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Molecular taxonomic relationships in the genus Vigna based on RFLP analysis


The taxonomy of the genus Vigna has been primarily based on morphological attributes. We have used 27 genomic clones from soybean, common bean, mungbean and cowpea to examine restriction fragment length polymorphism (RFLP) among 44 accessions of different species belonging to four subgenera of the genus Vigna. One accession each of common bean (Phaseolus vulgaris) and soybean (Glycine max) was included in the study. Total DNA from the various genotypes was digested with one restriction enzyme (Eco RV). Results of a numerical taxonomic analysis showed a high level of genetic variation within the genus with a remarkably higher amount of variation associated with Vigna sp. from Africa relative to those from Asia. The distinctness of the Asiatic grams in subgenus Ceratotropis, cowpea in section Catiang, bambara groundnut (V. subterranean) and members of the subgenus Plectotropis was elucidated by this study. Members of the subgenus Plectotropis were closer in genome homology to those of subgenus Vigna section Catiang than to those of subgenus Ceratotropis. The relative positions of some genotypes to one another on the dendrogram and minimum spanning tree were discussed in regard to hybridisations aimed generating well-saturated genomic maps and interspecies transfer of desirable genes.

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  1. Ahn CS, Hartmann RW (1978) Interspecific hybridization between rice bean [Vigna umbellata (Thumb.) Ohwi and Ohashi] and adzukibean [V. angularis (Willd.) Ohwi and Ohashi]. J Am Soc Hortic Sci 103:435–438

  2. Baudoin JP, Marechal R (1985) Genetic diversity in Vigna. In: Singh SR, Rachie KO (eds) Cowpea research, production and utilisation. John Wiley, New York, pp 3–9

  3. Baudoin JP, Marechal R (1988) Taxonomy and evolution of the genus Vigna. In: Mungbean: Proc 2nd Int Symp Asian Vegetable Res Dev Center. Shanhua, Taiwan pp 2–12

  4. Dana S(1980) Genomic relationship in the genus Vigna and its implications in breeding programme. In: Gill KS (ed) Breeding methods for the improvement of pulse crops. Punjab Agric Univ, Ludhiana, pp 357–367

  5. Dana S, Karmakar PG (1990) Species relation in Vigna subgenus Ceratotropis and its implications in breeding. In: Janick J (ed) Plant breeding review. Timber Press, Portland, Or., pp 19–42

  6. Debener T, Salamini F, Gebhardt C (1990) Phylogeny of wild and cultivated Solanum species based on nuclear restriction fragment length polymorphisms (RFLPs). Theor Appl Genet 79:360–368

  7. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA mini-preparation: version II. Plant Mol Biol Rep 1:19–21

  8. D'Urzo MP, Pedalino M, Grillo S, Rao R, Tucci M (1990) Variability in major seed proteins in different Vigna species. In: Ng NQ, Monti LM (eds) Cowpea genetic resources. International Institute of Tropical Agriculture (IITA) Ibadan, Nigeria, pp 90–100

  9. Fatokun CA (1991) Wide hybridization in cowpea: problems and prospects. Euphytica 54:137–140

  10. Fatokun CA, Singh BB (1987) Interspecific hybridization between Vigna pubescens and V. unguiculata through embryo rescue. Plant Cell Tissue Organ Cult 9:229–233

  11. Fatokun CA, Menancio-Hautea DI, Danesh D, Young ND (1992) Evidence for orthologous seed weight genes in cowpea and mungbean based on RFLP mapping. Genetics 132:841–846

  12. Jaaska V, Jaaska V (1986) Isoenzyme variation in the genera Phaseolus and Vigna (Fabaceae) in relation to their systematics: aspartate aminotransferase and superoxide dismutase. Plant Syst Evol 159:145–159

  13. Kochert G, Halward T, Branch WD, Simpson CE (1991) RFLP variability in peanut (Arachis hypogea L.) cultivars and wild species. Theor Appl Genet 81:565–570

  14. Lukoki L, Marechal R, Otoul E (1980) The wild ancestors of the cultivated beans Vigna radiata and V. mungo. Bull Jard Bot Nat Belg 28:23–30

  15. Marechal R, Mascherpa JM, Stainier F (1978) Etude taxonomique d'un groupe complexe d'especes des genres Phaseolus et Vigna (Papillionaceae) sur la base de donnees morphologiques, et polliniques traitees par l'analyse informatique. Boissiera 28:1–273

  16. Marechal R, Mascherpa JM, Stainier F (1981) Taxonometric study of the Phaseolus-Vigna complex and related genera In: Polhill RM, Raven PH (eds) Advances in legume systematics. Proc Legume Conf, Kew, UK, pp 329–335

  17. Miller JC, Tanksley SD (1990) RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon. Theor Appl Genet 80:437–448

  18. Palmer JD, Osorio B, Thompson WF (1988) Evolutionary significance of inversions in legume chloroplast DNAs. Curr Genet 14:65–74

  19. Purseglove JW (1968) Tropical crops. Dicotyledons. Longmans, London

  20. Rohlf FJ (1990) NTSYS-pc. Numerical taxonomy and multivariate analysis system: version 1.60. Applied Biostatistics, New York

  21. Smartt J (1985) Evolution of grain legumes. III. Pulses in the genus Vigna. Exp Agric 21:87–100

  22. Smartt J (1990) Grain legumes: evolution and genetic resources. Cambridge University Press, Cambridge, pp 140–175

  23. Song KM, Osborn TC, Williams PH (1988) Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLP). 1. Genome evolution of diploid and amphidiploid species. Theor Appl Genet 75:784–794

  24. Southern EM (1976) Detection of specific sequences among DNA fragments separated by gel electophoresis. J Mol Biol 98:503–517

  25. Verdcourt B (1970) Studies in the Leguminosae-Papillionoideae for the ‘Flora of tropical East Africa’. IV. Kew Bull 24:507–570

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Correspondence to N. D. Young.

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Communicated by G. Wenzel

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Fatokun, C.A., Danesh, D., Young, N.D. et al. Molecular taxonomic relationships in the genus Vigna based on RFLP analysis. Theoret. Appl. Genetics 86, 97–104 (1993).

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Key words

  • Vigna
  • Numerical taxonomy
  • RFLP
  • Asiatic grams
  • Cowpea
  • Bambara groundnut