Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Location of glycogen in spermatids and spermatozoa of the shipworm, Bankia australis (Teredinidae, Bivalvia, Mollusca)

Summary

The periodic acid-thiocarbohydrazide-silver proteinate technique and α-amylase digestion were used to locate glycogen in the spermatids and sperms of the bivalve Bankia australis. Glycogen was found in the middle piece and around the acrosome in spermatozoa, apparently randomly scattered throughout the cytoplasm of young spermatids, and in a cytoplasmic bead in old spermatids.

This is a preview of subscription content, log in to check access.

References

  1. Anderson, W.A.: Methods for electron microscopic localization of glycogen. Techniques of biochemical and biophysical morphology, (D. Glick and R.M. Rosenbaum, eds.), vol. 1; p. 1–23. New York: Wiley-Interscience 1971

  2. Anderson, W.A., Personne, P.: The localization of glycogen in the spermatozoa of various invertebrate species. J. Cell Biol. 44, 29–51 (1970)

  3. Beaulation, J.: Modifications ultrastructurales des cellules sécrétoires de la glande prothoracique de vers a soie au cours des deux derniers ages larvaires. II. Le glycogène, ses relations avec le chondriome et le reticulum endoplasmique. J. Microscopic (Paris) 7, 673–692 (1968)

  4. Firlet, C.F., Davies, J.R.: Morphogenesis of the residual body of the mouse testes. Quart. J. micr. Sci. 106, 93–98 (1965)

  5. Hayat, M.A.: Principles and techniques of electron microscopy. Biological Applications. Vol. 1, 412 pp. New York: Van Nostrand Reinhold Co. 1970

  6. Ledingham, J.N., Simpson, F.O.: Intensification of osmium staining by p-phenylenediamine; paraffin and epon embedding; lipid granules in renal medulla. Stain Technol. 45, 255–260 (1970)

  7. Longo, F.J., Anderson, E.: Sperm differentiation in the sea urchin Arbacia punctulata and Strongylocentrotus purpuratus. J. Ultrastruct. Res. 27, 86–509 (1969)

  8. Pearse, A.G.E.: Histochemistry: Theoretical and Applied. Vol. I, 3rd ed., 759 pp. London: Churchill 1968

  9. Pearse, A.G.E.: Histochemistry: Theoretical and Applied. Vol. II, 3rd ed., 760–1518 pp. London: Churchill 1972

  10. Popham, J.D.: Comparative morphometrics of the acrosomes of the sperms of “externally” and “internally“ fertilizing sperms of the shipworms (Teredinidae, Bivalvia, Mollusca). Cell Tiss. Res. 150, 291–297 (1974)

  11. Rebhun, L.I., Sander, G.: Electron microscopic studies of frozen substituted marine eggs. III: Structure of the mitotic apparatus of the first mitotic division. Amer. J. Anat. 130, 35–53 (1971)

  12. Rosa, F., Johnson, F.B.: Enzymatic digestion in the cytochemical demonstration of glycogen. J. Histochem. Cytochem. 15, 14–20 (1967)

  13. Thiery, J.P.: Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. J. Microscopie (Paris) 6, 987–1018 (1967)

Download references

Author information

Correspondence to Dr. J. D. Popham.

Additional information

The authors with to thank Associate Professor E. van Lennep of the Department of Histology and Embryology, University of Sydney, for his helpful comments on the preparation of the manuscript.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Popham, J.D., Dickson, M.R. Location of glycogen in spermatids and spermatozoa of the shipworm, Bankia australis (Teredinidae, Bivalvia, Mollusca). Cell Tissue Res. 164, 519–524 (1975). https://doi.org/10.1007/BF00219942

Download citation

Key words

  • Spermiogenesis
  • Bankia australis
  • Glycogen