Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Ultrastructural studies on neurophysine-containing vesicles of the neurosecretory system of vertebrates

  • 15 Accesses

  • 9 Citations

Summary

A new method described in the present paper allows identification of neurophysine-containing (NP) vesicles of the magnocellular neurosecretory system of vertebrates. After oxidation of ultrathin sections, the content of NP vesicles can be dissolved specifically in an alkaline medium. This procedure marks NP vesicles selectively in all portions of the classical neurosecretory system.

This is a preview of subscription content, log in to check access.

References

  1. Bargmann, W., Gaudecker, Br. v.: Über die Ultrastruktur neurosekretorischer Elementargranula. Z. Zellforsch. 96, 495–504 (1969)

  2. Bargmann, W., Knoop, A.: Elektronenmikroskopische Untersuchungen an der Neurohypophyse. Z. Zellforsch. 46, 242–251 (1957)

  3. Barry, J., Dubois, M.P.: Étude en immunofluorescence des structures hypothalamiques à compétence gonadotrope. Ann. Endocrinol. 34, 735–742 (1973a)

  4. Barry, J., Dubois, M.P.: La voie neurosécrétrice preoptico-infundibulaire à LH-RH chez le cobaye au cours de la gestation. C.R. Soc. Biol. (Paris) 167, 1812–1816 (1973b)

  5. Barry, J., Dubois, M.P.: Immunfluorescence study of the preoptico-infundibular LH-RH neurosecretory pathway of the guinea pig during the estrous cycle. Neuroendocrinology 15, 200–208 (1974a)

  6. Barry, J., Dubois, M.P.: Étude au immunofluorescence de la différenciation prénatale des cellules hypothalamiques élaboratrices de LH-RH et de la maturation de la voie neurosécrétrice préoptico-infundibulaire chez le cobaye. Brain Res. 67, 103–113 (1974b)

  7. Björklund, A., Lindvall, O., Svensson, L.Å.: Mechanisms of fluorophore formation in the histochemical glyoxylic acid method for monoamines. Histochemie 32, 113–131 (1972)

  8. Calas, A., Kerdelhué, B., Assenmacher, I., Justisz, M.: Les axones à LH-RH de l'éminence médiane. Mise en évidence chez la Canard par une technique immunocytochimique. C.R. Acad. Sci. (Paris) 277, 2765–2768 (1973)

  9. Calas, A., Kerdelhué, B., Assenmacher, I., Justisz, M.: Les axones à LH-RH de l'éminence médiane. Etude ultrastructurale chez le Canard par une technique immunocytochimique. C.R. Acad. Sci. (Paris) 278, 2557–2560 (1974)

  10. Dierickx, K., Druyts, A., Vandenberghe, M.P., Goossens, N.: Identification of adenohypophysiotropic neurohormone producing neurosecretory cells in Rana temporaria. I. Ultrastructural evidence for the presence of neurosecretory cells in the tuber cinereum. Z. Zellforsch. 134, 459–504 (1972)

  11. Ekengren, B.: The nucleus preopticus and the nucleus lateralis tuberis in the roach, Leuciscus rutilus. Z. Zellforsch. 140, 369–388 (1973)

  12. Falck, B.: Observation on the possibilities of the cellular localization of monoamines by a fluorescence method. Acta physiol. scand. 56, Suppl. 197, 1–25 (1962)

  13. Ishii, S.: Classification and identification of neurosecretory granules in the median eminence. In: Brain-endocrine interaction. Median eminence: Structure and function (eds., K.M. Knigge, D.E. Scott, A. Weindl), p. 119–141. Basel: S. Karger 1972

  14. King, J.C., Persons, J.A., Erlandsen, S.L., Williams, T.H.: Luteinizing hormone-releasing hormone (LH-RH) pathway of the rat hypothalamus revealed by the unlabeled antibody peroxidaseantiperoxidase method. Cell Tiss. Res. 153, 211–217 (1974)

  15. Kuhlmann, D., Greven, H.: Licht- und fluoreszenzmikroskopische Darstellung spezifischer Zellen und Strukturen des Binde- und Nervengewebes von Gastropoden im Semidünnschnitt. Histochemie 34, 177–198 (1973)

  16. Leclerc, R., Pelletier, G.: Electron microscope immunhistochemical localization of vasopressin in the hypothalamus and neurohypophysis of the normal and Brattleboro rat. Amer. J. Anat. 140, 583–588 (1974)

  17. Monroe, B.G.: A comparative study of the ultrastructure of the median eminence, infundibular stem and neural lobe of the hypophysis of the rat. Z. Zellforsch. 76, 405–432 (1966)

  18. Nakai, Y.: On different types of nerve endings in the frog median eminence after fixation with permanganate and glutaraldehyde-osmium. Z. Zellforsch. 119, 164–178 (1971)

  19. Palay, S.L.: The fine structure of the neurohypophysis. In: Progress in neurobiology (eds., Korey, S.R. and J.I. Nurnberger), Vol. II, p. 31–49. New York: P. Hoeber 1957

  20. Pelletier, G., Leclerc, R., Labrie, F., Puriani, R.: Electron microscope immunohistochemical localization of neurophysin in the rat hypothalamus and pituitary. Mol. Cell. Endocrinol. 1, 157 (1974)

  21. Porte, A., Klein, M.-J., Stoeckel, M.E., Stutinsky, M.F.: Sur la conservation ultrastructurale du matérial sécrétaire de la neurohypophyse et de l'hypophyse intermédiaire en fonction des techniques de fixation et de “coloration”. C.R. Acad. Sci. (Paris) 267, 1051–1053 (1968)

  22. Reynolds, E.S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)

  23. Rodríguez, E.M.: Ultrastructure of the neurohaemal region of the toad median eminence. Z. Zellforsch. 93, 182–212 (1969)

  24. Rodríguez, E.M.: Comparative and functional morphology of the median eminence. In: Brain-endocrine interaction. Median eminence: Structure and function (K.M. Knigge, D.E. Scott, A. Weindl, eds.), p. 319–334. Basel: S. Karger 1972

  25. Sterba, G.: Ascending neurosecretory pathway of the peptidergic type. In: Neurosecretion—the final neuroendocrine pathway (eds., F. Knowles and L. Vollrath), p. 38–47. Berlin-Heidelberg-New York: Springer 1974

  26. Sterba, G.: Das oxytocinerge neurosekretorische System der Wirbeltiere. Beitrag zu einem erweiterten Konzept. Zool. Jb. Physiol. 78, 409–423 (1974b)

  27. Wegelin, R., Sterba, G., Hoheisel, G.: Licht- und elektronenmikroskopische Untersuchungen am exohypothalamischen oxytocinergen System von Pleurodeles waltli Michah. (Urodela). (in press) (1975)

  28. Wohlfarth-Bottermann, K.E.: Die Kontrastierung tierischer Zellen und Gewebe im Rahmen ihrer elektronenmikroskopischen Untersuchungen an ultradünnen Schnitten. Naturwissenschaften 44, 287–288 (1957)

Download references

Author information

Correspondence to Prof. Dr. sc. Dr. h.c. G. Sterba.

Additional information

Dedicated to Professor Dr. Dres h.c. W. Bargmann on the occasion of his 70th birthday.

Granted by the Ministerium für Wissenschaft und Technik der DDR.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Naumann, W., Sterba, G. Ultrastructural studies on neurophysine-containing vesicles of the neurosecretory system of vertebrates. Cell Tissue Res. 165, 545–553 (1976). https://doi.org/10.1007/BF00224479

Download citation

Key words

  • Neurophysine-containing vesicles
  • Neurosecretory system
  • Vertebrates
  • Electron microscopy