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Effects of sodium butyrate, a new pharmacological agent, on cells in culture

Summary

Sodium butyrate, at millimolar concentrations, when added to cell cultures produces many morphological and biochemical modifications in a reversible manner. Some of them occur in all cell lines. They concern regulatory mechanisms of gene expression and cell growth: an hyperacetylation of histone resulting from an inhibition of histone deacetylase and an arrest of cell proliferation are almost constantly observed. Some other modifications vary from one cell type to another: induction of proteins, including enzymes, hormones, hemoglobin, inhibition of cell differentiation, reversion of transformed characteristics of cells to normal morphological and biochemical pattern, increase in interferon antiviral efficiency and induction of integrated viruses. Most if not all these effects of butyrate could result from histone hyperacetylation, from changes in chromatin structures as measured by accessibility to DNases and from modifications in cytoskeleton assembly. We do not know at the present time whether butyrate acts on a very specific target site in cell or if it acts on several cell components.

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References

  1. 1.

    Prasad, K. N. & Sinha, P. K., 1976. In Vitro 12: 125–132.

  2. 2.

    Riggs, M. G., Whittaker, R. G., Neumann, J. R. & Ingram, V. M., 1977. Nature 268: 462–464.

  3. 3.

    Wright, J. A., 1975. Exp: Cell Res. 78: 456–460.

  4. 4.

    Leder, A. & Leder, P., 1975. Cell 5: 319–322.

  5. 5.

    Ruiz-Carillo, A., Wangh, L. & Allfrey, V. G., 1975. Science 190: 117–128.

  6. 6.

    Davie, J. R. & Candido, E. P. M., 1977. J. Biol. Chem. 252: 5962–596.

  7. 7.

    Gallwitz, D. & Sures, I., 1972. Biochim. Biophys. Acta 263: 315–328.

  8. 8.

    Kamiyama, M., Dastugue, B., Defer, N. & Kruh, J., 1972. Biochim. Biophys. Acta 277: 576–583.

  9. 9.

    Sures, I. & Gallwitz, D., 1980. Biochemistry 19: 943–951.

  10. 10.

    Libby, P. R., 1978. J. Biol. Chem. 253: 233–237.

  11. 11.

    Fukushima, M., Ota, K., Fujimoto, D. & Horiuchi, K., 1980. Biochem. Biophys. Res. Com. 92: 1409–1414.

  12. 12.

    Belikoff, E., Wong, L. J. & Alberts, B. M., 1980. J. Biol. Chem. 255: 11448–11453.

  13. 13.

    Garcea, R. L. & Alberts, B. M., 1980. J. Biol. Chem. 255: 11454–11463.

  14. 14.

    Vidali, G., Boffa, L. C. & Allfrey, V. G., 1972. J. Biol. Chem. 247: 7365–7373.

  15. 15.

    Cousens, L. S., Gallwitz, D. & Alberts, B. M., 1979. J. Biol. Chem. 254: 1716–1723.

  16. 16.

    Jackson, V., Shires, A., Chalkley, R. & Granner, D. K., 1975. J. Biol. Chem. 250: 4856–4863.

  17. 17.

    Sterner, R., Vidali, G., Heinrickson, R. L. & Allfrey, V. G., 1978. J. Biol. Chem. 253: 7601–7604.

  18. 18.

    Sterner, R., Vidali, G. & Allfrey, V. G., 1979. J. Biol. Chem. 254: 11577–11583.

  19. 19.

    Reeves, R. & Candido, E. P. M., 1980. Nucleic Acids Res. 8: 1947–1963.

  20. 20.

    Candido, E. P., Reeves, R. & Davie, J. R., 1978. Cell 14: 105–113.

  21. 21.

    Sealy, L. & Chalkley, R., 1978. Cell 14: 115–121.

  22. 22.

    Boffa, L. C., Vidali, G., Mann, R. S. & Allfrey, V. G., 1978. J. Biol. Chem. 253: 3364–3366.

  23. 23.

    Vidali, G., Boffa, L. C., Mann, R. S. & Allfrey, V. G., 1978. Biochem. Biophys. Res. Com. 82: 223–227.

  24. 24.

    Covault, J. & Chalkley, R., 1980. J. Biol. Chem. 255: 9110–9116.

  25. 25.

    Allfrey, V. G., Faulkner, R. & Mirsky, A. E., 1964. Proc. Natl. Acad. Sci. USA 51: 786–793.

  26. 26.

    Pogo, B. G. T., Allfrey, V. G. & Mirsky, A. E., 1966. Proc. Natl. Acad. Sci. USA 55: 805–812.

  27. 27.

    Pogo, B. G. T., Pogo, A. O., Allfrey, V. G. & Mirsky, A. E., 1968. Proc. Natl. Acad. Sci. U.S.A. 59: 1337–1344.

  28. 28.

    Berlowitz, L. & Pallotta, D., 1972. Exp. Cell Res. 71: 45–48.

  29. 29.

    Gorovsky, M. A., Pleger, G. L., Keevert, J. B. & Johmann, C. A., 1973. J. Cell. Biol. 57: 773–781.

  30. 30.

    Wong, T. K. & Marushige, K., 1976. Biochemistry 15: 2041–2053.

  31. 31.

    Wallace, R. B., Sargent, T. D., Murphy, R. F. & Bonner, J., 1977. Proc. Natl. Acad. Sci. USA 74: 3244–3248.

  32. 32.

    Marushige, K., 1976. Proc. Natl. Acad. Sci. USA 73: 3937–3941.

  33. 33.

    Oberhauser, H., Csordas, A., Puschendorf, B. & Grunicke, H., 1978. Biochem. Biophys. Res. Com. 84: 110–116.

  34. 34.

    Bode, J., Henco, K. & Wingender, E., 1980. Europ. J. Biochem. 110: 143–152.

  35. 35.

    Weintraub, H. & Groudine, M., 1976. Science 193: 848–856.

  36. 36.

    Garel, A. & Axel, R., 1976. Proc. Natl. Acad. Sci. USA 73: 3966–3970.

  37. 37.

    Nelson, D. A., Perry, W. M. & Chalkely, R., 1978. Biochem. Biophys. Res. Com. 82: 356–363.

  38. 38.

    Davie, J. R. & Candido, E. P. M., 1980. FEBS Letters 110: 164–168.

  39. 39.

    Simpson, R. T., 1978. Cell 13: 691–699.

  40. 40.

    Vidali, G., Boffa, L. C., Bradbury, E. M. & Allfrey, V. G., 1978. Proc. Natl. Acad. Sci. USA 75: 2239–2243.

  41. 41.

    Nelson, D. A., Perry, M., Sealy, L. & Chalkley, R., 1978. Biochem. Biophys. Res. Com. 82: 1346–1353.

  42. 42.

    Sealy, L. & Chalkley, R., 1978. Nucleic Acids Res. 5: 1863–1876.

  43. 43.

    Nelson, D., Perry, M. E. & Chalkley, R., 1979. Nucleic Acids Res. 6: 561–574.

  44. 44.

    Shewmaker, C. K., Cohen, B. N. & Wagner, T. E., 1978. Biochem. Biophys. Res. Com. 84: 342–349.

  45. 45.

    Gottesfeld, J. M., Garrard, W. T., Bagi, G., Wilson, R. F. & Bonner, J., 1974. Proc. Natl. Acad. Sci. USA 71: 2193–2197.

  46. 46.

    Davie, J. R. & Candido, P. M., 1978. Proc. Natl. Acad. Sci. USA 75: 3574–3577.

  47. 47.

    Levy-Wilson, B., Gjerset, R. A. & McCarty, B. J., 1977. Biochim. Biophys. Acta 475: 168–175.

  48. 48.

    Shaw, B. R., Corden, J. L., Sahasrabuddhe, C. G. & Van Holde, K. E., 1974. Biochem. Biophys. Res. Com. 61: 1193–1198.

  49. 49.

    Bloom, K. S. & Anderson, J. N., 1978. Cell 15: 141–150.

  50. 50.

    Bellard, M., Gannon, F. & Chambon, P., 1978. Cold Spring Harbor Sympos. Quant. Biol. 42: 779–791.

  51. 51.

    Levy-Wilson, B., Watson, D. C. & Dixon, G. H., 1979. Nucleic Acids Res. 6: 259–274.

  52. 52.

    Kitzis, A., Tichonicky, L., Defer, N. & Kruh, J., 1980. Europ. J. Biochem. 111: 237–244.

  53. 53.

    Mathis, D. J., Oudet, P., Wasylyk, B. & Chambon, P., 1978. Nucleic Acids Res. 5: 3523–3547.

  54. 54.

    Nelson, D., Covault, J. & Chalkley, R., 1980. Nucleic Acids Res. 8: 1745–1763.

  55. 55.

    Dobson, M. E. & Ingram, V. M., 1980. Nucleic Acids Res. 8: 4201–4219.

  56. 56.

    Reeves, R. & Candido, E. P. M., 1979. Biochem. Biophys. Res. Com. 89: 571–579.

  57. 57.

    Reeves, R. & Cserjesi, P., 1979. J. Biol. Chem. 254: 4283–4290.

  58. 58.

    Terada, M., Nudel, U., Fibach, E., Rifkind, R. A. & Marks, P. A., 1978. Cancer Res. 38: 835–840.

  59. 59.

    Sher, W. & Friend, C., 1978. Cancer Res. 38: 841–849.

  60. 60.

    Rastl, E. & Swetly, P., 1978. J. Biol. Chem. 253: 4333–4340.

  61. 61.

    Keppel, F., Allet, B. & Eisen, H., 1977. Proc. Natl. Acad. Sci. USA 74: 653–656.

  62. 62.

    D'Anna, J. A., Tobey, R. A. & Gurley, L. R., 1980. Biochemistry 19: 2656–2671.

  63. 63.

    Leavitt, J. & Moyzis, R., 1978. J. Biol. Chem. 253: 2497–2500.

  64. 64.

    Kitzis, A., Tichonicky, L., Defer, N. & Kruh, J., 1980. Biochem. Biophys. Res. Com. 93: 833–841.

  65. 65.

    Defer, N., Kitzis, A., Levy, F., Tichonicky, L., Sabatier, M. M. & Kruh, J., 1978. Europ. J. Biochem. 88: 583–591.

  66. 66.

    Kitzis, A., Tichonicky, L., Defer, N. & Kruh, J., 1980. Europ. J. Biochem. 111: 237–244.

  67. 67.

    Kitzis, A., Tichonicky, L., Defer, N. & Kruh, J., 1980. Biochem. Biophys. Res. Com. 97: 530–537.

  68. 68.

    Leibovitch, M. P., Leibovitch, S. A., Harel, J. & Kruh, J., 1979. Eur. J. Biochem. 97: 321–326.

  69. 69.

    Leibovitch, M. P., Leibovitch, S. A., Harel, J. & Kruh, J., (submitted).

  70. 70.

    Weingarten, D. & Vellis, J. de, 1980. Biochem. Biophys. Res. Com. 93: 1297–1304.

  71. 71.

    McKnight, G. S., Hager, L. & Palmiter, R. D., 1980. Cell 22: 469–477.

  72. 72.

    Tichonicky, L., Defer, N., Kruh, J., Santana-Calderon, M. A., Giesen, E. M. & Beck, G., Eur. J. Biochem. (in press).

  73. 73.

    Littlefield, B. A., Cidlowski, N. B. & Cidlowski, J. A., 1980. Arch. Biochem. Biophys. 201: 174–184.

  74. 74.

    Samuels, H. H., Stanley, F., Casanova, J. & Shao, T. C., 1980. J. Biol. Chem. 255: 2499–2508.

  75. 75.

    Wright, J. A., 1973. Exp. Cell Res. 78: 456–460.

  76. 76.

    Ginsburg, E., Salomon, D., Sreevalsan, T. & Freese, E., 1973. Proc. Natl. Acad. Sci. USA 70: 2457–2461.

  77. 77.

    Leibovitch, M. P. & Kruh, J., 1979. Biochem. Biophys. Res. Com. 87: 896–903.

  78. 78.

    Hagopian, H. K., Riggs, M. G., Swartz, L. A. & Ingram, V. M., 1977. Cell 12: 855–860.

  79. 79.

    Wijk, R. van, Tichonicky, L. & Kruh, J. Submitted.

  80. 80.

    D'Anna, J. A., Gurley, L. R., Becker, R. R., Barham, S. S., Tobey, R. A. & Walters, R. A., 1980. Biochemistry 19: 4331–4341.

  81. 81.

    Fallon, R. J. & Cox, R. P., 1979. J. Cell Physiol. 100: 251–260.

  82. 82.

    Nadler, K. D., 1976. Exp. Cell Res. 101: 283–292.

  83. 83.

    D'Anna, J. A., Tobey, R. A., Barham, S. S. & Gurley, L. R., 1977. Biochem. Biophys. Res. Com. 77: 187–194.

  84. 84.

    Moore, M., Jackson, V., Sealy, L. & Chalkley, R., 1979. Biochim. Biophys. Acta 561: 248–260.

  85. 85.

    Chahal, S. S., Matthews, H. R. & Bradbury, E. M., 1980. Nature 287: 76–79.

  86. 86.

    Altenburg, B. C., Via, D. P. & Steiner, S. H., 1976. Exp. Cell Res. 102: 223–231.

  87. 87.

    Altenburg, B. C. & Steiner, S. H., 1979. Exp. Cell Res. 118: 31–37.

  88. 88.

    Borenfreund, E., Schmid, E., Bendich, A. & Franke, W. W., 1980. Exp. Cell Res. 127: 215–235.

  89. 89.

    Borenfreund, E., Higgins, P. J., Steinglass, M. & Bendich, A., 1979. Cancer Res. 39: 800–807.

  90. 90.

    Hayman, E. G., Engvall, E. & Ruoslahti, E., 1980. Exp. Cell. Res. 127: 478–481.

  91. 91.

    Leavitt, J., Barrett, J. C., Crawford, B. D. & TS'O, P. O. P., 1978. Nature 271: 262–265.

  92. 92.

    Bourgeade, M. F., Cerutti, I. & Chany, C., 1979. Cancer Res. 39: 4720–4723.

  93. 93.

    Bourgeade, M. F., Rousset, S., Paulin, D. & Chany, C. J., 1981. Interferon Res., 1: 323–332.

  94. 94.

    Bourgeade, M. F. & Chany, C., 1979. Int. J. Cancer 24: 314–318.

  95. 95.

    Bourgeade, M. F., Chany, C. & Merigan, T. C., 1970. J. Gen. Virol. 46: 449–454.

  96. 96.

    Adolf, G. R. & Swetly, P., 1979. Virology 99: 158–166.

  97. 97.

    Macher, B. A., Lockney, M., Moskal, J. R., Fung, Y. K. & Sweeley, C. C., 1978. Exp. Cell Res. 117: 95–102.

  98. 98.

    Guillouzo, A., Tichonicky, L., Boisnard-Rissel, M. & Kruh, J., 1980. Cell Biol. Intern. Rep. 4: 961–968.

  99. 99.

    Mori, Y., Akedo, H., Tanaka, K., Tanigaki, Y. & Okada, M., 1979. Exp. Cell Res. 118: 15–22.

  100. 100.

    Tralka, T. S., Rabson, A. S., Thorgeirsson, U. P. & Tseng, J. S., 1979. Proc. Soc. Exp. Biol. Med. 161: 543–545.

  101. 101.

    Waymire, J. C., Weiner, N. & Prasad, K. N., 1972. Proc. Natl. Acad. Sci. USA 69: 2241–2245.

  102. 102.

    Marks, P. A. & Rifkind, R. A., 1978. Ann. Rev. Biochem. 47: 419–448.

  103. 103.

    Leder, A. & Leder, P., 1975. Cell 5: 319–322.

  104. 104.

    Cioe, L., McNab, A., Hubbell, H. R., Hed, P., Curtis, P. & Rovera, G., 1981. Cancer Res. 41: 237–243.

  105. 105.

    Chou, J. Y., Robinson, J. C. & Wang, S., 1977. Nature 268: 543–44.

  106. 106.

    Ghosh, N., Rukenstein, A. & Cox, R. P., 1977. Biochem. J. 166: 265–274.

  107. 107.

    Tralka, T. S., Rosen, S. W., Weintraub, B. D., Lieblich, J. M., Engel, L. W., Wetzel, B. K., Kingsbury, E. W. & Rabson, A. S., 1979. J. Natl. Cancer Inst. 62: 45–61.

  108. 108.

    Ghosh, N. K. & Cox, R. P., 1977. Nature 267: 435–437.

  109. 109.

    Griffin, M. J., Price, G. H., Bazell, K. L., Cox, R. P. & Ghosh, N. K., 1974. Arch. Biochem. Biophys. 164: 619–623.

  110. 110.

    Tallman, J. F., Smith, C. C. & Henneberry, R. C., 1977. Proc. Natl. Acad. Sci. USA 74: 873–877.

  111. 111.

    Lin, C. & Lin, M. C., 1979. Exp. Cell Res. 122: 399–402.

  112. 112.

    Blau, H. M. & Epstein, C. J., 1979. Cell 17: 95–108.

  113. 113.

    Fiszman, M. Y., Montarras, D., Wright, W. & Gros, F., 1980. Exp. Cell Res. 126: 31–37.

  114. 114.

    Borenfreund, E., Higgins, P. J. & Bendich, A., 1980. Annals N.Y. Acad. Sci. 349: 357–371.

  115. 115.

    Mori, Y., Akedo, H., Tanigaki, Y., Tanaka, K. M., Okada, M. & Nakamura, N., 1980. Exp. Cell Res. 127: 465–470.

  116. 116.

    Fishman, P. H., Simmons, J. L., Brady, R. O. & Freese, E., 1974. Biochem. Biophys. Res. Com. 59: 292–299.

  117. 117.

    Fishman, P. H. & Brady, R. O., 1976. Science 194: 906–915.

  118. 118.

    Fishman, P. H., Bradley, R. M., Henneberry, R. C., 1976. Arch. Biochem. Biophys. 172: 618–626.

  119. 119.

    Henneberry, R. C. & Fishman, P. H., 1976. Exp. Cell Res. 103: 55–62.

  120. 120.

    Via, D. P., Sramek, S., Larriba, G. & Steiner, S., 1980. J. Cell Biol. 84: 225–234.

  121. 121.

    Long, C. W., Suk, W. A., Snead, R. M. & Christensen, W. L., 1980. Cancer Res. 40: 3886–3890.

  122. 122.

    Luka, J., Kallin, B. & Klein, G., 1979. Virology 94: 228–231.

  123. 123.

    Kallin, B., Luka, J. & Klein, G., 1979. J. Virol. 32: 710–716.

  124. 124.

    Saemundsen, A. K., Kallin, B. & Klein, G., 1980. Virology 107: 557–561.

  125. 125.

    Daniell, E., 1980. Virology 107: 514–519.

  126. 126.

    Chester, A. & Yaniv, M., 1979. Proc. Natl. Acad. Sci. USA 76:46–50.

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Kruh, J. Effects of sodium butyrate, a new pharmacological agent, on cells in culture. Mol Cell Biochem 42, 65–82 (1981). https://doi.org/10.1007/BF00222695

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Keywords

  • Cell Growth
  • Interferon
  • Cell Differentiation
  • Regulatory Mechanism
  • Butyrate