Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Fine-structural characteristics of the liver of the cod (Gadus morhua macrocephalus), with special regard to the concept of a hepatoskeletal system formed by Ito cells

  • 59 Accesses

  • 12 Citations

Summary

The liver of the adult cod (teleost, Gadus morhua macrocephalus) was observed with transmission and scanning electron microscopy. Hepatocytes of this animal are extremely large (about 50–70 μm in diameter) and characterized by numerous large lipid droplets (5–15 μm in diameter) showing fluorescence for vitamin A, though weaker than that of Ito cells. No Kupffer cells were recognized in the endothelial lining. Collagen fibrils are sparse in the perisinusoidal space, while Ito cells stretching their long cytoplasmic processes to the perisinusoidal as well as interparenchymatous spaces are frequent. There are a number of large desmosomes between the cytoplasmic processes, and all Ito cells seem to be interconnected by these junctions. The cytoplasm in the cell bodies and cell processes is occupied by bundles of intermediate filaments, while organelles are poorly developed. Small vesicles and caveolae are arranged along the plasma membrane. Scanning electron microscopy shows distinct three-dimensional networks consisting of Ito cells and their processes, which might be supporting elements of the liver tissue. We wish to emphasize the concept of this hepatoskeletal system.

This is a preview of subscription content, log in to check access.

References

  1. Desaki J, Fujiwara T, Komuro T (1984) A cellular reticulum of fibroblast-like cells in the rat intestine: scanning and transmission electron microscopy. Arch Histol Jpn 47:179–186

  2. Fujita H (1985) On the Ito cell of the cod liver. Monbusho Kagakukenkyuhi Hojokin (Sogo A, NO.58370001) Kenkyu Seika Hokokusho S. Shibasaki (ed), (in Japanese), pp 18–21

  3. Fujita H, Tamaru T, Miyagawa J (1980) Fine structural characteristics of the hepatic sinusoidal walls of the goldfish (Carassius auratus). Arch Histol Jpn 43:265–273

  4. Ito T (1951) Cytological studies on stellate cells of Kupffer and fat-storing cells in the capillary wall of the human liver. Acta Anat Nippon 26:42

  5. Murakami T (1973) A metal impregnation method of biological specimens for scanning electron microscopy. Arch Histol Jpn 35:323–326

  6. Nopanitaya W, Aghajanian J, Grisham WJ, Carson LJ (1979) An ultrastructural study on a new type of hepatic perisinusoidal cell in fish. Cell Tissue Res 198:35–42

  7. Sakano E, Fujita H (1982) Comparative aspects on the fine structure of the teleost liver. Okajimas Folia Anat Japon 58:501–520

  8. Tanaka K, Naguro T (1981) High resolution scanning electron microscopy of cell organelles by a new specimen preparation method. Biomed Res 2 suppl:63–70

  9. Tanuma Y, Ito T (1980) Electron microscopic study on the sinusoidal wall of the liver of the crucian, Carassius carassius, with special remarks on the fat-storing cell (FSC). Arch Histol Jpn 43:241–263

  10. Tanuma Y, Ohata M, Ito T (1982) Electron microscopic study on the sinusoidal wall of the liver in the flatfish, Kareius bicoloratus: demonstration of numerous desmosomes along the sinusoidal wall. Arch Histol Jpn 45:453–472

Download references

Author information

Correspondence to Prof. Hisao Fujita.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Fujita, H., Tatsumi, H., Ban, T. et al. Fine-structural characteristics of the liver of the cod (Gadus morhua macrocephalus), with special regard to the concept of a hepatoskeletal system formed by Ito cells. Cell Tissue Res. 244, 63–67 (1986). https://doi.org/10.1007/BF00218382

Download citation

Key words

  • Cod liver
  • Hepatocyte
  • Ito cell
  • Desmosomes
  • Hepatoskeleton