A mitochondrial plasmid of 1,939 bp (P2) from a cytoplasmic male-sterile line of sunflower has been cloned and sequenced. It presents 437 bp of near-perfect homology to the 1.4-kb mitochondrial plasmid P1 from sunflower. Sequences homologous to P2 were found in nuclear DNA. P2 was transcribed into a major 980-nucleotide (nt) RNA molecule and two minor transcripts of 570 and 520 nt. They were all transcribed from the same strand and within the region nonhomologous to P1. A single 5′ boundary and three 3′ termini were determined for P2 transcripts. The 5′ end is similar to a consensus sequence for plant mitochondrial genes. No evidence of translation products can be provided.
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de la Canal, L., Crouzillat, D., Flamand, M.C. et al. Nucleotide sequence and transcriptional analysis of a mitochondrial plasmid from a cytoplasmic male-sterile line of sunflower. Theoret. Appl. Genetics 81, 812–818 (1991). https://doi.org/10.1007/BF00224995
- Mitochondrial plasmids
- Mitochondrial DNA