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Comparison of crossability, RAPD, SDS-PAGE and morphological markers for revealing genetic relationships within and among Lens species

Abstract

The phylogenetic relationships among (sub)-species in the genus Lens have been reviewed based on recent published reports. There was both a substantial level of agreement and disagreement between reports based on different analytical procedures and different plant germ plasms. Lens culinaris ssp. orientalis appeared as the wild progenitor of the cultivated lentils. A gene flow from L. odemensis and L. ervoides during lentil crop evolution was suggested. Morphological characters (quantitative and qualitative) showed a different taxonomic pattern in the genus Lens. The use of nuclear and biochemical markers (RFLPs, RAPDs, seed-protein electrophoresis) appeared to be the most consistent and reliable methods for determining genetic relationships. It is suggested that these techniques be used in combination for taxonomic analysis of the genus Lens.

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References

  1. Abo-elwafa A, Murai K, Shimada T (1995) Intra- and inter-specific variation in Lens species revealed by RAPD markers. Theor Appl Genet 90:335–340

  2. Ahmad M, Fautrier AG, Burritt DJ (1955 a) Genetic diversity and relationships in Lens species and their interspecific hybrids as determined by SDS-PAGE. Plant Breed (in press)

  3. Ahmad M, Fautrier AG, McNeil DL, Burritt DJ, Hill GD (1995 b) Attempts to overcome post-fertilization barriers in interspecific crosses in the genus Lens. Plant Breed 114:558–560

  4. Ahmad M, McNeil DL, Sedcole JR (1995 c) Phylogenetic relationships in Lens species and their interspecific hybrids as measured by morphological characters. Euphytica (in press)

  5. Ahmad M, McNeil DL, Fautrier AG, Armstrong KF, Paterson AM (1996) Genetic relationships in Lens species and parentage determination of their interspecific hybrids using RAPD markers. Theor Appl Genet 92:1091–1098

  6. Barulina H (1930) Lentils of the USSR and other countries. Trudy Prikl Bot Selekc Suppl 40:1–319

  7. Havey MJ, Muehlbauer FJ (1989) Variability for restriction fragment lengths and phylogenesis in lentil. Theor Appl Genet 77:839–843

  8. Hoffman DL, Soltis DE, Muehlbauer FJ, Ladizinsky G (1986) Isozyme polymorphism in Lens (Leguminosae). Systematic Bot 11:392–402

  9. Hoffman DL, Muehlbauer FJ, Ladizinsky G (1988) Morphological variation in Lens (Leguminosae). Systematic Bot 13:87–96

  10. Ladizinsky G (1979 a) The origin of lentil and its wild gene pool. Euphytica 28:179–187

  11. Ladizinsky G (1979 b) Species relationships in the genus Lens as indicated by seed-protein electrophoresis. Bot Gaz 140:449–451

  12. Ladizinsky G (1993) Wild lentils. Crit Rev Plant Sci 12(3):169–184

  13. Ladizinsky G, Braun D, Goshen D, Muehlbauer FJ (1984) The biological species of the genus Lens. Bot Gaz 145:253–261

  14. Liu Z, Furnier GR (1993) Comparison of allozyme, RFLP, and RAPD markers for revealing genetic variation within and between trembling aspen and bigtooth aspen. Theor Appl Genet 87:97–105

  15. MacKey J (1988) A plant breeder's aspect on the taxonomy of cultivated plants. Biol Zentral B1 107:369–379

  16. Mayer MS, Soltis PS (1994) Chloroplast DNA phylogeny of Lens (Leguminosae): origin and diversity of the cultivated lentil. Theor Appl Genet 87:773–781

  17. Muench DG, Slinkard AE, Scoles GJ (1991) Determination of genetic variation and taxonomy in lentil (Lens Miller) species by chloroplast DNA polymorphism. Euphytica 56:213–218

  18. Nienhuis J, Sills G (1992) The potential of hybrid varieties in selfpollinated vegetables. In:Dattee Y, Dumas C, Gallais A (eds) Reproductive biology and plant breeding. Springer-Verlag, pp 387–396

  19. Pinkas R, Zamir D, Ladizinsky G (1985) Allozyme divergence and evolution in the genus Lens. Pl Syst Evol 151:131–140

  20. Rajora OP, Mahon JD (1994) Inheritance of mitochondrial DNA in lentil (Lens culinaris Medik.). Theor Appl Genet 89:206–210

  21. Rajora OP, Mahon JD (1995) Paternal plastid DNA can be inherited in lentil. Theor Appl Genet 90:607–610

  22. Rosa L, Jouve N (1992) Genetic variation for isozyme genes and proteins in Spanish primitive cultivars and wild species of Lens. Euphytica 59:181–187

  23. Sammour RH (1994) Species relationships in the genus Lens as indicated by electrophoresis — a reappraisal. Lens Newslett 21:1–4

  24. Sharma SK, Dawson IK, Waugh R (1995) Relationships among cultivated and wild lentils revealed by RAPD analysis. Theor Appl Genet 91:647–654

  25. Smith OS, Smith JSC, Browen SL, Tenborg RA, Wall SJ (1990) Similarities among a group of elite maize inbreds as measured by pedigree, F1 grain yield, grain yield, heterosis, and RFLPs. Theor Appl Genet 80:387–396

  26. Williams JT, Sanchez AMC, Jackson MT (1974) Studies on lentils and their variation. 1. The taxonomy of the species. Soc Adv Breeding Res in Asia and Oceania J 6:133–145

  27. Zohary D (1972) The wild progenitor and the place of origin of the cultivated lentil Lens culinaris. Econ Bot 26:326–332

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Communicated by J. MacKey

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Ahmad, M., McNeil, D.L. Comparison of crossability, RAPD, SDS-PAGE and morphological markers for revealing genetic relationships within and among Lens species. Theoret. Appl. Genetics 93, 788–793 (1996). https://doi.org/10.1007/BF00224077

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Key words

  • RAPD
  • SDS-PAGE
  • Morphological markers
  • Crossability
  • Genetic relationships
  • Genus Lens