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Electron microscopical microprobe analysis of freeze dried and unstained mineralized epiphyseal cartilage

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Summary

Electron micrographs have been taken of unfixed, freeze dried, unstained epiphyseal cartilage. In the mineralized long septa round to elliptic clusters (up to 0.6 μm in diameter) consisting mainly of dots and needles could be observed. The clusters were surrounded by microareas with a low contrast consisting mainly of ribbon plate-like crystallites. With the aid of scanning mode (STEM) of a transmission electron microscope, equipped with a Si(Li)detector system, both regions were analyzed for calcium and phosphorus by electronprobe X-ray microanalysis. In ten series of 106 measurements in each region, it could be determined by registration of the CaKα and PKαX-ray counts that the mineral content in the clusters was in the range of 30–100 % higher than in the light regions. The question of the sequence of the epiphyseal plate mineralization is discussed and whether the dense clusters represent the mineralized matrix vesicles.

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References

  1. Anderson, H.C.: Electron microscopic studies of induced cartilage development and calcification. J. Cell Biol. 35, 81–101 (1967)

  2. Bonucci, E.: Fine structure of early cartilage calcification. J. Ultrastruct. Res. 20, 33–50 (1967)

  3. Bonucci, E.: Fine structure and histochemistry of “calcifying globules” in epiphyseal cartilage. Z. Zellforsch. 103, 192–217 (1970)

  4. Höhling, H.J., Hall, T.A., Boyde, A., Rosenstiel, A.P. von: Combined electronprobe and electron diffraction analysis of prestages and early stages of dentine formation in rat incisor. Calif. Tiss. Res. 2, Suppl. 5 (1968)

  5. Höhling, H.J., Schöpfer, H., Höhling, R.A., Hall, T.A., Gieseking, R.: The organic matrix of developing tibia and femur. Naturwissenschaften 57, 357 (1970)

  6. Höhling, H.J., Steffens, H., Stamm, G., Mays, U.: Transmission microscopy of freeze dried, unstained epiphyseal cartilage of the guinea pig. Cell. Tiss. Res. 167, 243–263 (1976)

  7. Katchburian, E.: Initiation of mineral deposition in dentine. XII. European Symposium on Calcified Tissues, July 4–8, 1976, York/England

  8. Kushida, H.: A styrene-methacrylate resin embedding method for ultrathin sectioning. J. Electron microscopy 10, 16–19 (1961)

  9. Reimer, L.: Änderung des elektronenmikroskopischen Bildkontrastes beim Übergang amorphkristallin und flüssig-kristallin. Naturwissenschaften 49, 297 (1962)

  10. Reimer, L.: Elektronenmikroskopische Untersuchungs- und Präparationsmethoden, 2. Aufl., S. 167–170. Berlin-Heidelberg-New York: Springer 1967

  11. Spurr, A.R.: A low viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43 (1969)

  12. Umrath, W.: Cooling bath for rapid freezing in electron microscopy. J. Micr. 101, 103–105 (1974)

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Author information

Correspondence to Dr. R. H. Barckhaus.

Additional information

Dedicated to Professor Dr. G. Pfefferkorn on the occasion of his 65th birthday

The authors thank the Deutsche Forschungsgemeinschaft for financial support

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Barckhaus, R.H., Höhling, H.J. Electron microscopical microprobe analysis of freeze dried and unstained mineralized epiphyseal cartilage. Cell Tissue Res. 186, 541–549 (1978). https://doi.org/10.1007/BF00224942

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Key words

  • Epiphyseal plate
  • Cluster mineralization
  • Matrix vesicles
  • Electron microscopic microprobe