Functional myeloperoxidase contained in granules of polymorphonuclear neutrophil leukocytes or in fixed whole cells can be endocytosed by mouse peritoneal macrophages. Acquired myeloperoxidase was distributed in what we considered to be the secondary lysosomal system and, following a phagocytic stimulation, was delivered to newly formed phagosomes containing the targets.
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Babior BM (1978) Oxygen-dependent microbial killing by phagocytes. N Engl J Med 298:659–662
Biggar WD, Buron S, Holmes B (1976) Bactericidal mechanisms in rabbit alveolar macrophages: Evidence against peroxidase and hydrogen peroxide bactericidal mechanisms. Infect Immun 14:6–10
Daems WTh, Poelmann RE, Brederoo P (1973) Peroxidatic activity in resident peritoneal macrophages and exudate monocytes of the guinea pig after ingestion of latex particles. J Histochem Cytochem 21:93–95
Dvorak AM, Klebanoff SJ, Henderson WR, Monahan RA, Pyne K, Galli SJ (1985) Vesicular uptake of eosinophil peroxidase by guinea pig basophils and by cloned mouse mast cells and granule-containing lymphoid cells. Am J Pathol 118:425–438
Furth R van, Hirsch JG, Fedorko ME (1970) Morphology and peroxidase cytochemistry of mouse promonocytes, monocytes, and macrophages. J Exp Med 132:794–812
Goren MB (1977) Phagocyte lysosomes: interactions with infectious agents, phagosomes, and experimental perturbations in function. Annu Rev Microbiol 31:507–533
Heifets L, Imai K, Goren MB (1980) Expression of peroxidase-dependent iodination by macrophages ingesting neutrophil debris. J Reticuloendothel Soc 28:391–404
Hibbs JB Jr, Chapman HA Jr, Weinberg JB (1978) The macrophage as an antineoplastic surveillance cell: biological perspectives. J Reticuloendothel Soc 24:549–570
Kielian MC, Cohn ZA (1980) Phagosome-lysosome fusion. Characterization of intracellular membrane fusion in mouse macrophages. J Cell Biol 85:754–765
Klebanoff SJ (1975) Antimicrobial mechanisms in neutrophilic polymorphonuclear leukocytes. Semin Hematol 12:117–142
Matheson NR, Wong PS, Travis J (1981) Isolation and properties of human neutrophil myeloperoxidase. Biochemistry 20:325–330
Nathan CF (1983) Mechanisms of macrophage antimicrobial activity. Trans R Soc Trop Med Hyg 77:620–630
Nathan C, Brukner L, Kaplan G, Unkeless J, Cohn A (1980) Role of activated macrophages in antibody dependent lysis of tumor cells. J Exp Med 152:183–197
Nichols BA (1981) Use of ultrastructural histochemistry. In: Adams DO, Edelson PJ, Koren HS (eds) Methods for studying mononuclear phagocytes. Academic Press, New York, pp 413–432
Nichols BA (1982) Uptake and digestion of horseradish peroxidase in rabbit alveolar macrophages — Formation of a pathway connecting lysosomes to the cell surface. Lab Invest 47:235–246
Ogawa T, Koerten HK, Daems WTh (1978) Peroxidatic activity in monocytes and tissue macrophages of mice. Cell Tiss Res 188:361–373
Ramsey PG, Martin T, Chi E, Klebanoff SJ (1982) Arming of mononuclear phagocytes by eosinophil peroxidase bound to Staphylococcus aureus. J Immunol 128:415–420
Register KB, Davis CH, Wyrick PB, Shafer WM (1987) Nonoxidative antimicrobial effects of human polymorphonuclear leukocyte granule proteins on Chlamydia spp. in vitro. Infect Immun 55:2420–2427
Shellito J, Sniezek M, Warnock M (1987) Acquisition of peroxidase activity by rat alveolar macrophages during pulmonary inflammation. Am J Pathol 129:567–577
Simmons SR, Karnovsky ML (1973) Iodinating ability of various leukocytes and their bactericidal activity. J Exp Med 138:44–63
Steinman RM, Brodie SE, Cohn ZA (1976) Membrane flow during pinocytosis — A stereologic analysis. J Cell Biol 68:665–687
Spitznagel JK (1977) Bactericidal mechanisms in the granulocyte. In: Greenwalt TJ, Jamieson GA (eds) The granulocyte: function and clinical utilization. Alan R. Liss, New York, pp 103–131
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Leung, K.-., Goren, M.B. Uptake and utilization of human polymorphonuclear leukocyte granule myeloperoxidase by mouse peritoneal macrophages. Cell Tissue Res. 257, 653–656 (1989). https://doi.org/10.1007/BF00221477
- Polymorphonuclear leukocytes
- Mouse (C57BL/6)