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A genome-wide search for wild-species alleles that increase horticultural yield of processing tomatoes


To identify QTLs associated with horticultural yield it is necessary to conduct replicated plot trials of the tested genotypes. The first step in the utilization of an introgression-line (IL) population of Lycopersicon pennellii in a processing-tomato variety (M82) for mapping such QTLs was to screen 51 ILs in a non-replicated plot trial. The results of this survey were compared to those obtained in a replicated trial of the same genotypes grown as single plants at wide spacing. Fruit characteristics were similar between the two stands, but yield was generally different. Eight lines that outperformed the control in the plot survey were subjected to detailed analysis in the following year. The effects of these introgressions, measured on single plants, were reproducible relative to the previous year's results. In a replicated plot trial of these ILs and their hybrids involving two genetic backgrounds, the product of yield and total soluble solids (horticultural yield) in seven of the eight hybrids was 7–13% higher than that of their nearly isogenic controls. The results revealed a consistent trend in the interaction between introgression effects and genetic background. Combining the two introgressions with the largest contribution to horticultural yield in plots resulted in a 20% increase relative to the control in the third year. This research highlights the potential of wild germ plasm for yield improvement and the ability of nearly isogenic populations to achieve this goal.

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  1. Darvasi A, Weinreb A, Minke V, Weller JI, Soller M (1993) Detecting marker — QTL linkage and estimating QTL gene effect and map location using a saturated genetic map. Genetics 134:943–951

  2. De Vicente MC, Tanksley SD (1993) QTL analysis of transgressive segregation in an interspecific tomato cross. Genetics 134:585–596

  3. Doebley J, Stec A, Gustus C (1995) Teosinte branched 1 and the origin of maize: evidence for epistasis and the evolution of dominance. Genetics 141:333–346

  4. Dunnet CW (1955) A multiple comparison procedure for comparing several treatments with a control. J Am Stat Assoc 50:1096–1121

  5. Eshed Y, Zamir D (1994 a) Genomic library of Lycopersicon pennellii in L. esculentum: a tool for fine mapping of genes. Euphytica 79:175–179

  6. Eshed Y, Zamir D (1994 b) Introgressions from Lycopersicon pennellii can improve the soluble solids yield of tomato hybrids. Theor Appl Genet 88:891–897

  7. Eshed Y, Zamir D (1995) An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTLs. Genetics 141:1147–1162

  8. Eshed Y, Zamir D (1996) Less than additive epistatic interactions of quantitative trait loci in tomato. Genetics (in press)

  9. Eshed Y, Abu-Abied M, Saranga Y, Zamir D (1992) Lycopersicon esculentum lines containing small overlapping introgressions from L. pennellii. Theor Appl Genet 83:1027–1034

  10. Griffing B (1990) Use of a controlled nutrient experiment to test the heterosis hypothesis. Genetics 126:753–767

  11. Jensen NF (1988) Methods shaped by competitive forces. In: Plant breeding methodology. A Wiley Interscience Publication, New York, pp 63–104

  12. Lark GK, Chase K, Adler F, Mansur LM, Orf JH (1995) Interaction between quantitative trait loci in soybean in which trait variation at one locus is conditional upon a specific allele at another. Proc Natl Acad Sci USA 92:4656–4660

  13. Martineau B, Summerfelt KR, Adams DF, DeVerna JW (1995) Production of high-solids tomatoes through molecular modification of levels of the plant growth regulator cytokinin. Bio/Technology 13:250–254

  14. Paterson AH, DeVerna JW, Lanini B, Tanksley SD (1990) Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes, in an interspecific cross of tomato. Genetics 124:735–742

  15. Powell W, Thomas WTB, Thompson DM, Swanston JS, Waugh R (1992) Association between rDNA alleles and quantitative traits in doubled-haploid populations of barley. Genetics 130:187–194

  16. Rick CM (1974) High soluble-solids content in large-fruited tomato lines derived from a wild green-fruited species. Hilgardia 42:493–510

  17. Sas Institute (1994) JMP Statistics and graphics guide: version 3. SAS Institute Inc., Cary, North Carolina

  18. Severson DA, Rasmusson DC (1968) Performance of barley hybrids at four seeding rates. Crop Sci 8:339–341

  19. Stevens MA, Rick CM (1986) Genetics and breeding. In: The tomato crop, a scientific basis for improvement. Atherton JG, Rudich J (eds). Chapman and Hall, New York, pp35–109

  20. Stuber CW, Lincoln SE, Wolff DE, Helentjaris T, Lander ES (1992) Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers. Genetics 132:823–839

  21. Tanksley SD (1993) Mapping polygenes. Annu Rev Genet 27:205–233

  22. Tanksley SD, Ganal MW, Prince JC, de Vicente MC, Bonierabale MW, Broun P, Fulton TM, Giovanonni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Roder MS, Wing RA, Wu W, Young ND (1992) High density molecular linkage maps of the tomato and potato genomes: biological inferences and practical applications. Genetics 132:1141–1160

  23. Tanksley SD, Grandillo S, Fulton TM, Zamir D, Eshed Y, Petiard V, Lopez J, Beck-Bunn T (1996) Advanced backcross QTL analysis in a cross between an elite processing line of tomato and its wild relative L. pimpinellifolium. Theor Appl Genet 92:213–224

  24. Xiao J, Li J, Yuan L, Tanksley SD (1995) Dominance is the major genetic basis of heterosis in rice as revealed by QTL analysis using molecular markers. Genetics 140:745–754

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Communicated by F. Salamini

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Eshed, Y., Gera, G. & Zamir, D. A genome-wide search for wild-species alleles that increase horticultural yield of processing tomatoes. Theoret. Appl. Genetics 93, 877–886 (1996). https://doi.org/10.1007/BF00224089

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Key words

  • Breeding
  • QTL
  • Wild germ plasm
  • Introgression lines
  • Lycopersicon pennellii