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Transformation of cytoplasmic actin importance for the organization of the contractile gel reticulnm and the contraction — relaxation cycle of cytoplasmic actomyosin

Summary

(1) Within the low viscous flowing endoplasm of Physarum polycephalum a considerable amount of actin is in the non-filamentous state. This can be demonstrated by applying poly-L-lysin to surface spreads of native protoplasm. (2) It has been shown that in protoplasmic drops the endoplasm-ectoplasm transformation is accompanied by an actin polymerization from the non-filamentous state to F-actin. (3) The actual state of the labile G-F-actin equilibrium determines the varying consistency (viscosity) of the cytoplasm. (4) Increasing viscosity can be interpreted as being brought about by a) shifting of the G-F-actin equilibrium to the filamentous side, and (b) increased myosin-mediated binding sites between actin filaments. (5) Polymerization and depolymerization processes are involved in the rhythmically occurring contraction-relaxation cycle of cytoplasmic actomyosin in Physarum. (6) Cytoplasmic actin and myosin represent the architectural proteins of the contractile gel reticulum in eukaryotic cells. (7) The importance of the regulation of actin polymerization as a basic control mechanism of the eukaryotic cell is discussed.

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Correspondence to Prof. Dr. K. E. Wohlfarth-Bottermann.

Additional information

The authors wish to thank Dr. H. Hinssen and R. Beck for the preparation of HMM from rabbit skeletal muscle and for performing the gel-electrophoresis, and Dr. R.L. Snipes for reading the manuscript.

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Isenberg, G., Wohlfarth-Bottermann, K.E. Transformation of cytoplasmic actin importance for the organization of the contractile gel reticulnm and the contraction — relaxation cycle of cytoplasmic actomyosin. Cell Tissue Res. 173, 495–528 (1976). https://doi.org/10.1007/BF00224311

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Key words

  • Cytoplasmic actomyosin
  • Actin transformation
  • Contractile gel reticulum
  • Cytoplasmic viscosity
  • Cell motility
  • Poly-L-lysin
  • Phalloidin