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RFLP and RAPD mapping of the sunflower Pl1 locus for resistance to Plasmopara halstedii race 1

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Abstract

The Pl1 locus in sunflower, Helianthus annuus L., conferring resistance to downy mildew, Plasmopara halstedii, race 1 has been located in linkage group 1 of the consensus RFLP map of the cultivated sunflower. Bulked segregant analyses were used on 135 plants of an F2 progeny from a cross between a downy mildew susceptible line, GH, and RHA266, a line carrying Pl1. Two RFLP markers and one RAPD marker linked to the Pl1 locus have been identified. The RFLP markers are located at 5.6 cM and 7.1 cM on either side of Pl1. The RAPD marker is situated at 43.7 cM from Pl1. The significance and applications of these markers in sunflower breeding are discussed.

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Communicated by J. W. Snape

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Mouzeyar, S., Roeckel-Drevet, P., Gentzbittel, L. et al. RFLP and RAPD mapping of the sunflower Pl1 locus for resistance to Plasmopara halstedii race 1. Theoret. Appl. Genetics 91, 733–737 (1995). https://doi.org/10.1007/BF00220951

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Key words

  • Helianthus annuus
  • Plasmopara halstedii
  • Disease resistance
  • Bulked segregant analysis
  • Molecular markers