Koorneef M (1991) RFLP markers linked to the root knot nematode gene Mi in tomato. Theor Appl Genet 81:661–667Google Scholar
  19. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175Google Scholar
  20. Kuhlmann U, Foroughi-Wehr B (1989) Production of doubled haploid lines in frequencies sufficient for barley breeding programs. Plant Cell Rep 8:78–81Google Scholar
  21. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181PubMedGoogle Scholar
  22. Lüdecke HJ, Senger G, Claussen U, Horsthemke B (1990) Construction and characterization of band-specific DNA libraries. Human Genet 84:512–516Google Scholar
  23. MacDonald RJ, Swift GH, Przybyla AE, Chirgwin JM (1987) Isolation of RNA using guanidinium salts. In: Berger SL, Kimmel AR (eds) Guide to molecular cloning techniques. Academic Press, Orlando/FA, pp 219–226Google Scholar
  24. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press Cold Spring Harbor/NYGoogle Scholar
  25. McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829Google Scholar
  26. Melchinger AE, Messmer MM, Lee M, Woodman WL, Lamkey KR (1991) Diversity and relationships among U.S. maize inbreds revealed by restriction fragment length polymorphisms. Crop Sci 31:669–678Google Scholar
  27. Miller JC, Tanksley SD (1990) RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon. Theor Appl Genet 80:437–448Google Scholar
  28. Murray MG, Chyi YS, Cramer JH, DeMars S, Kirschmann J, Ma Y, Pitas J, Romero-Severson J, Shoemaker J, West DP, Zaitlin D (1991) Application of restriction fragment length polymorphism to maize breeding. In: Herrmann RG, Larkins B (eds) Plant molecular biology 1990/91. Plenum Publishing, Cambridge/NY (in press)Google Scholar
  29. Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726CrossRefPubMedGoogle Scholar
  30. Powell W, Borrino EM, Allison MJ, Griffiths DW, Asher MJC, Dunnwell JM (1986) Genetical analysis of microspore-derived plants of barley (Hordeum vulgare). Theor Appl Genet 72:619–626Google Scholar
  31. Rommens JM, Iannuzzi MC, Kerem BS, Drumm ML, Melmer G, Dean M, Rozmahel R, Cole JL, Kennedy D, Hidaka N, Zsiga M, Buchwald M, Riordan JR, Tsui LC, Collins FS (1989) Identification of the cystic fibrosis gene: Chromosome walking and jumping. Science 245:1059–1065Google Scholar
  32. Sarfatti M, Katan J, Fluhr R, Zamir D (1989) An RFLP marker in tomato linked to the Fusarium oxysporum resistance gene I2. Theor Appl Genet 78:755–759Google Scholar
  33. Shin JS, Chao S, Corpuz L, Blake T (1990) A partial map of the barley genome incorporating restriction fragment length polymorphism, polymerase chain reaction, isozyme and morphological marker loci. Genome 33:803–810Google Scholar
  34. Siedler H, Graner A (1991) Construction of physical maps of the Hor1 locus of two barley cultivars by pulsed field gel electrophoresis. Mol Gen Genet 226:177–181Google Scholar
  35. Slocum MK, Figdore SS, Kennard WC, Suzuki JY, Osborn TC (1990) Linkage arrangement of restriction fragment length polymorphism loci in Brassica oleracea. Theor Appl Genet 80:57–64Google Scholar
  36. Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding — new tools for an old science. Bio/Technol 7:257–264Google Scholar
  37. Thompson DM, Chalmers K, Waugh R, Fordter BP, Thomas WTB, Caligari PDS, Powell W (1991) The inheritance of genetic markers in microspore-derived plants of barley Hordeum vulgare L. Theor Appl Genet 81:487–492Google Scholar
  38. von Wettstein-Knowles P (1991) Cloned and mapped genes: current status. In: Shewry P (ed) Barley: genetics, molecular biology and biotechnology. CAB International, in pressGoogle Scholar
  39. Yu ZH, Mackill DJ, Bonmann JM, Tanksley SD (1991) Tagging genes for blast resistance in rice via linkage to RFLP markers. Theor Appl Genet 81:471–476Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • A. Graner
    • 1
  • A. Jahoor
    • 2
  • J. Schondelmaier
    • 2
  • H. Siedler
    • 1
  • K. Pillen
    • 3
  • G. Fischbeck
    • 2
  • G. Wenzel
    • 1
  • R. G. Herrmann
    • 3
  1. 1.Federal Biological Research Center for Agriculture and Forestry, Institute for Resistance GeneticsGrünbachFRG
  2. 2.Technical University Munich, Institute for Plant BreedingFreising-WeihenstephanFRG
  3. 3.Ludwig-Maximilians-University, Institute of BotanyMunich 19FRG

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