Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Mapping of β-glucan content and β-glucanase activity loci in barley grain and malt

  • 384 Accesses

  • 102 Citations

Abstract

Genetic study of β-glucan content and β-glucanase activity has been facilitated by recent developments in quantitative trait loci (QTL) analysis. QTL for barley and malt β-glucan content and for green and finished malt β-glucanase activity were mapped using a 123-point molecular marker linkage map from the cross of Steptoe/Morex. Three QTL for barley β-glucan, 6 QTL for malt β-glucan, 3 QTL for β-glucanase in green malt and 5 QTL for β-glucanase in finished malt were detected by interval mapping procedures. The QTL with the largest effects on barley β-glucan, malt βglucan, green malt β-glucanase and finished malt βglucanase were identified on chromosomes 2,1,4 and 7, respectively. A genome map-based approach allows for dissection of relationships among barley and malt βglucan content, green and finished malt β-glucanase activity, and other malting quality parameters.

This is a preview of subscription content, log in to check access.

References

  1. Bamforth CW, Barclay AHP (1993) Malting technology and the uses of malt. In: MacGregor AW, Bhatty RS (eds) Barley: chemistry and technology. Am Assoc Cereal Chem, St. Paul, Minn., pp 297–354

  2. Bamforth CW, Martin HL (1981) β-glucan and β-glucan solubilase in malting and mashing. J Inst Brew 87:365–372

  3. Chen F, Hayes PM (1989) A comparison of Hordeum bulbosum mediated haploid production efficiency in barley using in vitro floret and tiller culture. Theor Appl Genet 77:701–704

  4. Fincher GB (1992) Cell wall metabolism in barley. In: Shewry PR (ed) Barley: Genetics, biochemistry, molecular biology and biotechnology CAB Int, The Alden Press, Oxford, pp 413–437

  5. Hayes PM, Iyambo O (1994) Summary of QTL effects in the Steptoe x Morex population. Barley Genet Newsl 23:98–133

  6. Hayes PM, Liu BH, Knapp SJ, Chen F, Jones B, Blake T, Franc-kowiak J, Rasmusson D, Sorrells M, Ullrich S, Wesenberg D, Kleinhofs A (1993) Quantitative trait locus effects and environmental interaction in a sample of North American barley germ plasm. Theor Appl Genet 87:392–401

  7. Jørgersen KG, Aastrup S (1988) Determination of β-glucan in barley, malt, wort and beer. In: Linskens HF, Jackson JF (eds) Modern methods of plant analysis, new series: beer analysis, vol 12. Springer, Berlin Heidelberg New York, pp 88–108

  8. Kleinhofs A, Kilian A, Saghai Maroof MA, Biyashev RM, Hayes P, Chen FQ, Lapitan N, Fenwich A, Blake TK, Kanazin V, Ananiev E, Dahleen L, Kudrna D, Bollinger J, Knapp SJ, Liu B, Sorrells M, Heun M, Franckowiak JD, Hoffman D, Skadsen R, Steffenson BJ (1993) A molecular, isoenzyme and morphological map of the barley (Hordeum vulgare) genome. Theor Appl Genet 86:705–712

  9. Kleinhofs A, Kilian A, Kudrna D (1994) The NABGMP Steptoe x Morex mapping progress report. Barley Genet Newsl 23:79–83

  10. Kreis M, Shewry PR (1992) The control of protein synthesis in developing barley seeds. In: Shewry PR (ed) Barley: genetics, biochemistry, molecular biology and biotechnology. CAB Int, The Alden Press, Oxford, pp 319–334

  11. Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121: 185–199

  12. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

  13. Lincoln SE, Daly M, Lander ES (1992) Mapping genes controlling quantitative traits with MAPMAKER/QTL 1.1. Tech Rep 2nd edn. Whitehead Institute for Biomedical Research, Cambridge, Mass.

  14. Liu BH, Knapp SJ (1990) GMENDEL: A program for Mendelian segregation and linkage analysis of individual or multiple progeny populations using log-likelihood ratios. J Hered 81:407

  15. Loi L, Ahluwalia B, Fincher GB (1988) Chromosomal location of genes encoding barley (1–3, 1–4)-β-glucan 4-glucanohydrolases. Plant Physiol 87:300–302

  16. Martin HL, Bamforth CW (1980) The relationship between β-glucan solubilase, barley autolysis and malting potential. J Inst Brew 86:216–221

  17. McCleary BV, Shameer I (1987) Assay of malt β-glucanase using Azo-barley glucan: an improved precipitant. J Inst Brew 93:87–90

  18. Newman RK, Newman LW, Graham H (1989) The hypocholesterolemic function of barley β-glucans. Cereal Foods World 34:883–886

  19. Petersen VE (1972) The properties and values of the various feed grains in poultry nutrition. In: Cereal processing and digestion. US Feed Grains Council, London, pp 67–75

  20. Powell W, Caligari PDS, Swanston JS, Jinks JL (1989) Genetical investigations into beta-glucan content in barley. Theor Appl Genet 71:461–466

  21. Slakeski N, Baulcombe DC, Devos KM, Ahluwalia B, Doan DNP, Fincher GB (1990) Structure and tissue-specific regulation of genes encoding barley (1–3, l–4)-β-glucan endohydrolases. Mol Gen Genet 224:437–439

  22. Stuart IM, Loi L, Fincher GB (1988) Varietal and environmental variations in (1–3, 1–4)-β-glucan levels and (1–3, 1–4)-β-glucanase potential in barley: relationships to malting quality. J Cereal Sci 7:61–71

  23. Ullrich SE, Hayes PM, Dyer WE, Blake TK, Clancy JA (1993) Quantitative trait locus analysis of seed dormancy in “Steptoe” barley. In: Walker-Simmons MK, Ried JL (eds) Pre-harvest sprouting in cereals 1992. Am Assoc Cereal Chem, St Paul, Minn., pp 136–145

  24. van Ooijen JW (1992) Accuracy of mapping quantitative trait loci in autogamous species. Theor Appl Genet 84:803–811

  25. Woodward JR, Fincher GB (1982a) Purification and chemical properties of two (1–3, 1–4)-β-d-glucan endohydrolases from germinating barley. Eur J Biochem 121:663–669

  26. Woodward JR, Fincher GB (1982b) Substrate specificities and kinetic properties of two (1–3, 1–4)-β-d-glucan endohydrolases from germinating barley (Hordeum vulgare). Carbohydr Res 106:111–122

Download references

Author information

Additional information

Communicated by G. Wenzel

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Han, F., Ullrich, S.E., Chirat, S. et al. Mapping of β-glucan content and β-glucanase activity loci in barley grain and malt. Theoret. Appl. Genetics 91, 921–927 (1995). https://doi.org/10.1007/BF00223901

Download citation

Key words

  • QTL mapping
  • β-Glucan
  • β-Glucanase Malt barley
  • Hordeum vulgare