Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Localization of vitamin A in the small intestine of mouse

An electron microscope radioautographic study


Localization of vitamin A in the small intestine of mice was studied with electron microscope radioautography after administration of tritiated vitamin A. The label was concentrated over lipid droplets in cells distributed in the lamina propria and the submucous layer. The cells were similar both to fibroblasts and to fat-storing cells in their morphological features. The name “Vitamin A-Storing Cell” is proposed for these labeled cells, including the fat-storing cell in the liver.

This is a preview of subscription content, log in to check access.


  1. Bahu, R., Minick, O.T., Inouye, T., Kent, G., Popper, H.: The dual function of hepatic lipocytes. J. Cell Biol. 67, 15a (1975)

  2. Eakin, R.M., Brandenburger, J.L.: Localization of vitamin A in the eye of a pulmonate snail. Proc. nat. Acad. Sci. (Wash.) 60, 140–145 (1968)

  3. Hirosawa, K., Yamada, E.: The localization of the vitamin A in the mouse liver as revealed by electron microscope radioautography. J. Electron Micros. 22, 337–346 (1973)

  4. Hirosawa, K., Yamada, E.: Localization of 3H-vitamin A in the mouse lung as revealed by electron microscope radioautography. Acta anal. Nippon. 49, 60 (1974)

  5. Hirosawa, K., Yamada, E.: Localization of vitamin A in the mouse retina as revealed by radioautography. Jap. J. Ophthal. (in press) (1976)

  6. Ito, T., Shibasaki, S.: Electron microscopic study on the hepatic sinusoidal wall and the fat-storing cells in the normal human liver. Arch. Histol. Jap. 29, 137–192 (1968)

  7. Kobayashi, K., Takahashi, Y.: Effect of the administration of large doses of vitamin A on the fine structure of rat liver with special reference to changes in fat-storing cell. Arch. Histol. Jap. 33, 421–443 (1971)

  8. Moore, T.: Vitamin A. Amsterdam-London-New York: Elsevier Pub. Co. 1957

  9. Morgan, T.E., Huber, G.L.: Loss of lipid during fixation for electron microscopy. J. Cell Biol. 32, 757–760 (1967)

  10. Muto, Y., Smith, J.E., Milch, P.O., Goodman, DeW.S.: Regulation of retinol-binding protein metabolism by vitamin A status in the rat. J. biol. Chem. 247, 2542–2550 (1972)

  11. Plopper, C.G., Wallace, D.L., Pietzsch, J.D.: Extraction of C14-vitamin A from rat liver and kidney during processing for transmission electron microscopy. Stain Technol. 50, 339–345 (1975)

  12. Poole, A.R., Dingle, J.T., Mallia, A.K., Goodman, DeW.S.: The localization of retinol-binding protein in rat liver by immunofluorescence microscopy. J. Cell Sci. 19, 379–394 (1975)

  13. Popper, H.: Distribution of vitamin A in tissue as visualized by fluorescence microscopy. Physiol. Rev. 24, 205–224 (1944)

  14. Revel, J.-P., Hay, E.: An autoradiographic and electron microscopic study of collagen synthesis in differentiating cartilage. Z. Zellforsch. 61, 110–144 (1963)

  15. Ross, R.: The connective tissue forming cell. In: Treatise on collagen (G.N. Ramachandran, ed.), Vol. 2, pp. 1–82. New York: Acad. Press 1968

  16. Stein, O., Stein, Y.: Lipid synthesis, intracellular transport, storage and secretion I. Electron microscopic radioautographic study of liver after injection of tritiated palmitate or glycerol in fasted and ethanol-treated rats. J. Cell Biol. 33, 319–339 (1967)

  17. Wake, K.: “Sternzellen” in the liver: perisinusoidal cells with special reference to storage of vitamin A. Amer. J. Anat. 132, 429–462 (1971)

  18. Wake, K.: Development of vitamin A-rich lipid droplets in multivesicular bodies of rat liver stellate cells. J. Cell Biol. 63, 683–691 (1975)

  19. Yamagishi, M.: Electron microscopic studies on the fine structure of the sinusoidal wall and fat-storing cells of rabbit liver. Arch. Histol. Jap. 18, 223–261 (1959)

Download references

Author information

Correspondence to Assoc. Prof. Dr. Kazushige Hirosawa.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hirosawa, K., Yamada, E. Localization of vitamin A in the small intestine of mouse. Cell Tissue Res. 177, 57–65 (1977).

Download citation

Key words

  • Vitamin A
  • Small intestine
  • Fat-storing cell
  • Electron microscope radioautography