Using the immunoenzyme bridge-technique at the light and electron microscopic levels, somatostatin can be demonstrated in the perikarya of the anterior periventricular nucleus, in the median eminence and in the parvocellular hypothalamic nuclei of the rat. In the latter regions the perikarya are negative, whereas a positive reaction for somatostatin is found in a delicate network of fibers and middle-sized granules of very small axons. In light of these results, the double function of somatostatin — as release inhibiting hormone and as transmitter — is discussed. The positive staining reaction in the organum vasculosum laminae terminalis of male and female rats as well as in the subfornical organ, the nucleus dorsalis thalami and the nucleus medialis habenulae in female controls and pregnant rats is not due to somatostatin-containing structures, but partly to substance P and partly to a substance which could not be defined.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Alpert, L.C., Brawer, J.R., Patel, Y.C., Reichlin, S.: Somatostatinergic neurons in anterior hypothalamus: Immunohistochemical localization. Endocrinol. 98, 255–258 (1976)
Arimura, A., Schally, A.V.: Increase in basal and thyrotropin-releasing hormone (TRH) stimulated secretion of thyrotropin (TSH) by passive immunization with antiserum to somatostatin in rats. Endocrinol. 98, 1069–1072 (1976)
Brownstein, M., Arimura, A., Sato, H., Schally, A.V., Kizer, J.S.: The regional distribution of somatostatin in the rat brain. Endocrinol. 96, 1456–1462 (1975)
Dubé, D., Leclerc, R., Pelletier, G., Arimura, A., Schally, A.V.: Immunohistochemical detection of growth hormone-release inhibiting hormone (somatostatin) in the guinea pig brain. Cell Tiss. Res. 161, 385–392 (1975)
Dubois, M.P.: Immunoreactive somatostatin is present in discrete cells of the endocrine pancreas. Proc. nat. Acad. Sci. (Wash.) 72, 1340–1343 (1975)
Efendic, S., Luft, R., Grill, V.: Effect of somatostatin on glucose induced insulin release in isolated perfused rat pancreas and isolated rat pancreatic islets. FEBS Letters 42, 169–172 (1974)
Elde, R.P., Parsons, J.A.: Immunocytochemical localization of somatostatin in cell bodies of the rat hypothalamus. Amer. J. Anat. 144, 541–548 (1975)
Goldsmith, P.C., Rose, J.C., Arimura, A., Ganong, W.F.: Ultrastructural localization of somatostatin in pancreatic islets of the rat. Endocrinol. 97, 1061–1064 (1975)
Hökfelt, T., Efendi, S., Hellerström, C., Johansson, O., Luft, R., Arimura, A.: Cellular localization of somatostatin in endocrine-like cells and neurons of the rat with special references to the A1-cells of the pancreatic islets and to the hypothalamus. Acta endocr. (Kbh.) 200, 5–41 (1975)
Hökfelt, T., Efendic, S., Johansson, O., Luft, R., Arimura, A.: Immunohistochemical localization of somatostatin (growth hormone release-inhibiting factor) in the guinea pig brain. Brain Res. 80, 165–169 (1974)
Hökfelt, T., Johansson, O., Fuxe, K., Löfström, A., Goldstein, M., Jeffcoate, S., Arimura, A.: Mapping and relationship of hypothalamic neurotransmitters and hypothalamic hormones. Proceedings of the 6th International Congress of Pharmacology, Vol. 3, 1976
King, J.C., Gerall, A.A., Fishback, J.B., Elkind, K.E., Arimura, A.: Growth hormone release-inhibiting hormone (GHRH) pathway of the rat hypothalamus revealed by the unlabeled antibody peroxidase-antiperoxidase method. Cell Tiss. Res. 160, 423–430 (1975)
Kizer, J.S., Palkovits, M., Brownstein, M.J.: Releasing factors in the circumventricular organs of the rat brain. Endocrinol. 98, 311–317 (1976)
Kizer, J.S., Palkovits, M., Tappaz, M., Kebabian, J., and Brownstein, M.J.: Distribution of releasing factors, biogenic amines, and related enzymes in the bovine median eminence. Endocrinol. 98, 685–695 (1976)
Krisch, B.: Immunohistochemical and electron microscopic study of the rat hypothalamic nuclei and cell clusters under various experimental conditions. Cell Tiss. Res. 174, 109–127 (1975)
Naik, D.V.: Immunohistochemical localization of LH-RH during different phases of estrus cycle of rat, with reference to the preoptic and arcuate neurons, and the ependymal cells. Cell Tiss. Res. 173, 143–166 (1976)
Palkovits, M., Brownstein, M.J., Arimura, A., Sato, H., Schally, A.V., Kizer, J.S.: Somatostatin content of the hypothalamic ventromedial and arcuate nuclei and the circumventricular organs in the rat. Brain Res. 109, 430–434 (1976)
Parsons, J.A., Erlandsen, S.L., Herge, O.D., McEvoy, R.C., Elde, R.: Central and peripheral localization of somatostatin immunoenzyme immunocytochemical studies. J. Histochem. Cytochem. 24, 872–882 (1976)
Pelletier, G., Leclerc, R., Dubé, D.: Immunohistochemical localization of hypothalamic hormones. J. Histochem. Cytochem. 24, 864–871 (1976)
Pelletier, G., Leclerc, R., Dubé, D., Labrie, F., Puviani, R., Arimura, A., Schally, A.V.: Localization of growth hormone-release-inhibiting hormone (somatostatin) in the rat brain. Amer. J. Anat. 142, 397–400 (1975)
Sétálò, G., Vigh, S., Schally, A.V., Arimura, A., Flerkò, B.: GH-RIH-containing neural elements in the rat hypothalamus. Brain Res. 90, 352–356 (1975)
Sternberger, L.A.: Immunocytochemistry. Foundation of Immunology Series (A. Osler, L. Weis, eds.), Englewood Cliffs, New Jersey: Prentice Hall Inc. 1974
Straus, W.: Inhibition of peroxidase by methanol and by methanol-nitroferricyanid for use in immunoperoxidase procedures. J. Histochem. Cytochem. 19, 682–688 (1971)
Vandesande, F., Dierickx, K.: Identification of the vasopressin producing and of the oxytocin producing neurons in the hypothalamic magnocellular neurosecretory system in the rat. Cell Tiss. Res. 164, 153–162 (1975)
Supported by the Deutsche Forschungsgemeinschaft (Grant Nr. Kr. 569/1) and Stiftung Volkswagenwerk
About this article
Cite this article
Krisch, B. Morphological equivalent of the bifunctional role of somatostatin. Cell Tissue Res. 179, 211–224 (1977). https://doi.org/10.1007/BF00219797
- Somatostatin (rat)
- Fibers and perikarya