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Gynogenic lines of onion (Allium cepa L.): evidence of their homozygosity

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Abstract

Haploid induction via gynogenesis offers the possibility of using doubled haploid (DH) inbred lines in onion breeding. A first DH line that originated from the open-pollinated (OP) cultivar ‘Dorata di Parma’ was obtained after overcoming difficulties associated with the haploidy of the regenerants. Spontaneous chromosome doubling occurs seldom in onion. The first DH line obtained was cloned and selfed to produce sufficient seeds for genetic studies. The homozygosity of the DH gynogenic line was revealed on the basis of the low standard deviations of the bulb traits polar diameter, shape index and weight with respect to those of the S1 line or the OP cultivar. In the DH line, moreover, segregation of RAPD and alpha esterase markers was not noted. Out of four primers revealing polymorphism at 16 ge-netic loci in the OP cultivar ‘Dorata di Parma’, none produced polymorphism in the DH gynogenic line. The Est-1 locus, homozygous in 22 plants (Est-1 1/1 in 3 and Est-1 2/2 in 19) and heterozygous (Est-1 1/2) in 11 plants of the OP cultivar, always carried the same alleles in the DH line. We also tested genetic stability during micropropagation of a second halpoid line obtained via gynogenesis from var. ‘Senshyu Yellow’. Seventeen plants of this line were tested to detect changes occurring during the tissue culture process. Again no polymorphism was observed. The high genetic homogeneity observed in the two gynogenic lines of onion could be related to the absence of the callus phase during the gynogenic process.

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References

  1. Brown PTH, Lange FD, Kranz E, Lörz H (1993) Analysis of single protoplasts and regenerated plants by PCR and RAPD technology. Mol Gen Genet 237:311–317

  2. Campion B, Alloni C (1990) Induction of haploid plants in onion (Allium cepa L.) by in vitro culture of unpollinated ovules. Plant Cell Tissue Org Cult 20:1–6

  3. Campion B, Azzimonti MT (1988) Evolution of ploidy level in haploid plants of onion (Allium cepa L.) obtained through in vitro gynogenesis. In: Riggs TJ, Astley D, Brewster JL, Dawson PR, Maude RB (eds) Proceedings of the Eucarpia 4th Allium Symp. Institute of Horticultural Research, Wellesbourne Warwick, 85–89

  4. Campion B, Azzimonti MT, Vicini E, Schiavi M, Falavigna A (1992) Advances in haploid plant induction in onion (Allium cepa L.) through in vitro gynogenesis. Plant Sci 86:97–104

  5. Campion B, Perri E, Azzimonti MT, Vicini E, Schiavi M (1994) Gynogenic lines of onion (Allium cepa L.): spontaneous and induced chromosome doubling. Plant breed (in press)

  6. Cecchini E, Natali N, Cavallini A, Durante M (1992) DNA variation in regenerated plants of pea (Pisum sativum L.). Theor Appl Genet 84:874–879

  7. D'Amato F (1985) Cytogenetics of plant cell and tissue cultures and their regenerates. In: Conger BV (Ed) CRC Critical Reviews in Plant Sciences, vol 3, Issue 1. CRC Press, Inc. Boca Raton, Fla., pp 73–112

  8. Doré C, Marie F (1993) Production of gynogenetic plants of onion (Allium cepa L.) after crossing with irradiated pollen. Plant Breed 111:142–147

  9. Dowker BD, Fennell JFM (1974) Heritability of bulb shape in some North European onion varieties. Ann Appl Biol 77:61

  10. Evans DA, Sharp WR, Medina-Fihlo HP (1984) Somaclonal and gametoclonal variation. Am J Bot 71:759–774

  11. Finnie SJ, Forster BP, Chalmers KJ, Dyer AF, Waugh R, Powell W (1991) Genetic stability of microspore-derived doubled haploids of barley: a cytological, biochemical, and molecular study. Genome 34:923–928

  12. Jones HA, Davis GN (1944) Inbreeding and heterosis and their relation to the development of new varieties of onions. US Dep Agric Tech Bull 874:1–28

  13. Keller J (1990) Culture of unpollinated ovules, ovaries, and flower buds in some species of the genus Allium and haploid induction via gynogenesis in onion (Allium cepa L.) Euphytica 47:241–247

  14. Kumashiro T, Oinuma T (1985) Comparison of genetic variability among anther-derived and ovule-derived doubled haploid lines of tobacco. Jpn J Breed 35:301–310

  15. Loaiza-Figueroa F, Weeden N (1991) Effects of seed increase procedures on isozyme polymorphism in Allium. FAO/IBPGR Plant Genet Resources Newsl 83/84:1–3

  16. McCollum GD (1971) Heritability of onion bulb shape and size: estimates from half-sib families. J Hered 62:101

  17. Muren R (1989) Haploid plant induction from unpollinated ovaries in onion. HortScience 24:833–834

  18. Murigneux A, Barloy D, Leroy P, Beckert M (1993) Molecular and morphological evaluation of doubled haploid lines in maize. 1. Homogeneity within DH lines. Theor Appl Genet 86:837–842

  19. Oono K (1981) In vitro methods applied to rice. In: Thorpe TA (ed) Plant tissue culture, Academic Press, New York, pp 273–298

  20. Peffley EB, Mangum PD (1990) Introgression of A. fistulosum L. into A. cepa L.: cytogenetic evidence. Theor Appl Genet 79:113–118

  21. Sabir A, Newbury HJ, Todd G, Catty J, Ford-Lloyd BV (1992) Determination of genetic stability using isozymes and RFLPs in beet plants regenerated in vitro. Theor Appl Genet 84:113–117

  22. Saghai Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018.

  23. Singsit C, Ozias-Akins P (1993) Genetic variation in monoploids of diploid potatoes and detection of clone specific random amplified polymorphic DNA markers. Plant Cell Rep 12:144–148

  24. Smith BM, Godwin RM, Harvey E, Werner CP (1991) Gynogenesis from whole flower buds in bulb onions (Allium cepa L.) and leeks (Allium porrum L.). J Genet Breed 45:353–358

  25. Wendel JF, Weeden NF (1989) Visualization and interpretation of plant isozymes. In: Soltis DE, Soltis PS (eds) Isozymes in plant biology. Dioscorius Press, Portland, Ore., pp 5–45

  26. Wernsman E (1992) Varied roles for the haploid sporophyte in plant improvement. In: Stalker HT, Murphy JP (eds) Plant breeding in the 1990s. C.A.B. Int, Wallingforde, UK, pp 461–484

  27. Wilkie S, Isaac P, Slater R (1993) Random amplified polymorphic DNA (RAPD) markers for genetic analysis in Allium. Theor Appl Genet 86:497–504

  28. Ziauddin A, Kasha KJ (1990) Genetic stability in haploid cell cultures. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 12: haploids in crop improvement. Springer, Berlin Heidelberg New York, pp 83–98

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Communicated by Y. Gleba

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Campion, B., Bohanec, B. & Javornik, B. Gynogenic lines of onion (Allium cepa L.): evidence of their homozygosity. Theoret. Appl. Genetics 91, 598–602 (1995). https://doi.org/10.1007/BF00223285

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Key words

  • Gynogenesis
  • Isozyme
  • RAPD
  • Agronomic evaluation
  • Gametoclonal variation
  • Genetic homogeneity