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Immunocytochemical localisation of prolactin and growth hormone in the ovine pituitary

A morphological and quantitative study

Summary

Using the peroxidase-antiperoxidase immunocytochemical staining technique, prolactin and growth hormone cells have been identified and described in the ovine pituitary. The image analysing computer, Quantimet 720, was used to assess accurately the size range of the secretory granules in these cell types. The area size distributions of the prolactin and growth hormone granules are similar. An increased proportion of larger granules was observed in the prolactin cells post-partum. Serial sections stained alternately for prolactin or growth hormone confirmed that the cells contain either prolactin or growth hormone but not both.

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References

  1. Baker, B.L., Jaffe, R.B.: The genesis of cell types in the adenohypophysis of the human fetus as observed with immunocytochemistry. Amer. J. Anat. 143, 137–162 (1975)

  2. Baker, B.L., Yu, Y.: An immunocytochemical study of human pituitary mammotrophs from fetal life to old age. Amer. J. Anat. 148, 217–240 (1977)

  3. Dacheux, F., Dubois, M.P.: Ultrastructural localisation of prolactin, growth hormone and luteinising hormone by immunocytochenical techniques in the bovine pituitary. Cell Tiss. Res. 174, 245–260 (1976)

  4. Foster, C.L.: Relationship between ultrastructure and function in the adenohypophysis of the rabbit. In: Memoirs of the Society for Endocrinology No. 19. Subcellular organisation and function in endocrine tissues (H. Heller and K. Lederis, eds.). (1971)

  5. Martin-Comin, J., Robyn, C.: Comparative immunoenzymatic localisation of prolactin and growth hormone in human and rat pituitaries. J. Histochem. Cytochem. 24, 1012–1016 (1976)

  6. Mikami, S.: Light and electronmicroscopic investigations of six types of glandular cell of the bovine adenohypophysis. Z. Zellforsch. 105, 457–482 (1970)

  7. Mikami, S., Daimon, T.: Cytological and cytochemical investigations of the adenohypophysis of the sheep. Arch. Histol. Jap. 29, 427–445 (1968)

  8. Parsons, J.A., Erlandson, S.L.: Ultrastructural immunocytochemical localisation of prolactin in rat anterior pituitary by use of the unlabelled antibody enzyme method. J. Histochem. Cytochem. 22, 340–351 (1974)

  9. Petrali, J.P., Hinton, D.M., Moriarty, G.C., Sternberger, L.A.: The unlabeled antibody enzyme method of immunocytochemistry with and without peroxidase-antiperoxidase complex. J. Histochem. Cytochem. 22, 782–801 (1974)

  10. Smith, R.E., Farquhar, M.G.: Modulation in nucleoside diphosphatase activity of mammotrophic cells of rat adenohypophysis during secretion. J. Histochem. Cytochem. 18, 237 (1970)

  11. Sternberger, L.A.: Immunocytochemistry. Prentice-Hall Inc. (1974)

  12. Wallace, A.L.C., Ferguson, K.A.: The preparation of sheep growth hormone. J. Endocr. 26, 254–263 (1963)

  13. Zimmerman, E.A., Defendini, R., Frantz, A.G.: Prolactin and growth hormone in patients with pituitary adenomas: a correlative study of hormone in tumor and plasma by immunoperoxidase technique and radioimmunoassay. J. clin. Endocr. 38, 577–585 (1974)

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Correspondence to Dr. D. M. Parry.

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Parry, D.M., McMillen, I.C. & Willcox, D.L. Immunocytochemical localisation of prolactin and growth hormone in the ovine pituitary. Cell Tissue Res. 194, 327–336 (1978). https://doi.org/10.1007/BF00220399

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Key words

  • Prolactin
  • Growth hormone
  • Ovine pituitary
  • Immunocytochemistry
  • Granule size