Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Immunohistochemical localisation of ecdysteroids in the follicular epithelium of locust oocytes

  • 33 Accesses

  • 24 Citations


Frozen sections of growing terminal follicles of the locust ovary were incubated with an ecdysteroid-specific rabbit antibody and the bound antibody visualised by the use of FITC-labelled goat-anti-rabbit antiserum. A bright fluorescence was seen in the cytoplasm of the follicle cells in terminal follicles with a length between 4.0 and 6.0mm with a maximum intensity at 5.5mm, indicating the presence of ecdysteroids in these cells in this particular developmental stage.

This is a preview of subscription content, log in to check access.


  1. Bassemir, U.: Ultrastructural differentiations in the developing follicle cortex of Locusta migratoria, with special reference to vitelline membrane formation. Cell Tiss. Res. 185, 247–262 (1977)

  2. Beckers, C., Emmerich, H.: Preparation of an ecdysone-6-carboxymethoxyloxime complex with bovine serum albumin. Naturwissenschaften 60, 50 (1973)

  3. Borst, D.W., O'Connor, J.D.: Arthropod molting hormone: A radioimmunoassay. Science 178, 418–419 (1972)

  4. Dennis, R.D.: Insect morphogenetic hormones and developmental mechanisms in the nematode, Nematospiroides dubius. Comp. Biochem. Physiol. 53A, 53–56 (1976)

  5. DeReggi, M.L., Hirn, M.H., Delaage, M.A.: Radioimmunoassay of ecdysone and application to Drosophila larvae and pupae. Biochem. biophys. Res. Commun. 66, 1307–1311 (1975)

  6. Gellissen, G., Emmerich, H.: Changes in the titer of vitellogenin and of diglyceride carrier lipoprotein in the blood of adult Locusta migratoria. Insect Biochem. (in press) (1978)

  7. Goldman, M: Fluorescent antibody methods. Academic Press New York: (1968)

  8. Goltzené, F.: Contribution à l'étude de l'ovogenèse chez Locusta migratoria (Orthoptère). Université Louis Pasteur, Strasbourg, Thèse (1977)

  9. Goodfriend, L., Leznoff, A., Sehon, A.H.: Antibodies to estrone-protein conjugates. III. Tissue localization of estrogen. Canad. J. Biochem. Physiol. 39, 967–971 (1961)

  10. Goodman, D.P.B., Bloom, F.E., Battenberg, E.R., Rasmussen, H., Davis, W.L.: Immunofluorescent localization of cyclic AMP in toad urinary bladder: Possible intercellular transfer. Science 188, 1023–1025 (1975)

  11. Harboe, N., Ingild, A.: Immunization, isolation of immunoglobulins, estimation of antibody titre. In: A manual of quantitative immunoelectrophoresis. (Axelsen, N.H., J. Krøll and B. Weeke eds.). Scand. J. Immunol. 2, Suppl. 1. Oslo: Universitetsforlaget 1973

  12. Herman, W.M.S.: Molt initiation in response to phytoecdysones and low doses of animal ecdysones in the chelicerate arthropod, Limulus polyphemus. Gen. comp. Endocr. 18, 301–305 (1972)

  13. Horn, D.H.S., Wilkie, J.S., Sage, B.A., O'Connor, J.D.: A high affinity antiserum specific for the ecdysone nucleus. J. Insect Physiol. 22, 901–905 (1976)

  14. Kagen, L.J., Gurevich, R.: Localization of myoglobin in human skeletal muscle using fluorescent antibody techniques. J. Histochem. Cytochem. 15, 436–441 (1967)

  15. Krishnakumaran, A., Schneiderman, H.: Control of molting in mandibulate and chelicerate arthropods by ecdysones. Biol. Bull. 139, 520–538 (1970)

  16. Lagueux, M.: Recherches sur l'ecdysone présente dans les ovaires adultes de Locusta migratoria: biosynthèse, destinée, fonctions. Thèse, Strasbourg (1978)

  17. Lagueux, M., Hirn, M., Hoffmann, J.A.: Ecdysone during ovarian development in Locusta migratoria. J. Insect Physiol. 23, 109–119 (1977)

  18. Lauer, R.C., Solomon, P.H., Nakanishi, K., Erlanger, B.F.: Antibodies to the insect moulting hormone β-ecdysone. Experientia (Basel) 30, 560–562 (1974)

  19. Maróy, P., Varga, J., Horváth, K.: Rapid heterologous haptene radioimmunoassay for insect moulting hormone. FEBS Letters 81, 2, 319–322 (1977)

  20. Mirecka, J.: Application of the anti-progesterone antibody for immunohistochemical demonstrations of progesterone. Acta histochem. (Jena) 51, 205–213 (1974)

  21. Mirecka, J.: Immunofluorescent localization of the female sex steroids in the porcine ovary. Histochem. J. 7, 249–257 (1975)

  22. Mordue, W., Goldsworthy, G.L.: The physiological effects of corpus cardiacum extracts in locusts. Gen. comp. Endocr. 12, 360–369 (1969)

  23. Nenci, I., Beccati, M.D., Piffanelli, A., Lanza, G.: Detection and dynamic localization of estradiol-receptor complexes in intact target cells by immunofluorescence technique. J. Steroid Biochem. 7, 505–510 (1976)

  24. Nenci, I., Piffanelli, A., Beccati, M.D., Lanza, G.: In vivo and in vitro immunofluorescent approach to the physiopathology of estradiol kinetics in target cells. J. Steroid Biochem. 7, 883–891 (1976)

  25. Ong, S.H., Whitley, T.H., Stowe, N.W., Steiner, A.L.: Immunohistochemical localization of 3′–5′-cyclic AMP and 3′–5′-cyclic GMP in rat liver, intestine and testis. Proc. Natl. Acad. Sci. U.S. 72, 2022–2026 (1975)

  26. Pertschuk, L.P.: Detection of estrogen binding in human mammary carcinoma by immunofluorescence: A new technique utilizing the binding hormone in a polymerized state. Res. Comm. Chen. Path. Pharmacol. 14, 771–774 (1976)

  27. Pollak, W.: Untersuchungen zur quantitativen Wirkung von Ecdysteron auf die postembryonale Morphogenese des Chilopoden Lithobius forficatus (L.). Zool. Jb. Physiol. 81, 383–394 (1977)

  28. Porcheron, P., Fourier, J., Gros, U., Pradelles, P., Cassier, P., Dray, F.: Radioimmunoassay of arthropod moulting hormone: β-ecdysone antibodies production and 125J iodinated tracer preparation. FEBS Letters 61, 159–163 (1976)

  29. Rosenberg, J., Seifert, G.: Ist allein die Glandula ecdysalis die Häutungsdrüse von Lithobius? Experientia (Basel) 31, 1100 (1975)

  30. Samoiloff, M.R.: Nematode morphogenesis: Localization of controlling regions by laser microbeam surgery. Science 180, 976–977 (1973)

  31. Scheffel, H., Wilkie, S., Pollak, W.: Die Wirkung von exogenem Ecdysteron auf Larven des Chilopoden Lithobius forficatus. Acta entomol. bohemoslov. 71, 233–238 (1974)

  32. Seifert, G., Rosenberg, J.: Elektronenmikroskopische Untersuchungen der Häutungsdrüsen (“Lymphstränge”) von Lithobius forficatus L. (Chilopoda). Z. Morph. Tiere 78, 263–279 (1974)

  33. Spindler, K.-D., Beckers, C., Gröschel-Stewart, U., Emmerich, H.: A radioimmunoassay for arthropod molting hormones introducing a novel method of immunogen coupling. Hoppe-Seylers Z. physiol. Chem. (accepted for publication) (1978)

  34. Vandesande, F., Dierickx, K.: Immunocytochemical demonstrations of separate vasotocinergic and mesotocinergic neurons in the amphibian hypothalamic magnocellular neurosecretory system. Cell Tiss. Res. 175, 289–296 (1976)

  35. Whitehead, D.L.: Steroid involvement in calcification of the invertebrate exoskeleton. J. Endocr. 61, 77–78 (1974)

  36. Whitehead, D.L.: Steroids enhance shell regeneration in an aquatic gastropod (Biomphalaria glabrata). Comp. Biochem. Physiol. 58C, 137–141 (1977)

  37. Woods, J.E., Domm, L.V.: A histochemical identification of the androgen-producing cells in the gonads of the domestic fowl and albino rat. Gen. Comp. Endocr. 7, 559–570 (1966)

Download references

Author information

Correspondence to Prof. Dr. Hans Emmerich.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Glass, H., Emmerich, H. & Spindler, K. Immunohistochemical localisation of ecdysteroids in the follicular epithelium of locust oocytes. Cell Tissue Res. 194, 237–244 (1978). https://doi.org/10.1007/BF00220391

Download citation

Key words

  • Ecdysteroids
  • Insect hormones
  • Immunohistochemistry
  • Insect ovary
  • Locusta