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Morphology of cultured guinea-pig cerebellum

I. Pattern of development. Comparison of phase contrast cinematography and silver impregnations of various cell types

Summary

Guinea-pig cerebellum has been cultured in three in vitro systems: Maximow slides, Leighton tubes and Rose chambers. The best results were obtained from foetuses at the 34th day of gestation. Myelination appeared at the 21st day in vitro and progressed until after one month in culture. It was observed in 62% of the cultures on Maximow slides or in Leighton tubes. Myelination was quite variable in Rose chambers. The organization of the cultures was quite similar to that previously described in other species. Most types of neurons and glia were identified by phase contrast microscopy and microcinematographic analysis. Various histological stains and impregnations accompanied studies of living cells. Despite the lack of specificity of metal impregnations when applied to tissue cultures, successive study of similar or identical fields by phase contrast and such methods has facilitated cellular identification and demonstrated the limits of each of the techniques used. The difficulty of cell identification in the outgrowth zone is emphasized. Nevertheless, the following cell types have been identified:

    u
  • neurons: external granule cells, internal granule cells, Purkinje cells, roof nucleus type I and II neurons,

  • glial cells: immature cells, oligodendrocytes, astrocytes, histiocytic cells.

The guinea-pig cerebellum is characterized in vitro as it is in vivo by a particularly slow development when compared to other mammals.

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Correspondence to Dr. Jean-Jacques Hauw.

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Hauw, J., Boutry, J., Crosnier-Suttin, N. et al. Morphology of cultured guinea-pig cerebellum. Cell Tissue Res. 152, 141–164 (1974). https://doi.org/10.1007/BF00224691

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Key words

  • Cerebellum
  • Neurons
  • Neuroglia
  • Guinea pig
  • Tissue culture
  • Microcinematography