Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Long-range organization of a satellite DNA family flanking the beet cyst nematode resistance locus (Hs1) on chromosome-1 of B. patellaris and B. procumbens


New members of a satellite DNA family (Sat 121), specific for wild beets of the section Procumbentes of the genus Beta, were isolated. Sequence analysis showed that the members of Sat-121 fall into two distinct classes. The organization of Sat-121 in the vicinity of a beet cyst nematode (Heterodera schachtii Schm.) resistance locus (Hs1) in B. patellaris and B. procumbens was investigated by pulsed-field gel electrophoresis (PFGE) using DNA from a series of resistant monosomic fragment additions, each containing an extra chromosome fragment of B. patellaris chromosome-1 (pat-1) in B. vulgaris. In this way several clusters of Sat-121 flanking the Hs1 pat-1locus were identified. In nematode resistant diploid introgressions (2n=18), which contain small segments of B. procumbens chromosome-1 (pro-1) in B. vulgaris, only two major Sat-121 clusters were detected near the Hs1 pro-1locus.

This is a preview of subscription content, log in to check access.


  1. Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–218

  2. De Jong JH, Speckmann GJ, De Bock ThSM, Lange W, Van Voorst A (1986) Alien chromosome fragments conditioning resistance to beet cyst nematode in diploid descendants from monosomic additions of Beta procumbens to B. vulgaris. Can J Genet Cytol 28:439–443

  3. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA mini-preparation: version II. Plant Mol Biol Rep 4:19–21

  4. Flavell R (1986) Repetitive DNA and chromosome evolution in plants. Phil Trans R Soc Lond B 312:227–242

  5. Flavell R, Bennett MD, Smith JB, Smith OB (1974) Genome size and the proportion of repeated nucleotide sequence DNA in plants. Biochem Genet 12:257–269

  6. Hake S, Walbot V (1980) The genome of Zea mays, its organization and homology to related grasses. Chromosoma 79:251–270

  7. Heijbroek W, Roelands AJ, De Jong JH, Van Hulst CG, Schoone AHL, Munning RG (1988) Sugar beets homozygous for resistance to the beet cyst nematode (Heterodera schachtii Schm.), developed from monosomic additions of B. procumbens to B. vulgaris. Euphytica 38: 121–131

  8. Hörz W, Zachau HG (1977) Characterization of distinct segments in mouse satellite DNA by restriction nucleases. Eur J Biochem 73:383–392

  9. Jung C, Herrmann RG (1991) A DNA probe for rapid screening of sugar beet (Beta vulgaris L.) carrying extra chromosomes from wild beets of the Procumbentes section. Plant Breed 107:275–279

  10. Jung C, Kleine M, Fischer F, Herrmann RG (1990) Analysis of DNA from a B. procumbens chromosome fragment in sugar beet carrying a gene for nematode resistance. Theor Appl Genet 79:663–672

  11. Jung C, Koch R, Fisher F, Brandes A, Wricke G, Herrmann RG (1992) DNA markers closely linked to nematode resistance genes in sugar beet (Beta vulgaris L.) mapped using chromosome additions and translocations originating from wild beets of the Procumbentes section. Mol Gen Genet 232:271–278

  12. Krens FA, Jamar D, Rouwendaal GJA, Hall RD (1990) Transfer of cytoplasm from new CMS sources to sugar beet by asymmetric fusion. 1. Shoot regeneration from mesophyll protoplasts and characterization of regenerated plants. Theor Appl Genet 79:390–396

  13. Lange W, Jung C, Heijbroek W (1990) Transfer of beet cyst nema tode resistance from Beta species of the section Patellares to cultivated beet. Proc 53rd Winter Congr. International Institute for Sugar Beet Research, pp 89–102

  14. Lange W, Müller J, De Bock ThSM (1993) Virulence in the beet cyst nematode (Heterodera schachtii) versus some alien genes for resistance in beet. Fundam Appl Nematol 16:447–454

  15. Lapitan NLV (1991) Organization and evolution of higher plant nuclear genomes. Genome 35:171–180

  16. Leutwiler LS, Hough-Evans BR, Meyerowitz EM (1984) The DNA of Arabidopsis thaliana. Mol Gen Genet 194:15–23

  17. Maluszynska J, Heslop-Harrison JS (1991) Localization of tandemly repeated DNA sequences in Saccharomyces cerevisiae. Curr Genet 12:1–7

  18. Röder MS, Lapitan NVL, Sorrells ME, Tanksley SD (1993) Genetic and physical mapping of barley telomeres. Mol Gen Genet 238:294–303

  19. Salentijn EMJ, Sandal NN, Lange W, De Bock ThSM, Krens FA, Marcker KA, Stiekema WJ (1992) Isolation of DNA markers linked to a beet cyst nematode resistance locus in Beta patellaris and Beta procumbens. Mol Gen Genet 235:432–440

  20. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

  21. Santoni S, Bervillé A (1992). Two different satellite DNAs in B. vulgaris L.: evolution, quantification and distribution in the genus. Theor Appl Genet 84:1009–1016

  22. Savitsky H, Price C (1965) Resistance to sugarbeet nematode (Heterodera schachtii) in F1 tetraploid hybrids between B. vulgaris and B. patellaris. Am Soc Sugar Beet Technol 13:370–373

  23. Schmidt T, Jung C, Metzlaff M (1991) Distribution and evolution of two satellite DNAs in the genus Beta. Theor Appl Genet 82:793–799

  24. Schmidt T, Metzlaff M (1991) Cloning and characterization of a Beta vulgaris satellite DNA family. Gene 101:247–250

  25. Schmidt T, Junghans H, Metzlaff M (1990) Construction of B. procumbens-specific DNA probes and their application for the screening of B. vulgaris × B. procumbens (2n=19) addition lines. Theor Appl Genet 79:177–181

  26. Schwartz DC, Cantor CR (1984) Separation of yeast chromosomesized DNAs by pulsed-field-gradient gel electrophoresis. Cell 37:67–75

  27. Speckmann GJ, De Bock ThSM, De Jong JH (1985) Monosomic additions with resistance to beet cyst nematode obtained from hybrids of B. vulgaris and wild Beta species of the section Patellares. Z Pflantzenzucht 95:74–83

  28. Van Daelen RAJ, Jonkers JJ, Zabel P (1989) Preparation of megabase-sized tomato DNA and separation of large restriction fragments by field-inversion gel electrophoresis (FIGE). Plant Mol Biol 12:341–352

  29. Wicking C, Williamson B (1991) From linked marker to gene. Trends Genet 7:288–293

  30. Yu MH (1978) Meiotic behaviour of a disomic nematode resistant sugar beet. Crop Sci 18:615–618

Download references

Author information

Correspondence to W. J. Stiekema.

Additional information

Communicated by G. Wenzel

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Salentijn, E.M.J., Sandal, N.N., Klein-Lankhorst, R. et al. Long-range organization of a satellite DNA family flanking the beet cyst nematode resistance locus (Hs1) on chromosome-1 of B. patellaris and B. procumbens . Theoret. Appl. Genetics 89, 459–466 (1994).

Download citation

Key words

  • Molecular markers Beet cyst nematode resistance
  • Hs1 Heterodera schachtii Schm
  • Satellite DNA Monosomic fragment additions Beta
  • Pulsed-field gel electrophoresis