Southern-blot hybridizations of total DNA to mitochondrial DNA (mtDNA) probes were used to investigate the extent of mtDNA variability in cultures derived from immature embryos of diploid (Triticum monococcum, genomic formula: AA, T. tauschii, genomic formula: DD), allotetraploid (T. durum cv “Creso”, genomic formula: AABB), and allohexaploid (T. aestivum, genomic formula: AABBDD) wheat species. Similar distinct changes in mtDNA organization were observed in in vitro cultures of the derived tetraploid and the hexaploid species with related genomes. The tetraploid and hexaploid species share the B genome and mtDNA variability in in vitro culture is known to be under nuclear control. These results suggest that a study of B genome diploids and other polyploid combinations would now shed light on whether or not mtDNA variability in tissue cultures is under B-genome control.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Aubry C, de Buyser J, Hartmann C, Henry Y, Rode A (1989) Changes in the molecular organization of the mitochondrial genome in albino tissue cultures derived from wheat pollen embryos and in plants regenerated from these cultures. Plant Sci 65:103–110
Breiman A (1987) Mitochondrial DNA diversity in the genera of Triticum and Aegilops revealed by Southern-blot hybridization. Theor Appl Genet 73:563–570
Brown PTH, Lange FD, Kranz E, Lörz H (1993) Analysis of single protoplasts and regenerated plants by PCR and RAPD technology. Mol Gen Genet 237:311–317
Escote-Carlson LJ, Gabay-Laughnan S, Laughnan JR (1990) Nuclear genotype affects mitochondrial genome organization of CMSS maize. Mol Gen Genet 223:457–464
Fauron CMR, Havlik M, Brettell RIS (1990) The mitochondrial genome organization of a maize fertile cmsT revenant line is generated through recombination between two sets of repeats. Genetics 124:423–428
Graur D, Bogher M, Breiman A (1989) Restriction endonuclease profiles of mitochondrial DNA and the origin of the B genome of bread wheat, Triticum aestivum. Heredity 62:335–342
Hartmann C, de Buyser J, Henry Y, Falconet D, Lejeune B, Benslimane AA, Quétier F, Rode A (1987) Time-course of mitochondrial genome variation in wheat embryogenic somatic tissue cultures. Plant Sci 53:191–198
Hartmann C, Henry Y, de Buyser J, Aubry C, Rode A (1989) Identification of new mitochondrial genome organizations in wheat plants regenerated from somatic tissue cultures. Theor Appl Genet 77:169–175
Hartmann C, de Buyser J, Henry Y, Morère-Le Paven MC, Dyer TA, Rode A (1992) Nuclear genes control changes in the organization of the mitochondrial genome in tissue cultures derived from immature embryos of wheat. Curr Genet 21:515–520
Hartmann C, Récipon H, Jubier MF, Valon C, Delcher-Besin E, Henry Y, de Buyser J, Lejeune B, Rode A (1994) Mitochondrial DNA variability detected in a single wheat regenerant involves a rare recombination event across a short repeat. Curr Genet 25:456–464
Ketner K, Kelly TJ (1976) Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases. Proc Natl Acad Sci USA 73:1102–1106
Kimber G, Feldman M (1987) In: Wild wheat: an introduction. Special report 353. College of Agriculture, University of Missouri, Columbia, USA, pp 1–4
Kimber G, Sears ER (1987) Evolution in the genus Triticum and the origin of the cultivated wheat. In: Heyne EG (ed) Wheat and wheat improvement. Agronomy monograph no. 13 (2nd edn) Madison, Wisconsin, USA, pp 155–164
Mackenzie SA, Pring DR, Bassett MJ, Chase CD (1988) Mitochondrial DNA rearrangement associated with fertility restoration and cytoplasmic reversion to fertility in cytoplasmic male sterile Phaseolus vulgaris L.. Proc Natl Acad Sci USA 85:2714–2717
Morre-Le Paven MC, Henry Y, de Buyser J, Corre F, Hartmann C, Rode A (1992) Organ/tissue-specific changes in the mitochondrial genome organization of in vitro cultures derived from different expiants of a single wheat variety. Theor Appl Genet 85:1–8
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
Newton KJ, Knudsen C, Gabay-Laughnan S, Laughnan JR (1990) An abnormal growth mutant in maize has a defective mitochondrial cytochrome oxidase gene. Plant Cell 2:107–113
Quétier F, Lejeune B, Delorme S, Falconet D, Jubier MF (1985) Molecular form and function of the wheat mitochondrial genome. In: Groot J, Hall T (eds). Molecular form and function of plant genomes. Plenum Press, New York, pp 413–420
Rode A, Hartmann C, Dron M, Picard E, Quétier F (1985) Organelle genome stability in anther-derived doubled haploids of wheat (Triticum aestivum L., cv ‘Moisson’). Theor Appl Genet 71:320–324
Rode A, Hartmann C, Falconet D, Lejeune B, Quétier F, Benslimane AA, Henry Y, de Buyser J (1987) Extensive mitochondrial DNA variation in somatic tissue cultures initiated from wheat immature embryos. Curr Genet 12:369–376
Rode A, Hartmann C, de Buyser J, Henry Y (1988) Evidence for a direct relationship between mitochondrial genome organization and regeneration ability in hexaploid wheat somatic tissue cultures. Curr Genet 14:387–394
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Vedel F, Quétier F, Dosba F, Doussinault G (1978) Study of wheat phylogeny by EcoRI analysis of chloroplastic and mitochondrial DNAs. Plant Sci Lett 13:97–102
Vedel F, Quétier F, Cauderon Y, Dosba F, Doussinault G (1981) Studies on maternal inheritance in polyploid wheats with cytoplasmic DNAs as genetic markers. Theor Appl Genet 59:239–245
Winfield M, Davey MR, Karp A (1993) A comparison of chromosome instability in cell suspensions of diploid, tetraploid and hexaploid wheats. Heredity 70:187–194
Communicated by J. W. Snape
About this article
Cite this article
Hartmann, C., Winfield, M., Corre, F. et al. A comparative study of the mitochondrial genome organization in in vitro cultures of diploid, tetraploid, and hexaploid Triticum species. Theoret. Appl. Genetics 93, 968–974 (1996). https://doi.org/10.1007/BF00224100
- Mitochondrial DNA
- Somatic tissue culture
- Chondriome variability