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Glycolytic enzymes in non-photosynthetic plastids of pea (Pisum sativum L.) roots

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Abstract

The presence of the glycolytic enzymes from hexokinase to pyruvate kinase in plastids of seedling pea (Pisum sativum L.) roots was investigated. The recoveries, latencies and specific activities of each enzyme in different fractions was compared with those of organelle marker enzymes. Tryptic-digestion experiments were performed on each enzyme to determine whether activities were bound within membranes. The results indicate that hexokinase (EC 2.7.1.2) and phosphoglyceromutase (EC 5.4.2.1) are absent from pea root plastids. The possible function of the remaining enzymes is considered.

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Abbreviations

GADPH:

glyceraldehyde 3-phosphate dehydrogenase

PFK:

phosphofructokinase

PFP:

pyrophosphate: fructose 6-phosphate 1-phosphotransferase

References

  1. ap Rees, T. Bryce, J.H., Wilson, P.M., Green, J.H. (1983) Role and location of NAD malic enzyme in thermogenic tissues of Araceae. Arch. Biochem. Biophys. 227, 511–521

  2. ap Rees, T., Green, J.H., Wilson, P.M. (1985) Pyrophosphate: fructose 6-phosphate 1-phosphotransferase and glycolysis in non-photosynthetic tissues of higher plants. Biochem. J. 227, 299–304

  3. Borchert, S., Grosse, H., Heldt H.W. (1989) Specific transport of inorganic phosphate, glucose 6-phosphate, dihydroxyacetone phosphate and 3-phosphoglycerate into amyloplasts from pea roots. FEBS Lett. 253, 183–186

  4. Bowsher, C.G., Hucklesby, D.P., Emes, M.J. (1989) Nitrite reduction and carbohydrate metabolism in plastids purified from roots of Pisum sativum L. Planta 177, 359–366

  5. Bowsher, C.G., Rose, J., Boulton, E.L., Nayagam, S., Emes, M.J. (1992) Reductant for glutamate synthase is generated by the oxidative pentose phosphate pathway in pea root plastids. Plant J. 2, 893–898

  6. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of protein utilizing the principal of protein-dye binding. Anal. Biochem. 72, 248–254

  7. Dennis, D.T. (1989) Fatty acid biosynthesis in plastids. In: Bio-chemistry and genetics of non-green plastids, pp. 120–129, Boyer, C.D., Shannon, J.C., Hardison, R.C., eds. American Society of Plant Physiologists, Rockville, Md.

  8. Dennis, D.T., Blakeley, S., Carlisle, S. (1991) In: Compartmentation of plant metabolism in non-photosynthetic tissues, pp. 77-94, Emes, M.J., ed. Cambridge University Press, UK

  9. Denyer, K., Smith, A.M. (1988) The capacity of plastids from developing pea cotyledons to synthesise acetyl CoA. Planta 173, 172–182

  10. Dry, I.B., Nash, I.B., Wiskich, J. (1983) The mitochondrial location of hexokinase in pea leaves. Planta 158, 152–156

  11. El-Shora, H.M., ap Rees, T. (1991) Intracellular location of NADP-linked malic enzyme in C3 plants. Planta 185, 362–367

  12. Emes, M.J., England, S. (1986) Purification of plastids from higher plant roots. Planta 168, 161–166

  13. Emes, M.J., Fowler, M.W. (1979a) The intracellular location of the enzymes of nitrate assimilation in the apices of seedling pea roots. Planta 144, 249–253

  14. Emes, M.J., Fowler, M.W. (1979b) Intracellular interactions between the pathways of carbohydrate oxidation and nitrate assimilation in plant roots. Planta 145, 287–292

  15. Emes, M.J., Traska, A. (1987) Uptake of inorganic phosphate by plastids purified from the roots of Pisum sativum L. J. Exp. Bot. 38, 1781–1788

  16. Entwistle, G., ap Rees, T. (1988) Enzymic capacities of amyloplasts from wheat (Triticum aestivum) endosperm. Biochem. J. 255, 391–396

  17. Fletcher, L. (1978) Studies on the heterogeneity of the glycolytic enzymes enolase and phosphofructokinase. Ph.D. thesis, University of Sheffield, UK

  18. Fowler, M.W., Clifton, A. (1975) Hexokinase activity in cultured sycamore cells. New Phytol. 75, 533–538

  19. Frehner, M., Pozueta-Romera, J., Akazawa, T. (1990) Enzyme sets of glycolysis, gluconeogenesis and oxidative pentose phosphate pathway, are not complete in non-green highly purified amyloplasts of sycamore (Acer pseudoplantus L.) cell suspension cultures. Plant Physiol. 94, 538–544

  20. Ireland, R.J., De Luca, V., Dennis, D.T. (1980) Characterisation and kinetics of pyruvate kinase from developing castor oil seed endosperm. Plant Physiol. 65, 1188–1193

  21. Journet, E.P., Douce, R. (1985) Enzymic capacities of purified cauliflower bud plastids for lipid synthesis and carbohydrate metabolism. Plant Physiol. 79, 458–467

  22. Joyard, J., Billecocq, A., Bartlett, S.G., Block, M.A., Chua, N.-H., Douce, R. (1983) Localisation of polypeptides to the cytosolic side of the outer envelope membrane of spinach chloroplasts. J. Biol. Chem. 258, 10000–10006

  23. Kleppinger-Sparace, K.F., Stahl, R.J., Sparace, S.A. (1992) Energy requirements for fatty acid and glycerolipid biosynthesis from acetate by pea root plastids. Plant Physiol. 98, 723–727

  24. Losada, M., Paneque, A. (1971) Nitrite reductase. Methods Enzymol. 23, 487–491

  25. MacDonald, F., ap Rees T. (1983) Enzymic properties of amyloplasts from suspension cultures of soybean. Biochim. Biophys. Acta 755, 81–89

  26. Miernyk, J.A., Dennis, D.T. (1984) Enolase isozymes from Ricinus communis. Partial purification and characterisation of the isozymes. Arch. Biochem. Biophys. 233, 643–651

  27. Miernyk, J.A., Dennis, D.T. (1992) A developmental analysis of the enolase isozymes from Ricinus communis. Plant Physiol. 99, 748–750

  28. Plaxton, W.C. (1988) Purification of pyruvate kinase from germinating castor bean endosperm. Plant Physiol. 86, 1064–1069

  29. Plaxton, W.C. (1990) Glycolysis. In: Methods in plant biochemistry, vol. 3: Enzymes of primary metabolism, pp. 145–147, Lea, P.J., ed. Plenum, London New York San Francisco

  30. Randall, D.D., Miernyk, J.A., Fang, T.K., Budde, R.J.A., Schulter, K.A. (1989) Regulation of the pyruvate dehydrogenase complexes in plants. Ann. N.Y. Acad. Sci. 573, 192–205

  31. Rutter, W.J., Richards, O.C., Woodfin, B.M. (1961) Comparative studies of liver and muscle aldolase. Effect of carboxypeptidase on catalytic activity. J. Biol. Chem. 236, 3193

  32. Schnarrenberger, C. (1990) Characterisation and compartmentation, in green leaves, of hexokinases with different specificities for glucose, fructose and mannose and for nucleoside triphosphates. Planta 181, 249–255

  33. Scopes, K.R. (1975) 3-phosphoglycerate kinase of skeletal muscle. Methods Enzymol. 42, 127–134

  34. Scott, K.J., Craigie, J.S., Smillie, R.M. (1964) Pathways of respiration in plant tumours. Plant Physiol. 39, 323–327

  35. Simcox, P.D., Reid, E.E., Canvin, D.T., Dennis, D.T. (1977) Enzymes of the glycolytic and pentose phosphate pathways in proplastids from the developing endosperm of Ricinus communis. Plant Physiol. 59, 1228–1232

  36. Sparace, S.A., Menassa, R., Kleppinger-Sparace, K.F. (1988) A preliminary analysis of fatty acid synthesis in pea roots. Plant Physiol. 87, 134–137

  37. Stitt, M., ap Rees, T. (1979) Capacities of pea chloroplasts to catalyse the oxidative pentose phosphate pathway and glycolysis. Phytochemistry 18, 1905–1911

  38. Tyson, R.H., ap Rees, T. (1988) Starch synthesis by isolated amyloplasts from wheat endosperm. Planta 175, 33–38

  39. Van Ginkel, G., Brown, J.S. (1978) Endogenous catalase and superoxide dismutase activities in photosynthetic membranes. FEBS Lett. 94, 284–28

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Bronwen A. Trimming gratefully acknowledges the award of a studentship from the Science and Engineering Research Council

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Trimming, B.A., Emes, M.J. Glycolytic enzymes in non-photosynthetic plastids of pea (Pisum sativum L.) roots. Planta 190, 439–445 (1993). https://doi.org/10.1007/BF00224781

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Key words

  • Glycolysis
  • Pisum (glycolysis)
  • Plastid (root)
  • Root (plastid, glycolysis)