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Conservation of the organization of the streptokinase gene region among pathogenic streptococci

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Abstract

Using ten gene-specific probes from the cloned and sequenced streptokinase gene (skc) region (8 931 bp) of Streptococcus equisimilis H46A, a human serogroup C strain, the conservation of these genes and their linkage relationships were studied by Southern hybridization in pathogenic streptococci differing taxonomically, serologically, in regard to their host range, and in the class of plasminogen activator produced. The results indicate that in S. pyogenes (strains A374, NZ131 and SF130/13) and a human group G strain (G19 908) both gene content and gene order as determined for H46A (dexB-abc-lrp-skc-orf1-rel) are preserved. The same is true of an equine S, equisimilis isolate (87-542-W), the streptokinase gene of which has been shown to hybridize detectably with skc, a result at variance with that obtained previously by others. In contrast, the chromosomal DNA of three S. uberis strains (0140J, C198, C216) of bovine origin, two of which produced a plasminogen activator different from streptokinase, hybridized only with dexB-, abc- and rel-specific probes, and the homologues of these genes appeared to lie close to each other. The maintenance of the organization of the streptokinase gene region in strains differing in overall chromosomal character suggests that this gene arrangement is of selective advantage.

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References

  1. 1.

    Caparon MG, Scott JR (1991) Genetic manipulation of pathogenic streptococci. Methods Enzymol 204:556–586

  2. 2.

    Cashel M, Rudd KE (1987) The stringent response. In: Neidhardt FC (ed) Escherichia coli and Salmonella typhimurium. American Society for Microbiology, Washington, DC, pp 1410–1438

  3. 3.

    Estrada MP, Hernandez L, Perez A, Rodriguez P, Serrano R, Rubiera R, Pedraza A, Padron G, Antuch W, de la Fuente J, Herrera L (1992) High level expression of streptokinase in Escherichia coli. Biotechnology 10:1138–1142

  4. 4.

    Gase K, Ellinger T, Malke H (1995) Complex transcriptional control of the streptokinase gene of Streptococcus equisimilis H46A. Mol Gen Genet 247:749–758

  5. 5.

    Gentry DR, Hernandez VJ, Nguyen LH, Jensen DB, Cashel M (1993) Synthesis of the stationary-phase sigma factor σs is positively regulated by ppGpp. J Bacteriol 175:7982–7989

  6. 6.

    Higgins CF (1992) ABC transporters: from microorganisms to man. Annu Rev Cell Biol 8:67–113

  7. 7.

    Huang TT, Malke H, Ferretti JJ (1989) Heterogeneity of the streptokinase gene in group A streptococci. Infect Immun 57:502–506

  8. 8.

    Huang TT, Malke H, Ferretti JJ (1989) The streptokinase gene of group A streptococci: cloning, expression in Escherichia coli, and sequence analysis. Mol Microbiol 3:197–205

  9. 9.

    Kapur V, Kanjilal S, Hamrick MR, Li L-L, Whittam TS, Sawyer SA, Musser JM (1995) Molecular population genetic analysis of the streptokinase gene of Streptococcus pyogenes: mosaic alleles generated by recombination. Mol Microbiol 16:509–519

  10. 10.

    Leigh JA (1994) Purification of a plasminogen activator from Streptococcus uberis. FEMS Microbiol Lett 118:153–158

  11. 11.

    Malke H, Ferretti JJ (1984) Streptokinase: cloning, expression, and excretion by Escherichia coli. Proc Natl Acad Sci USA 81:3557–3561

  12. 12.

    Malke H, Roe B, Ferretti JJ (1985) Nucleotide sequence of the streptokinase gene from Streptococcus equisimilis H46A. Gene 34:357–362

  13. 13.

    Malke H, Steiner K, Gase K, Mechold U, Ellinger T (1995) The streptokinase gene: allelic variation, genomic environment, and expression control. In: Ferretti JJ, Gilmore MS, Klaenhammer TR, Brown F (eds) Genetics of streptococci, enterococci and lactococci. Karger, Basel, pp 183–193

  14. 14.

    Mechold U, Steiner K, Vettermann S, Malke H (1993) Genetic organization of the streptokinase region of the Streptococcus equisimilis H46A chromosome. Mol Gen Genet 241:129–140

  15. 15.

    Mechold U, Cashel M, Steiner K, Gentry D, Malke H (1994) Functional analysis of the rel gene of Streptococcus equisimilis. 4th International ASM Conference on Streptococcal Genetics, Santa Fe, N.M. American Society for Microbiology, Washington, DC, abstract T8

  16. 16.

    Müller J, Reinert H, Malke H (1989) Streptokinase mutations relieving Escherichia coli K-12 (prlA4) of detriments caused by the wild-type skc gene. J Bacteriol 171:2202–2208

  17. 17.

    Nowicki ST, Minning-Wenz D, Johnston KH, Lottenberg R (1994) Characterization of a novel streptokinase produced by Streptococcus equisimilis of non-human origin. Thromb Haemost 72:595–603

  18. 18.

    Ohkuni H, Todome Y, Suzuki H, Mizuse M, Kotani N, Horiuchi K, Shikama N, Tsugita A, Johnston KH (1992) Immunochemical studies and complete amino acid sequence of the streptokinase from Streptococcus pyogenes (group A) M type 12 strain A374. Infect Immun 60:278–283

  19. 19.

    QIAGEN Plasmid Handbook (1992) QIAGEN GmbH, Hilden, Germany

  20. 20.

    QIAEX Handbook (1993) QIAGEN GmbH, Hilden, Germany

  21. 21.

    Russel RRB, Aduse-Opuku J, Sutcliffe IC, Tao L, Ferretti JJ (1992) A binding protein-dependent transport system in Streptococcus mutans responsible for multiple sugar metabolism. J Biol Chem 267:4631–4637

  22. 22.

    Sambrook J, Fritsch EF, Maniatis TE (eds) (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor

  23. 23.

    Steiner K, Malke H (1995) Transcription termination of the streptokinase gene of Streptococcus equisimilis H46A: bidirectionality and efficiency in homologous and heterologous hosts. Mol Gen Genet 246:374–380

  24. 24.

    Walter F, Siegel M, Malke H (1989) Nucleotide sequence of the streptokinase gene from a Streptococcus pyogenes type 1 strain. Nucleic Acids Res 17:1261

  25. 25.

    Walter F, Siegel M, Malke H (1989) Nucleotide sequence of the streptokinase gene from a group-G Streptococcus. Nucleic Acid Res 17:1262

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Frank, C., Steiner, K. & Malke, H. Conservation of the organization of the streptokinase gene region among pathogenic streptococci. Med Microbiol Immunol 184, 139–146 (1995). https://doi.org/10.1007/BF00224351

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Key words

  • Streptococcus equisimilis
  • S. pyogenes
  • S. uberis
  • Streptokinase gene region
  • Southern hybridization
  • Genome organization