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Organization of nuclear ribosomal DNA and species-specific polymorphism in closely related Fraxinus excelsior and F. oxyphylla

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Abstract

The ribosomal DNA repeat units of two closely related species of the genus Fraxinus, F. excelsior and F. oxyphylla, were characterized. The physical maps were constructed from DNA digested with BamHI, EcoRI, EcoRV and SacI, and hybridized with three heterologous probes. The presence or the absence of an EcoRV restriction site in the 18s RNA gene characterizes two ribosomal DNA unit types found in both species and which coexist in all individuals. A third unit type appeared unique to all individuals of F. oxyphylla. It carries an EcoRI site in the intergenic spacer. Each type of unit displayed length variations. The rDNA unit length of F. excelsior and F. oxyphylla was determined with EcoRV restriction. It varied between 11kb and 14.5kb in F. excelsior and between 11.8kb to 13.8kb in F. oxyphylla. Using SacI restriction, at least ten spacer length variants were observed in F. excelsior, for which a detailed analysis was conducted. Each individual carries 2–4 length variants which vary by a 0.3-kb step multiple. This length variation was assigned to the intergenic spacer. By using the entire rDNA unit of flax as probe in combination with EcoRI restriction, each species can be unambiguously discriminated. The species-specific banding pattern was used to compare trees from a zone of sympatry between the two species. In some cases, a conflicting classification was obtained from morphological analysis and the use of the species-specific rDNA polymorphism. Implications for the genetic management of both species are discussed.

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References

  1. Appels R, Dvorak J (1982) The wheat ribosomal DNA spacer region: its structure and variation in populations and among species. Theor Appl Genet 63:337–348

  2. Baldridge GD, Dalton MW, Fallon AM (1992) Is higher-order structure conserved in eukaryotic ribosomal DNA intergenic spacers? J Mol Evol 35:514–523

  3. Barker RF, Harberd NP, Jarvis MG, Flavell RB (1988) Structure and evolution of the intergenic region in a ribosomal DNA unit of wheat Plant Mol Biol 201:1–17

  4. Bellarosa R, Delre V, Schirone B, Maggini F (1990) Ribosomal RNA genes in Quercus spp. (Fagaceae). Plant Syst Evol 172:127–139

  5. Besse P, Seguin M, Lebrun P, Lanaud C (1993) Ribosomal DNA variations in wild and cultivated rubber tree (Hevea brasiliensis). Genome 36:1049–1057

  6. Bobola MS, Smith DE, Klein AS (1992a) Five major nuclear ribosomal repeats represent a large and variable fraction of the genomic DNA of Picea rubens and P. mariana. Mol Biol Evol 91:125–137

  7. Bobola MS, Eckert RT, Klein AS (1992b) Restriction-fragment variation in the nuclear ribosomal DNA repeat unit within and between Picea rubens and Picea mariana. Can J For Res 22:255–263

  8. Bousquet J, Girouard E, Strobeck C, Dancik BP, Lalonde M (1989) Restriction-fragment length polymorphisms in the rDNA region among seven species of Alnus and Betula papyrifera. Plant Soil 118:231–240

  9. Burt A, Carter DA, White TJ, Taylor JW (1994) DNA sequencing with arbitrary primers. Mol Ecol 3:523–525

  10. Delseny M, McGrath JM, This P, Chevre AM, Quiros CF (1990) Ribosomal RNA genes in diploid and amphidiploid Brassica and related species:organization, polymorphism and evolution. Genome 33:733–744

  11. Faivre-Rampant P, Jeandroz S, Lefèvre F, Lemoine M, Villar M, Bervillé A (1992a) Ribosomal DNA studies in poplars. Genome 35:733–740

  12. Faivre-Rampant P, Bodergat R, Bervillé A (1992b) Une méthode de classemment des clones de peuplier (Populus) dans les sections Tacamahaca, Aigeiros, Leuce et Leucoides par des fragments de restriction des unités ribosomiques. CR Acad Sci Paris 35:133–138

  13. Gerlach WL, Bedbrook JR (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7:1869–1885

  14. Goldsborough PB, Cullis CA (1981) Characterization of the genes for ribosomal RNA in flax. Nucleic Acids Res 9:1301–1309

  15. Jorgensen RA, Cluster PD (1988) Modes and tempos in the evolution of nuclear ribosomal DNA:new characters for evolutionary studies and new markers for genetic and populations studies. Ann Missouri Bot Gard 75:1238–1247

  16. Kim WK, Innes RL, Kerber ER (1992) Ribosomal DNA repeat unit polymorphism in six Aegilops species. Genome 35:510–515

  17. Lanaud C, Tezenas du Montcel H, Jolivot MP, Glaszman JC, Gonzales de Leon D (1992) Variation of ribosomal gene spacer length among wild and cultivated banana. Heredity 68: 147–156

  18. Levesque H, Vedel F, Mathieu C, De Courcel AGL (1990) Identification of a short rDNA spacer sequence highly specific of a tomato line containing the Tm-1 gene introgressed from Lycopersicon hirsutum. Theor Appl Genet 80:602–608

  19. Morgante M, Olivieri AM (1993) PCR-amplified microsatellites as markers in plant genetics. Plant J 3:175–182

  20. Paran I, Michelmore RW (1993) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85:985–993

  21. Perron M, Gordon AG, Bousquet J (1995) Development of speciesspecific RAPD fingerprints for the black spruce-red spruce complex. Theor Appl Genet (in press)

  22. Poinsot H (1972) Flore de Bourgogne. Librairie de l'Université, Dijon, France

  23. Polans NO, Weeden NF, Thomson WF (1986) Distribution, inheritance and linkage relationships of ribosomal DNA spacer length variants in pea. Theor Appl Genet 72:289–295

  24. Rogers SO, Bendich AJ (1987) Ribosomal DNA genes in plants: variability in copy number and in the intergenic spacer. Plant Mol Biol 9:509–520

  25. Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer length polymorphisms in barley. Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 8:8014–8018

  26. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning:a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

  27. Sano Y, Sano R (1989) Variation of the intergenic spacer region of ribosomal DNA in cultivated and wild rice species. Genome 33:209–218

  28. Santoni S, Bervillé A (1992) Characterization of the nuclear ribosomal DNA units and phylogeny of Beta L. wild forms and cultivated beets. Theor Appl Genet 83:533–542

  29. Savard L, Michaud M, Bousquet J (1993) Genetic diversity and phylogenetic relationships between birches and alders using ITS, 18s, and rbcL gene sequences. Mol Phyl Evol 2:112–118

  30. Schaal BA, Learn GH (1988) Ribosomal DNA variation within and among plant populations. Ann Missouri Bot Gard 75:1207–1216

  31. Smith DN, Devey ME (1994) Occurrence and inheritance of microsatellites in Pinus radiata. Genome 37:977–983

  32. Smith RL, Sytsma RJ (1990) Evolution of Populus nigra (sect. Aigeiros): introgressive hybridization and the cloroplast contribution of Populus alba (sect. Populus). Am J Bot 77:1176–1187

  33. Tremousaygue D, Grellet F, Delseny M, Delourme R, Renard M (1988) The large spacer of a nuclear ribosomal RNA gene from radish: organization and use as a probe in rapeseed breeding. Theor Appl Genet 75:298–304

  34. White TJ, Bruns T, Lee S, Taylor S (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In:Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols. A guide to methods and applications. Academic Press, San Diego, pp 482

  35. Zhang Q, Saghai-Maroof MA, Yang PG (1992) Ribosomal DNA polymorphisms and the Oriental-Occidental genetic differentiation in cultivated barley. Theor Appl Genet 84:682–687

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Communicated by P. M. A. Tigerstedt

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Jeandroz, G., Faivre-Rampant, F., Pugin, A. et al. Organization of nuclear ribosomal DNA and species-specific polymorphism in closely related Fraxinus excelsior and F. oxyphylla . Theoret. Appl. Genetics 91, 885–892 (1995). https://doi.org/10.1007/BF00223896

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Key words

  • Fraxinus excelsior
  • Fraxinus oxyphylla
  • Nuclear rDNA
  • Intergenic spacer variation
  • Interspecific hybridization