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Detection of endo-xylanase activities in electrophoretic gels with congo red staining

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Summary

A modification of the Congo Red zymographic technique is used to analyse multiple xylanase activities of Bacillus sp. MIR-32 after PAGE and SDS-PAGE. Xylanase activities are associated with at least two major proteins of 18.4 and 19.6 kDa.

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Breccia, J.D., Baigorf, M.D., Castro, G.R. et al. Detection of endo-xylanase activities in electrophoretic gels with congo red staining. Biotechnol Tech 9, 145–148 (1995). https://doi.org/10.1007/BF00224415

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Keywords

  • Bacillus
  • Congo
  • Xylanase Activity
  • Major Protein
  • Multiple Xylanase