Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Encoding genes for endosperm proteins in Hordeum chilense

  • 48 Accesses

  • 16 Citations


The endosperm proteins encoded by the genome Hch in Hordeum chilense, Tritordeum (amphiploid Hordeum chilense x Triticum turgidum), common wheat-H. chilense addition lines, and the segregating plants resulting from the cross Tritordeum x T. turgidum, were fractionated by three electrophoretical techniques: SDS-PAGE, A-PAGE, and bidimensional PAGE. Prolamin subunits with a high molecular weight (HMW) were well visualized by SDS-PAGE, the A-PAGE technique permitted good resolution for many hordeins and gliadins, and two-dimensional electrophoresis allowed new sets of bands coded by gene complexes from H. chilense chromosomes to be distinguished. The loci Hor-Hch1 (up to 11 subunits belonging to the ω-, β — and α-hordeins), Glu-Hch1 (one HMW prolamin subunit), Hor-Hch2 (one α-hordein), and Hor-Hch3 (up to four α-hordeins) were located on the H. chilense chromosomes 1Hch, 5Hch, and 7Hch.

This is a preview of subscription content, log in to check access.


  1. Ainsworth CC, Gale MD, Baird S (1984) The genetic control of grain esterases in hexaploid wheat. 1. Allelic variation. Theor Appl Genet 8:219–226

  2. Ainsworth CC, Gale MD, Miller TE (1986) Genetic control of grain esterases in hexaploid wheat. 2. Homoeologous loci in related species. Theor Appl Genet 72:219–225

  3. Benito C, Frade JM, Orellana J, Carrillo JM (1990) Linkage and cytogenetic maps of genes controlling endosperm storage proteins and isozymes in rye (Secale cereale L.). Theor Appl Genet 79:347–352

  4. Chapman V, Miller TE (1978) The amphiploid of Hordeum chilense (x) Triticum aestivum. Cereal Res Commun 6:351–352

  5. Chojecki AJS, Gale MD (1982) Genetic control of glucose phosphate isomerase in wheat and related species. Heredity 49:337–347

  6. Fernández JA (1989) Genetics of Hordeum chilense — current status. Plant Breed 102:10–16

  7. Fernández JA, Jouve N (1987a) Chromosomal location of structural genes controlling isozymes in Hordeum chilense. 1. 6-phosphogluconate dehydrogenase and malate dehydrogenase. Theor Appl Genet 73:433–439

  8. Fernández JA, Jouve N (1987b) Chromosomal location of structural genes controlling isozymes in Hordeum chilense. 3. Esterases, glutamate oxaloacetate transaminase, and phosphoglucomutase. Theor Appl Genet 73:690–698

  9. Fernández JA, Jouve N (1988) The addition of Hordeum chilense chromosomes to Triticum turgidum conv. durum. Biochemical, karyological, and morphological characterization. Euphytica 37:247–259

  10. Fernández JA, Jouve N (1990) Chromosomal location of genes encoding a leaf phosphatase and a cathodal peroxidase in the wild barley Hordeum chilense. J Hered (in press)

  11. Galili G, Feldman M (1984) Mapping of glutenin and gliadin genes located on chromosome 1B of common wheat. Mol Gen Genet 193:293–298

  12. Lafiandra D, Kasarda D (1985) One- and two-dimensional (two-pH) polyacrylamide gel electrophoresis in a single gel separation of wheat proteins. Cereal Chem 62:314–319

  13. Martin A, Chapman V (1977) A hybrid between Hordeum chilense and Triticum aestivum. Cereal Res Commun 9:317–323

  14. Martin A, Cubero JI (1981) The use of Hordeum chilense in cereal breeding. Cereal Res Commun 9:317–323

  15. Martiń A, Sánchez-Monge Laguna E (1982) Cytology and morphology of the amphiploid Hordeum chilense x Triticum turgidum conv. durum. Euphytica 31:261–267

  16. Martín A, Sánchez-Monge Laguna E (1985) Effects of the 5B system on control of pairing in Hordeum chilense x Triticum aestivum hybrids. Z Pflanzenzuecht 85:122–127

  17. Miller TE, Reader SM, Chapman V (1982) The addition of Hordeum chilense chromosomes to wheat. Induced variability in plant breeding. Eucarpia Int Symp, 1981. Pudoc, Wageningen, pp 79–81

  18. Miller TE, Reader SM, Ainsworth CC (1985) A chromosome of Hordeum chilense homoeologous to group 7 of wheat. Can J Genet Cytol 27:101–104

  19. Payne PI, Corfield KG, Holt LM, Blackman JA (1981) Correlations between the inheritance of certain high-molecular-weight subunits of glutenin and bread-making quality in progeny of six crosses of bread wheat. J Sci Food Agric 32:51–60

  20. Payne PI, Jackson LM, Holt LM, Law CN (1984) Genetic linkage between endosperm protein genes of each of the short arms of chromosomes 1A and 1B in wheat. Theor Appl Genet 67:235–243

  21. Payne PI, Holt LM, Reader SM, Miller TE (1987) Chromosomal location of genes coding for endosperm proteins of Hordeum chilense determined by two-dimensional electrophoresis of -wheat/H. chilense chromosome addition lines. Biochem Genet 25:53–65

  22. Shewry PR, Bradberry D, Franklin J, White RP (1984) The chromosomal locations and linkage relationships of the structural genes for prolamin storage proteins (secalins) of rye. Theor Appl Genet 69:63–69

  23. Shewry PR, Tatham AS, Field JM, Forde BC, Clarck J, Gallois P, Marris C, Halford M, Forde J, Kreis M (1988) The structures of barley and wheat prolamins and their genes. Biochem Physiol Pflanz 183:117–127

  24. Sybenga J, Parmar S, Eden J van, Shewry P (1990) Mapping seed storage protein loci Sec-1 and Sec-3 in relation to five chromosomal rearrangements in rye (Secale cereale L.). Theor Appl Genet 79:201–208

Download references

Author information

Additional information

Commicated by J. Mac Key

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Tercero, J.A., Bernardo, A. & Jouve, N. Encoding genes for endosperm proteins in Hordeum chilense . Theoret. Appl. Genetics 81, 127–132 (1991).

Download citation

Key words

  • Hordeum
  • A-PAGE
  • Two-dimensional electrophoresis
  • Hordeins