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The peribrachial region of cat

II. Distribution of acetylthiocholinesterase and monoaminoxidase, with special reference to the locus coeruleus


The locus coeruleus and the tegmental region within which it is encased are very rich in acetylthiocholinesterase (AThChE). The same applies to the pontomesencephalic griseum centrale and the substantia nigra. As a result, the mesencephalon displays a central tegmental core, poor in AThChE, surrounded by a collar-shaped region made out of territories which stain intensely with the technique of Koelle and Friedenwald. By contrast, in material stained with Glenner's technique, only the locus coeruleus and a relatively circumscribed rostrolateral tegmental region appear darkly stained, while the adjacent tegmentum entobrachiale remains pale. The possibility that the entobrachial tegmentum and the locus coeruleus should constitute a cholinoceptive-adrenergic system is discussed.

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  1. Anden, N. E., Dahlström, A., Fuxe, K., Larsson, K.: Mapping out of catecholamine and 5-hydroxy-tryptamine neurons innervating the telencephalon and diencephalon. Life Sci. 4, 1275–79 (1965)

  2. Anden, N. E. Dahlström, A., Fuxe, K., Larsson, K., Olson, L., Ungerstedt, U.: Ascending monoamine neurons to the telencephalon and diencephalon. Acta physiol. scand. 67, 313–326 (1966)

  3. Anden, N. E., Fuxe, K., Ungerstedt, U.: Monoamine pathways to the cerebellar and cerebral cortex. Experientia (Basel) 23, 838–839 (1967)

  4. Berman, A. L.: The brain stem of the cat. A cytoarchitectonic atlas with stereotaxic coordinates. University of Wisconsin Press. Wisconsin: Madison 1968

  5. Björklund, A., Falck, B., Stenevi, U.: Classification of monoamine neurons in the rat mesencephalon: distribution of a new monoamine neurone system. Brain Res. 32, 269–285 (1971)

  6. Blackstad, T., Fuxe, K., Hökfelt, T.: Noradrenaline nerve terminals in the hippocampal region of the rat and the guinea pig. Z. Zellforsch. 78, 463–473 (1967)

  7. Bloom, F. E., Hoffer, B. B., Siggins, G. R.: Studies on norepinephrine containing afferents to Purkinje cells of rat cerebellum. I. Localisation of the fibres and their synapses. Brain Res. 25, 501–521 (1971)

  8. Collins, G.G.S., Sandler, M., Williams, E. D., Youdim, M. B. H.: Multiple forms of human brain mitochondrial monoamineoxidase. Nature (Lond.) 225, 817–820 (1970)

  9. Dahlström, A., Fuxe, K.: Evidence for the existence of monoamine containing neurons in the central nervous system. I. Demonstration of monoamines in the cell bodies of brain stem neurons. Acta physiol. scand. 62, (suppl. 232), 1–80 (1970)

  10. Eccles, J. C., Fatt, P., Koketsu, K.: Cholinergic and inhibitory synapses in a pathway from motor axon collaterals to motoneurons. J. Physiol. (Lond.) 126, 425–562 (1954)

  11. Eränkö, O.: Demonstration of catecholamines and cholinesterases in the same section. Pharmacol. Rev. 18, 353–358 (1966)

  12. Falck, B., Hillarp, N. A., Thieme, G., Torp, A.: Fluorescence of catecholamines and related compounds condensed with formaldehyde. J. Histochem. Cytochem. 10, 348–354 (1962)

  13. Feldberg, W., Mann, T.: Properties and distribution of the enzyme system which synthesizes acetylcholine in nervous tissue. J. Physiol. (Lond.) 104, 411–425 (1946)

  14. Feldberg, W., Vogt, T.: Acetyloholine synthesis in different regions of the central nervous system. J. Physiol. (Lond.) 107, 372–381 (1948)

  15. Fuxe, K.: Evidence for the existence of monoamine neurons in the central nervous system. Acta physiol. scand. 65 (Suppl. 247) 37–84 (1965 a)

  16. Fuxe, K.: Evidence for the existence of monoamine neurons in the central nervous system. III. The monoamine nerve terminal. Z. Zellforsch. 65, 573–596 (1965 b)

  17. Fuxe, K., Hamberger, B., Hökfelt, T.: Distribution of noradrenaline nerve terminals in cortical areas of the rat. Brain Res. 28, 125–131 (1968)

  18. Fuxe, K., Hökfelt, T., Wilsson, O.: A fluorescence and electron microscopic study on certain brain regions rich in monoamine terminals. Amer. J. Anat. 117, 33–46 (1965)

  19. George, R., Hasslett, W. L., Jenden, D. J.: A cholinergic mechanism in the brain stem reticular formation: induction of paradoxical sleep. Int. J. Neuropharmacol. 3, 541–552 (1964)

  20. Glenner, G. G., Burtner, H. J., Brown, G. H.: The histochemical demonstration of monoamineoxidase activity by tetrazolium salts. J. Histochem. Cytochem. 5, 591–600 (1957)

  21. Gomori, G.: Microscopic histochemistry. Chicago: Chicago University Press 1952

  22. Hall, E., Genesser-Jensen, F. A.: Distribution of acetylcholinesterase and monoamine oxydase in the amygdala of the guinea pig. Z. Zellforsch. 120, 204–221 (1971)

  23. Jouvet, M.: Neurophysiology of the states of sleep, In: The neurosciences (G. C. Quarton, T. Melnechuck and F. O. Schmitt, eds.). New York: Rockefeller Univ. Press 1967 a

  24. Jouvet, M.: Neurophysiology of the states of sleep. Physiol. Rev. 47, 117–177 (1967 b)

  25. King, E. J., Sperri, W. M.: Biochemistry handbook. London: Spon. 1961

  26. Koelle, G. B., Friedenwald, J. S.: A method for localizing cholinesterase activity. Proc. Soc. exp. Biol. (N.Y.) 70, 617 622 (1949)

  27. Lewis, P. R., Shute, G.C.D., Silver, A.: Confirmation from choline acetylase analysis of a massive cholinergic innervation to the rat hippocampus. J. Physiol. (Lond.) 191, 215–224 (1967)

  28. MacIntosh, F. C.: The distribution of acetylcholine in the peripheral and the central nervous system. J. Physiol. (Lond.) 99, 436–442 (1941)

  29. Maeda, T., Abe, T., Shimizu, N.: Histochemical demonstration of aromatic monoamine in locus coeruleus of the mammalian brain. Nature (Lond.) 188, 326–327 (1960)

  30. Maeda, T., Gerebtzoff, M. A.: Recherches sur les développement du locus coeruleus. II. Etude histoenzymologique. Acta neurol. belg. 69, 11–19 (1969)

  31. Manocha, S. L., Shanta, T. R., Bourne, G. H.: Histochemical mapping of the distribution of monoamine oxidase in the diencephalon and basal telencephalic centers of the brain of squirrel monkey. Brain Res. 6, 570–586 (1967)

  32. Olson, L., Fuxe, K.: On the projections from the locus coeruleus noradrenaline neurons: the cerebellar innervation. Brain Res. 28, 165–171 (1971)

  33. Pin, C., Jones, B., Jouvet, M.: Topographie des neurones monoaminergiques du tronc cérébral du chat: étude sur histofluorescence. C. R. Soc. Biol. (Paris) 163, 2136–2141

  34. Poirier, L. J., Sourkes, T. L.: Influence of the substantia nigra on the catecholamine content of the striatum. Brain 88, 181–192 (1965)

  35. Ramon-Moliner, E.: Acetylthiocholinesterase distribution in the brain stem of the cat. Ergebn. Anat. Entwickl.-Gesch. 46 (4), 1–53 (1972)

  36. Shimizu, N., Matsunami, T., Onishi, S.: Histochemical demonstration of ascorbic acid in the locus coeruleus of the mammalian brain. Nature (Lond.) 186, 479–480 (1960)

  37. Shimizu, U., Morikawa, N.: Histochemical study of monoamine oxidase in the developing rat brain. Nature (Lond.) 184, 650–651 (1959)

  38. Silver, A.: Cholinesterases of the central nervous system with special reference to the cereellum. Int. Rev. Neurobiol. 10, 57–109 (1967)

  39. Snider, R. S., Niemer, W. T.: A stereotaxic atlas of the cat brain. Chicago: Chicago University Press 1961

  40. Spector, W. S.: Handbook of biological data. Philadelphia: Saunders. (1956)

  41. Taber, E.: The cytoarchitecture of the brain stem in the cat. J. comp. Neurol. 116, 27–69 (1961)

  42. Ungerstedt, U.: Stereotopic mapping of monoamine pathways in rat brain. Acta physiol. scand. 1, 71 (1971)

  43. Verhaart, W. J. C.: A stereotaxic atlas of the brain stem of the cat. Netherlands: Van Gorcum 1964

  44. Whittaker, M.: The serum cholinesterase variants. Differentiation by means of formaldehyde. Clin. chim. Acta 26, 141–145 (1969)

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Supported by grant MA 4183 from the Medical Research of Canada.

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Ramon-Moliner, E., Dansereau, J.A. The peribrachial region of cat. Cell Tissue Res. 149, 191–204 (1974).

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Key words

  • Locus coeruleus
  • Tegmentum
  • Acetylcholinesterase
  • Monoaminoxidase
  • Paradoxical sleep