Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Molecular tagging of the dwarf BREIZH (Bzh) gene in Brassica napus

  • 221 Accesses

  • 45 Citations

Abstract

We mapped the dwarf Bzh gene in B. napus with RAPD and RFLP markers. Research of the linked markers proceeded in two ways: a random approach through the construction of a detailed genetic map and targeting of the dwarf gene using both near-isogenic lines (NILs) and the bulked segregant analysis (BSA) method. The BSA approach was the most efficient in finding DNA markers linked to Bzh, whereas the efficiency of the NILs approach was limited by a too great similarity of the genetic background between the dwarf donor parent and the recurrent lines. Eight RAPD markers were identified as linked to Bzh, the closest being at 0.8±0.7 cM. The random genetic mapping approach added markers and extended the linkage group containing Bzh. This work represents the first step towards a better understanding of the dwarf mutation, the development of marker-assisted selection, and the cloning of the underlying gene responsible for dwarfing.

This is a preview of subscription content, log in to check access.

References

  1. Bailey NTJ (1949) The estimation of linkage with differential viability. II and III. Heredity 3:220–228

  2. Barua UM, Chalmers KJ, Hackett CA, Thomas WTB, Powell W, Waugh R (1993) Identification of RAPD markers linked to Rhynchosporium secalis resistance locus in barley using nearisogenic lines and bulked segregant analysis. Heredity 71:177–184

  3. Chaparro JX, Werner DJ, O'Malley D, Sederoff RR (1994) Targeted mapping and linkage analysis of morphological isozyme, and RAPD markers in peach. Theor Appl Genet 87:805–815

  4. Delourme R, Bouchereau A, Hubert N, Renard M, Landry BS (1994) Identification of RAPD markers linked to a fertility restorer gene for the Ogura radish cytoplasmic male sterility of rapeseed (Brassica napus L.). Theor Appl Genet 88:741–748

  5. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissues. Focus 12:13–15

  6. Foisset N, Delourme R (1995) Segregation distortion in androgenicplants. In: Mohan Jain S, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants. Chapter 9, vol 2. Kluwer Academic, Dordrecht, Netherlands (in press)

  7. Harper FR, Berkenkamp B (1975) Revised growth-stage key for Brassica campestris and B. napus. Can J Plant Sci 57:657–659

  8. Islam N, Evan EJ (1994) Influence of lodging and nitrogen rate on the yield and yield attibutes of oilseed rape (Brassica napus L.). Theor Appl Genet 88:530–534

  9. Kesseli R, Witsenboer H, Stanghellini M, Vandermark G, Michelmore R (1993) Recessive resistance to Plasmopara lactucae-radicis maps by bulked segregant analysis to a cluster of dominant disease resistance genes in lettuce. Mol Plant-Microbe Interact 6:722–728

  10. Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eug 12:172–175

  11. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg, I (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

  12. Landry BS, Hubert N, Etoh T, Harada JJ, Lincoln SE (1991): A genetic map for Brassica napus based on restriction fragment length polymorphisms detected with expressed DNA sequences. Genome 34:543–552

  13. Laurie DA, Pratchett N, Romero C, Simpson E, Snape JW (1993) Assigment of the denso dwarfing gene to the long arm of chromosome 3(3H) of Barley by the use of RFLP markers. Plant Breed 111:198–203

  14. Lefebvre V, Chèvre AM (1995) Tools for marking plant disease and pest resistance genes: a review. Agronomie 15:3–19

  15. Liang CZ, Gu MH, Pan XB, Liang GH, Zhu LH (1994) RFLP tagging of a new semidwarfing gene in rice. Theor Appl Genet 88:898–900

  16. Lincoln S, M Daly, E Lander (1992) Constructing genetic maps with MAPMAKER/EXP 3.0. Whitehead Institute Techn Rep 3rd edn.

  17. Mather K (1957) The measurement of linkage in heredity. Methuen, London

  18. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

  19. Muehlbauer GJ, Specht JE, Thomas-Compton MA, Staswick PE, Bernard RL (1988) Near-isogenic lines — a potential resource in the integration of conventional and molecular marker linkage maps. Crop Sci 28:729–735

  20. Paran I, Kesseli R, Michelmore R (1991) Identification of restriction-fragment-length-polymorphism and random amplified polymorphic DNA markers linked to downy mildew resistance genes in lettuce, using near-isogenic lines. Genome 34:1021–1027

  21. Polsoni L, Kott LS, Beversdorf WD (1988) Large scale microspore culture technique for mutation-selection studies in Brassica napus. Can J Bot 66:1681–1685

  22. Quiros CF, Hu J, This P, Chevre AM, Delseny M (1991) Development and chromosomal localization of genome-specific markers by polymerase chain reaction in Brassica. Theor Appl Genet 82:627–632

  23. Stam P, Zeven AC (1981) The theoretical proportion of the donor genome in near-isogenic lines of self-fertilizers bred by backcrossing. Euphytica 30:227–238

  24. Williams PH, Hill CH (1986) Rapid cycling populations of Brassica. Science 232:1385–1389

  25. Worland AJ, Sayers EJ, Börner A (1994) The genetics and breeding potential of Rht12, a dominant dwarfing gene in wheat. Plant Breed 113:187–196

  26. Young, ND, Zamir D, Ganal MW Tanksley SD (1988) Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to the TM-2a gene in tomato. Genetics 120:579–585

  27. Zanewich KP, Rood SB, Southworth CE, Williams PH (1991) Dwarf mutants of Brassica: responses to applied gibberellins and gibberellin content. J Plant Growth Regul 10:121–127

Download references

Author information

Additional information

Communicated by H. F. Linskens

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Foisset, N., Delourme, R., Barret, P. et al. Molecular tagging of the dwarf BREIZH (Bzh) gene in Brassica napus . Theoret. Appl. Genetics 91, 756–761 (1995). https://doi.org/10.1007/BF00220955

Download citation

Key words

  • Brassica napus
  • Dwarfing gene
  • Bulked segregant analysis
  • Near-isogenic lines
  • Genetic mapping
  • RAPD markers
  • RFLP markers