Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Acid phosphatase localization in neurons of Bulla gouldiana (Gastropoda: Opisthobranchia)

  • 15 Accesses

  • 4 Citations

Summary

The organization of the ganglia and the ultrastructure of the neurons of Bulla gouldiana are similar to those described for other molluscs. Acid phosphatase positive reactions were found in the large pigmented granules, small dense bodies, multivesicular bodies, and Golgi lamellae and associated vesicles. The small dense bodies and multivesicular bodies may be stages in the formation of the larger pigmented granules which are interpreted as lysosomes. Comparison is made between the pigmented granules in Bulla and the lipofuscin bodies of vertebrate neurons. The possible involvement of these pigmented granules in the hyperpolarization of Bulla and Aplysia neurons to light is discussed.

This is a preview of subscription content, log in to check access.

References

  1. Adzhimolaev, T. A., Murav'ëv, R. A., Rogovin, V. V.: Electron cytochemistry of acid phosphatase in giant nervous cells of the nudibranch Tritonia diomedia. Z. H. Evol. Biokhim. Immunobiol. 8, 152–156 (1972)

  2. Amoroso, E. C., Baxter, M. P., Chiquoine, A. D., Nisbet, R. H.: The fine structure of neurons and other elements in the nervous system of the giant African land snail Archachatina marginata. Proc. roy. Soc. B 160, 167–180 (1964)

  3. Arvanitaki, A., Chalazonitis, N.: Ultrastructure des formations intermédiaires probables au cours de la maturation des ≪grains≫ pigmentés du neurone (Aplysia depilans). C. R. Soc. Biol. (Paris) 160, 1017–1020 (1966)

  4. Barka, T., Anderson, P. J.: Histochemical methods for acid phosphatase using hexazonium paraosanilin as coupler. J. Histochem. Cytochem. 10, 741–753 (1962)

  5. Bergh, L. S. R.: Malacologische Untersuchungen, Bd. 5. In: C. Semper, Reisen im Archipel der Philippinen. 5. Teil, 4. Abteilung, 3. Abschnitt, Lieferung 1 u. 2, 209–312 (1901)

  6. Bowen, I.D.: A high resolution technique for the fine structural localization of acid hydrolases. J. Microscopy 94, 25–38 (1971)

  7. Brown, A. M., Brown, H. M.: Light response of a giant Aplysia neuron. J. gen. Physiol. 62, 239–254 (1973)

  8. Brown, H. M., Brown, A.M.: Ionic basis of the photoresponse of Aplysia giant neuron: K+ permeability increase. Science 178, 755–756 (1972)

  9. Bullock, T. H.: On the anatomy of the giant neurons of the visceral ganglion of Aplysia. In: Nervous inhibition, ed. E. Florey, 1st ed., p. 233–240. New York: Pergamon 1961

  10. Bullock, T. H., Horridge, G. A.: Structure and function in the nervous systems of invertebrates, vol. II, 1st ed. San Francisco: Freeman 1965

  11. Chalazonitis, N.: Chemopotentials in giant nerve cells (Aplysia fasciata). In: Nervous inhibition, E. Florey, ed., 1st ed., p. 179–193. New York: Pergamon 1961

  12. Chalazonitis, N.: Light energy conversion in neuronal membranes. Photochem. Photobiol. 3, 539–559 (1964)

  13. Chalazonitis, N., Chagneux-Costa, H., Chagneux, R.: Ultrastructure des ≪grains≫ pigmentés du cytoplasme des neurones d' Aplysia depilans. C.R. Soc. Biol. (Paris) 160, 1014–1017 (1966)

  14. Chou, J. T. Y.: The cytoplasmic inclusions of the neurons of Helix aspersa and Limnaea stagnalis. Quart. J. micr. Sci. 98, 47–58 (1957a)

  15. Chou, J. T. Y.: The chemical composition of lipid globules in the neurons of Helix aspersa. Quart. J. micr. Sci. 98, 59–64 (1957b)

  16. Chou, J. T. Y., Meek, G. A.: The ultra-fine structure of lipid globules in the neurones of Helix aspersa. Quart. J. micr. Sci. 99, 279–287 (1958)

  17. Coggeshall, R. E.: A light and electron microscope study of the abdominal ganglion of Aplysia californica. J. Neurophysiol. 30, 1263–1287 (1967)

  18. Duve, C. de, Wattiaux, R.: Functions of lysosomes. Ann. Rev. Physiol. 28, 435–492 (1966)

  19. Frazier, W. T., Kandel, E. R., Kupfermann, I., Waziri, R., Coggeshall, R. E.: Morphological and functional properties of identified neurons in the abdominal ganglion of Aplysia californica. J. Neurophysiol. 30, 1288–1351 (1967)

  20. Ghiselin, M. T.: Reproductive function and the phylogeny of opisthobranch gastropods. Malacologia 3, 327–378 (1965)

  21. Gomori, G.: Microscopic histochemistry; principles and practice, 1st ed. Chicago: Chicago Univ. Press 1952

  22. Henkart, M.: Structural changes associated with illumination in the Aplysia giant neuron. Soc. for Neuroscience, Program and Abstracts, p. 358 (1973)

  23. Johnson, M. E., Snook, H. J.: Seashore animals of the Pacific coast, 1st ed. New York: Macmillan Company 1927

  24. Karnovsky, M.: A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol. 27, 137A (1965)

  25. Lane, N. J.: Thiamine pyrophosphatase, acid phosphatase, and alkaline phosphatase in the neurons of Helix aspersa. Quart J. micr. Sci. 104, 401–412 (1963)

  26. Lane, N. J.: The fine-structural localization of phosphatases in neurosecretory cells within the ganglia of certain gastropod snails. Amer. Zool. 6, 139–157 (1966)

  27. Marcus, E.: Opisthobranch mollusks from California. Veliger 3 (Suppl. 1), 1–5 (1961)

  28. Meek, G. A., Lane, N. J.: The ultrastructural localization of phosphatases in the neurons of the snail, Helix aspersa. J. roy. micr. Soc. 82, 193–204 (1964)

  29. Pearse, A. G. E.: Histochemistry, theoretical and applied, 3rd ed., vol. II. Edinburgh and London: Churchill Livingstone 1972

  30. Rosenbluth, J.: The visceral ganglion of Aplysia californica. Z. Zellforsch. 60, 213–236 (1963)

  31. Simpson, L., Bern, H. S., Nishioka, R. S.: Inclusions in the neurons of Aplysia californica (Cooper, 1863) (Gastropoda Opisthobranchiata). J. comp. Neurol. 121, 237–258 (1963)

Download references

Author information

Correspondence to Laura J. Robles.

Additional information

Supported by grant EY 00888 from the USPHS and a Faculty Research Grant from the University of California.

The authors wish to thank Dr. I. Charvat for her help with the enzyme localization, Dr. J. Cronshaw for use of his EM facility, and Dr. K. Esau, Prof. B. B. Boycott, and Mr. K. Linberg for their critical reading of the manuscript.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Robles, L.J., Fisher, S.K. Acid phosphatase localization in neurons of Bulla gouldiana (Gastropoda: Opisthobranchia). Cell Tissue Res. 157, 217–225 (1975). https://doi.org/10.1007/BF00222067

Download citation

Key words

  • Ganglia
  • Neurons
  • Pigmented granules
  • Acid phosphatase
  • Lysosomes