Intranuclear and cytoplasmic annulate lamellae were studied in grasshopper spermatocytes (Melanoplus) with the electron microscope. Although cytoplasmic annulate lamellae were observed in all three species examined, intranuclear annulate lamellae were found in only one species. The intranuclear annulate lamellae encompass certain nuclear material adjacent to the nuclear envelope forming a vesicle that is extruded into the spermatocyte cytoplasm. In this same species, cytoplasmic annulate lamellae are seen contiguous with granular masses of varying size. These structures were noted as being morphologically indistinguishable from the “yolk nuclei” of dragonfly oocytes (Kessel and Beams, 1969; Kessel, 1973).
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Benzo, C. A.: The annulate lamellae of chick embryo liver cells in organ culture. Anat. Rec. 174, 399–406 (1972)
Benzo, C. A.: Annulate lamellae in hepatic and pancreatic beta cells of the chick embryo. Amer. J. Anat. 140, 139–142 (1974)
Comings, D. E., Okada, T. A.: The chromatoid body in mouse spermatogenesis: Evidence that it may be formed by the extrusion of nucleolar components. J. Ultrastruct. Res. 39, 15–23 (1972)
Conway, C. M., Metz, C. B.: In vitro maturation of Arbacia punetulata oocytes and initiation of heavy body formation. An ultrastructural study. Cell Tiss. Res. 150, 271–279 (1974)
Dhainaut, A.: Mode de formation des lamelles annelées resultant de l'évolution, en condition anhormonale, des ovocytes de Nereis diversicolor (Annélide Polychète). Z. Zellforsch. 137, 481–492 (1973)
Franke, W. W., Ulrich, S., Fritsch, H.: Intranuclear and cytoplasmic annulate lamellae in plant cells. J. Cell Biol. 53, 823–827 (1972)
Gulyas, B. J.: The rabbit zygote II. The fate of annulate lamellae during first cleavage. Z. Zellforsch. 133, 187–200 (1972)
Hoage, T. R., Kessel, R. G.: An electron microscope study of the process of differentiation during spermatogenesis in the drone honey bee (Apis mellifera L.) with special reference to centriole replication and elimination. J. Ultrastruct. Res. 24, 6–23 (1968)
Jollie, W. P.: Nuclear and cytoplasmic annulate lamellae in trophoblast giant cells of rat placenta. Anat. Rec. 165, 1–14 (1969)
Kessel, R. G.: Annulate lamellae. J. Ultrastruct. Res., Suppl. 10, 1–82 (1968)
Kessel, R. G.: Structure and function of the nuclear envelope and related cytomembranes. In: Progress in Surface and Membrane Science 6, 243–329 (1973)
Kessel, R. G., Beams, H. W.: Annulate lamellae and “yolk nuclei” in oocytes of the dragonfly, Libellula pulchella. J. Cell Biol. 42, 185–201 (1969)
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961)
Maul, G.: On the origin of annulate lamellae in human melanoma cells in vitro. J. Cell Biol. 39, 88a (1968)
Maul, G. G., Cross, P. C.: Thymidine incorporation by annulate lamellae of rabbit zygotes. J. Ultrastruct. Res. 47, 115–124 (1974)
Ollerich, D. A., Carlson, E. G.: Ultrastructure of intranuclear annulate lamellae in giant cells of rat placenta. J. Ultrastruct. Res. 30, 411–423 (1970)
Picheral, B., Folliot, R.: Sur un nouvel element intracisternal de la structure du pore de l'enveloppe nucléaire et des lamelles annelées. J. Microscopie 12, 459–462 (1971)
Pope, R. S., Ollerich, D. A.: Intranuclear annulate lamellae in giant cells of the mouse chorioallantoic placenta. Anat. Rec. 176, 101–110 (1973)
Rebhun, L. I.: Some electron microscope observations on membranous basophilic elements of invertebrate eggs. J. Ultrastruct. Res. 5, 208–225 (1961)
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–213 (1963)
Scheer, U., Franke, W. W.: Negative staining and adenosine triphosphatase activity of annulate lamellae of newt oocytes. J. Cell Biol. 42, 519–533 (1969)
Scheide, O. A., Nicholls, T., Graham, G.: Annulate lamellae and chromatoid bodies in the testes of a cyprinid fish (Pimephales notatus). Z. Zellforsch. 129, 1–10 (1972)
Watson, M. L.: Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol. 4, 475 (1958)
Wilson, E. B.: The cell in development and heredity, third edit. New York: The Macmillan Co. 1925
Wischnitzer, S.: The annulate lamellae. Int. Rev. Cytol. 27, 65–100 (1970)
This work was completed in the Electron Microscope Laboratory, Department of Biology, Sam Houston State University, Huntsville, Texas 77340. Supported in part by grants from the Philosophical Society of America (JF#1012) and the National Science Foundation (GB23915). The authors are indebted to Dr. J. N. Dent for his critical evaluation of this manuscript.
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Pool, T.B., Hoage, T.R. Intranuclear and cytoplasmic annulate lamellae in grasshopper spermatocytes (Genus Melanoplus). Cell Tissue Res. 156, 475–482 (1975). https://doi.org/10.1007/BF00225107
- Annulate lamellae
- Electron microscopy