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Chromosomal variation in dividing protoplasts derived from cell suspensions of barley (Hordeum vulgare L.)

Summary

Numerical and structural chromosome variation was analysed in dividing protoplasts isolated from suspension cells of barley. Five cell lines exhibited distribution patterns in chromosome number with different peaks and ranges. Embryogenic/morphogenic cell lines showed a peak at 2n = 14 (ca. 50%) after 6–7 months in culture, while older non-embryogenic cell lines had peaks at aneuploid or polyploid chromosome numbers. Culture duration had a clear effect on numerical and structural chromosome variation in embryogenic cell lines. With ageing of the cultures chromosome variation accumulated and the proportion of 2n = 14 cells decreased. The effect of protoplast isolation and culture on chromosome variation was examined; more cells with normal chromosome sets (12%) were maintained in protoplast-derived colonies than in source suspension cells (4%) of the same culture age.

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Abbreviations

DC :

Dicentric

F :

fragment

T :

telocentric

References

  1. Gaponenko AK, Petrova TF, Igkakov A, Sozinov AA (1988) Cytogenetics of in vitro culture somatic cells and regenerated plants of barley (Hordeum vulgare L). Theor Appl Genet 75:905–911

  2. Goldstein CS, Kronstad WE (1986) Tissue culture and plant regeneration from immature embryo expiants of barley, Hordeum vulgare. Theor Appl Genet 71:631–636

  3. Jähne A, Lazzeri PA, Jäger-Gussen M, Lörz H (1991 a) Plant regeneration from embryogenic cell suspensions derived from anther cultures of barley (Hordeum vulgare L.). Theor Apply Genet 82:74–80

  4. Jähne A, Lazzeri PA, Lörz H (1991 b) Regeneration of fertile plants from protoplasts derived from embryogenic cell suspensions of barley (Hordeum vulgare L.). Plant Cell Rep 10:1–6

  5. Jelaska S, Rengel Z, Cesar V (1984) Plant regeneration from mesocotyl callus of Hordeum vulgare L. Plant Cell Rep 3:125–129

  6. Kao KN (1975) A nuclear staining method for plant protoplasts. In: Gamborg OL, Wetter LRT (eds) Plant tissue culture methods. Saskatoon, Sask., Canada N. R. C. C. pp 60–61

  7. Karp A (1991) On the current understanding of somaclonal variation. In: Miflin BJ (ed) Oxford Surveys of Plant Molecular Cell Biology, vol 7. Oxford University Press, London, pp 1–58

  8. Karp A, Lazzeri PA (1992) Regeneration, stability and transformation of barley. In: Shewry PR (ed) Barley: genetics, biochemistry, molecular biology and biotechnology. C. A. B. International, Wallingford UK, pp 549–571

  9. Karp A, Wu WS, Steele SH, Jones MGK (1987) Chromosome variation in dividing protoplasts and cell suspensions of wheat. Theor Appl Genet 74:140–146

  10. Lazzeri PA, Lörz H (1990) Regenerable suspension and protoplast cultures of barley and stable transformation via DNA uptake into protoplasts. In: Lycett GW, Butterworth DG (eds) Genetic engineering of crop plants. London, pp 231–238

  11. Lazzeri PA, Brettschneider R, Lührs R, Lörz H (1991) Stable transformation of barley via PEG-induced direct DNA uptake into protoplasts. Theor Appl Genet 81:427–444

  12. Lührs R, Lörz H (1988) Initiation of morphogenic cell suspension and protoplast cultures of barley (Hordeum vulgare L.). Planta 175:71–81

  13. Muir WA (1965) Influence of variation in chromosome number and differentiation in plant tissue culture. In: White PR, Grove AP (eds) Proc Congr Plant Tissue Cult. McCutchan, Berkeley, Calif., pp 485–492

  14. Murashige T, Nakano R (1965) Morphogenetic behavior of tobacco tissue cultures and implication of plant senescence. Am J. Bot 52:819–827

  15. Orton TJ (1980) Chromosomal variability in tissue cultures and regenerated plants of Hordeum vulgare L. Theor Appl Genet 56:101–112

  16. Singh RJ (1986) Chromosomal variation in immature embryoderived calluses of barley (Hordeum vulgare L.). Theor Appl Genet 72:710–716

  17. Torrey JG (1967) Morphogenesis in relation to chromosomal constitution in long-term plant tissue cultures. Physiol Plant 20:265–275

  18. Wang X-H, Luo P, Shu J-J (1989) Giemsa N-banding pattern in cabbage and Chinese kale. Euphytica 41:17–21

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Author information

Correspondence to H. Lörz.

Additional information

Communicated by F. Mechelke

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Wang, X., Lazzeri, P.A. & Lörz, H. Chromosomal variation in dividing protoplasts derived from cell suspensions of barley (Hordeum vulgare L.). Theoret. Appl. Genetics 85, 181–185 (1992). https://doi.org/10.1007/BF00222857

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Key words

  • Chromosome variation
  • Embryogenic/regenerable suspension
  • Hordeum vulgare L.
  • Protoplasts