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The use of mitochondrial DNA polymorphism in the classification of individual onion plants by cytoplasmic genotypes


Individual plants of a Japanese onion variety ‘Sapporo-ki’, which is characterized by the occasional occurrence of male-sterile plants, have been investigated for mitochondrial (mt) DNA polymorphism. Male-fertile and the Jones' cytoplasmic male-sterile (CMS) onions were also included for comparison. Southern blot hybridization with rrn26, cox-I, cox-II, cob, atpA and atp9 genes as probes revealed the two classes of mtDNA variation within a population of ‘Sapporo-ki’: Out of the 41 plants examined 19 contained mtDNA typical of malefertile plants, and 22 individuals contained mtDNA typical of the Jones' CMS genotype. Our results thus indcate that the use of the mitochondrial gene probes may greatly facilitate the classification of individual plants by cytoplasmic genotypes.

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  1. Boer PH, McIntosh JE, Gray MW, Bonen L (1985) The wheat mitochondrial gene for apocytochrome b: absence of a prokaryotic ribosome binding site. Nucleic Acids Res 13:2281–2292

  2. De Courcel AGL, Vedel F, Boussac JM (1989) DNA polyphism in Allium cepa cytoplasms and its implications concerning the origin of onions. Theor Appl Genet 77:793–798

  3. Dowker BD (1990) Onion breeding. In: Rabinowitch HD, Brewster JL (eds) Onions and allied crops. CRC Press, Florida, pp 215–232

  4. Holford F, Newbury HJ, Croft JH (1988) Differences in the mitochondrial DNA of male-fertile, cms S and cms T onions. In: Riggs TJ, Institute of Horticultural Research, Wellesbourne, United Kingdom Proc 4th Eucarpia Allium Symp. pp 205–211

  5. Holford F, Croft JH, Newbury HJ (1991) Differences between, and possible origins of, the cytoplasms found in fertile and male-sterile onions (Allium cepa L.). Theor Appl Genet 82:737–744

  6. Huh TY, Gray MW (1982) Conservation of ribosomal RNA gene arrangement in the mitochondrial DNA of angiosperms. Plant Mol Biol 1:245–249

  7. Jones HA, Emsweller SL (1936) A malesterile onion. Proc Am Soc Hortic Sci 34:582–585

  8. Morikami A, Nakamura K (1987 a) Structure and expression of pea mitochondrial F-1 α-subunit gene and its pseudogene involved in homologous recombination. J Biochem 101:967–976

  9. Morikami A, Nakamura K (1987 b) The pea mitochondrial ATPase subunit 9 gene is located upstream of the ATPase α-subunit gene. Nucleic Acids Res 15:4692

  10. Newton KJ (1988) Plant mitochondrial genomes: organization, expression and variation. Annu Rev Plant Physiol Plant Mol Biol 39:503–532

  11. Senda M, Harada T, Mikami T, Sugiura M, Kinoshita T (1991) Genomic organization and sequence analysis of the cytochrome oxidase subunit II gene from normal and malesterile mitochondria in sugarbeet. Curr Genet 19:175–181

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Communicated by D.R. Pring

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Satoh, Y., Nagai, M., Mikami, T. et al. The use of mitochondrial DNA polymorphism in the classification of individual onion plants by cytoplasmic genotypes. Theoret. Appl. Genetics 86, 345–348 (1993).

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Key words

  • Onion
  • Allium cepa
  • Cytoplasmic male sterility
  • Mitochondrial DNA