Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Oogenesis in the malaria mosquito Anopheles gambiae

  • 103 Accesses

  • 9 Citations

Summary

Oogenesis has been followed with the electron microscope in 2 strains of the malaria mosquito Anopheles gambiae, from the emergence of the adult (oocytes at leptonema) till shortly before the oocytes are ready for oviposition. After pachynema the chromosomes form a karyosphere and a fibrous capsule develops around it. Work on other mosquitoes suggests that the capsule may be related to the synaptonemal complexes. Both Anopheles strains contain at some time an extrachromosomal (not DNA-containing) body comparable to the karyosphere in size. Clusters of granules are present at the surface of the nucleolus and free in the nucleoplasm. Tentative results indicate that they may contain DNA. During oogenesis the nucleolus becomes very large, mainly because of proliferation of the nucleolonema. Towards the end of oocyte development the nucleus assumes the large canoe-shape also seen in Aedes and Culex. Nucleolonema traverse the entire nucleus, and modified granular clusters are found throughout.

This is a preview of subscription content, log in to check access.

References

  1. Anderson, W.A., Spielman, A.: Permeability of the ovarian follicle of Aedes aegypti mosquitoes. J. Cell Biol. 50, 201–221 (1971)

  2. Anderson, W.A., Spielman, A.: Incorporation of RNA and protein precursors by ovarian follicles of Aedes aegypti mosquitoes. J. Submicr. Cytol. 5, 181–198 (1973)

  3. Bauer, H.: Die wachsenden Oocytenkerne einiger Insekten in ihrem Verhalten zur Nuklealfärbung. Z. Zellforsch. 18, 254–298 (1933)

  4. Bier, K., Kunz, W., Ribbert, D.: Struktur und Funktion der Oocytenchromosomen und Nukleolen sowie der Extra-DNS während der Oogenese panoistischer und meroistischer Insekten. Chromosoma (Berl.) 23, 214–254 (1967)

  5. Bouteille, M., Laval, M., Dupuy-Coin, A.M.: Localization of nuclear functions as revealed by ultrastructural autoradiography and cytochemistry. In: The cell nucleus (ed. H. Busch), vol. 1. New York: Academic Press 1974

  6. Büning, J.: Untersuchungen am Ovar von Bruchidius obtectus Say. (Coleoptera-Polyphaga) zur Klärung des Oocytenwachstums in der Prävitellogenese. Z. Zellforsch. 128, 214–282 (1972)

  7. Clements, A.N.: The physiology of mosquitoes. New York: Pergamon Press 1963

  8. Davidson, G., Paterson, H.E., Coluzzi, M., Mason, G.F., Micks, D.W.: The Anopheles gambiae complex. In: Genetics of insect vectors of disease (ed. J.W. Wright and R. Pal). Amsterdam: Elsevier 1967

  9. Fiil, A.: Structural and functional modifications of the nucleus during oogenesis in the mosquito Aedes aegypti. J. Cell Sci. 14, 51–67 (1974)

  10. Fiil, A., Moens, P.B.: The development, structure and function of modified synaptonemal complexes in mosquito oocytes. Chromosoma (Berl.) 41, 37–62 (1973)

  11. Gall, J.G., MacGregor, H.C., Kidston, M.E.: Gene amplification in the oocytes of Dytiscid water beetles. Chromosoma (Berl.) 26, 169–187 (1969)

  12. Gruzova, M.N., Zaichikova, Z.P., Sokolov, I.I.: Functional organization of the nucleus in the oogenesis of Chrysopa perla L. (Insecta, Neuroptera). Chromosoma (Berl.) 37, 353–386 (1972)

  13. Huebner, E., Tobe, S.S., Davey, K.G.: Structural and functional dynamics of oogenesis in Glossina austeni: General features, previtellogenesis and nurse cells. Tissue Cell 7, 297–317 (1975)

  14. Jaworska, H., Lima-de-Faria, A.: Amplification of ribosomal DNA in Acheta. VI. Ultrastructure of two types of nucleolar components associated with ribosomal DNA. Hereditas (Lund.) 74, 169–186 (1973)

  15. Kato, K.: Cytochemistry and fine structure of elimination chromatin in Dytiscidae. Exp. Cell Res. 52, 507–522 (1968)

  16. Kinderman, N.B., King, R.C.: Oogenesis in Drosophila virilis. I. Interactions between the ring canal rims and the nucleus of the oocyte. Biol. Bull. 144, 331–354 (1973)

  17. Lima-de-Faria, A., Moses, M.J.: Ultrastructure and cytochemistry of metabolic DNA in Tipula. J. Cell Biol. 30, 177–192 (1966)

  18. Lima-de-Faria, A., Nilsson, B., Cave, D., Puga, A., Jaworska, H.: Tritium labelling and cytochemistry of extra DNA in Acheta. Chromosoma (Berl.) 25, 1–20 (1968)

  19. Nicholson, A.J.: The development of the ovary and ovarian egg of a mosquito, Anopheles maculipennis, Meig. Quart. J. micr. Sci. 65, 395–448 (1921)

  20. Roth, T.F.: Changes in the synaptinemal complex during meiotic prophase in mosquito oocytes. Protoplasma (Wien) 61, 346–386 (1966)

  21. Westergaard, M., Wettstein, D. von: Studies on the mechanism of crossing over. IV. The molecular organisation of the synaptinemal complex in Neottiella (Cooke) Saccardo (Ascomycetes). C.R. Lab. Carlsberg 37, 239–268 (1970)

  22. Woodruff, R.I., Telfer, W.H.: Polarized intercellular bridges in ovarian follicles of the Cecropia moth. J. Cell Biol. 58, 172–188 (1973)

  23. Zaichikova, Z.P., Gruzova, M.N.: Structural and functional organization of the oocyte nucleus in Chrysopa (Neuroptera). III. Extrachromosomal DNA in early oogenesis. Translated from: Ontogenez 4, 595–602 (1973)

Download references

Author information

Correspondence to Dr. Annelise Fiil.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Fiil, A. Oogenesis in the malaria mosquito Anopheles gambiae . Cell Tissue Res. 167, 23–35 (1976). https://doi.org/10.1007/BF00220157

Download citation

Key words

  • Oogenesis
  • Mosquito
  • Capsule
  • Nucleolus
  • Ultrastructure