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Immunohistochemical localisation of ecdysteroids in the follicular epithelium of locust oocytes

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Summary

Frozen sections of growing terminal follicles of the locust ovary were incubated with an ecdysteroid-specific rabbit antibody and the bound antibody visualised by the use of FITC-labelled goat-anti-rabbit antiserum. A bright fluorescence was seen in the cytoplasm of the follicle cells in terminal follicles with a length between 4.0 and 6.0mm with a maximum intensity at 5.5mm, indicating the presence of ecdysteroids in these cells in this particular developmental stage.

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Correspondence to Prof. Dr. Hans Emmerich.

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Glass, H., Emmerich, H. & Spindler, K. Immunohistochemical localisation of ecdysteroids in the follicular epithelium of locust oocytes. Cell Tissue Res. 194, 237–244 (1978). https://doi.org/10.1007/BF00220391

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Key words

  • Ecdysteroids
  • Insect hormones
  • Immunohistochemistry
  • Insect ovary
  • Locusta