Thioredoxin and thioredoxin reductase (NADPH-oxidized thioredoxin oxidoreductase, E.C. 22.214.171.124) have been proposed to be involved in several thioldependent reduction-oxidation reactions in cells. Both proteins have been immunohistochemically demonstrated in the periphery of the cytoplasm and in cytoplasmic granules of acinar and islet cells in mouse pancreas. In animals fed ad libitum, the staining for thioredoxin was more intense in the exocrine acinar cells than in the islet cells, whereas that for thioredoxin reductase was more intense in the endocrine than in the exocrine pancreas. In the islets of fed mice all endocrine cell types showed about the same staining intensity for thioredoxin, while thioredoxin reductase was greatly enriched in the somatostatin-containing D cells. Starvation overnight caused an increased staining for both proteins in the acinar cells as well as in the islets. Under conditions of starvation, thioredoxin reductase, in contrast to thioredoxin, appeared to increase preferentially in the islet B cells, as compared with the D cells. Cysteamine treatment reduced the staining for somatostatin and for thioredoxin reductase in the D cells without any obvious effect on the other pancreatic cells. The results are compatible with a role for thioredoxin and thioredoxin reductase in secretion.
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Ammon HPT, Grimm A, Lutz S, Wagner-Teschner D, Händel M, Hagen I (1980) Islets glutathione and insulin release. Diabetes 29:830–834
Cooperstein SJ, Watkins D (1977) Effect of alloxan on islet tissue permeability: protection and reversal by NADPH. Biochem Biophys Res Commun 79:756–762
Efendic S, Enzmann F, Nylén A, Uvnäs-Wallensten K, Luft R (1979) Effects of glucose/sulfonylurea interaction on release of insulin, glucagon, and somatostatin from isolated, perfused rat pancreas. Proc Natl Acad Sci (USA) 76:5901–5904
Grankvist K, Holmgren A, Luthman M, Täljedal IB (1982) Thioredoxin and thioredoxin reductase in pancreatic islets may participate in diabetogenic free-radical production. Biochem Biophys Res Commun 107:1412–1418
Hansson HA, Rozell B, Stemme S, Engström Y, Thelander L, Holmgren A (1985 a) Different cellular distribution of thioredoxin and subunit MI of ribonucleotide reductase in rat tissues. Exp Cell Res 163:363–369
Hansson HA, Holmgren A, Rozell B, Stemme S (1985 b) Thioredoxin and thioredoxin reductase form parts in a gate system regulating the epithelial cells of the small intestines — immunohistochemical studies. (Submitted)
Hansson HA, Heiander H, Holmgren A, Rozell B, Stemme S (1985 c) Distribution and functional changes of thioredoxin and thioredoxin reductase in the gastric mucosa — immunohistochemical studies. (Submitted)
Hellman B, Idahl LÅ, Lernmark Å, Sehlin J, Täljedal IB (1974) Membrane sulphydryl groups and the pancreatic B-cell recognition of insulin secretagogues. Excerpta Int Congr Ser 312:65–78
Holmgren A (1977) Bovine thioredoxin system. Purification of thioredoxin reductase from calf liver and thymus and studies of its function in disulfide reduction. J Biol Chem 252:4600–4606
Holmgren A (1979 a) Reduction of disulfides by thioredoxin: exceptional reactivity of insulin and suggested functions of thioredoxin in mechanism of hormone action. J Biol Chem 254:9113–9119
Holmgren A (1979 b) Thioredoxin catalyses the reduction of insulin disulfides by dithiothreitol and dihydrolipoamide. J Biol Chem 254:9627–9632
Holmgren A (1980) Pyridine nucleotide-disulfide oxido-reductases. In: Jeffery J (ed) Dehydrogenases requiring nicotinamide coenzymes, Birkhauser Verlag, Basel, pp 149–180
Holmgren A (1985) Thioredoxin. Ann Rev Biochem 54:237–271
Holmgren A, Luthman M (1978) Tissue distribution and subcellular localization of bovine thioredoxin determined by radioimmunoassay. Biochemistry 17:4071–4077
Holmgren A, Lyckeborg C (1980) Enzymatic reduction of alloxan by thioredoxin and NADPH-thioredoxin reductase. Proc Natl Acad Sci (USA) 77:5149–5152
Johnson GD, Araujo de C. Nogueira GM (1981) A simple method of reducing the fading of immunofluorescence during microscopy. J Immunol Methods 43:349–350
Luthman M, Holmgren A (1982) Rat liver thioredoxin and thioredoxin reductase: purification and characterization. Biochemistry 21:6628–6633
Malaisse WJ (1982) Alloxan toxicity to the pancreatic B cell. Biochem Pharmacol 31:3527–3534
Malaisse WJ, Hutton JC, Kawazu S, Herchuelz A, Valverde I, Seuer A (1979) The stimulus-secretion coupling of glucose-induced insulin release XXXV. The limbs between metabolic and cationic events. Diabetologia 16:331–341
Rozell B, Hansson HA, Luthman M, Holmgren A (1985) Immunohistochemical localization of thioredoxin and thioredoxin reductase in adult rats. Eur J Cell Biol 38:79–86
Sorenson RL, Grouse LH, Elde RP (1983) Cysteamine blocks somatostatin secretion without altering the course of insulin or glucagon release. A new model for the study of islet function. Diabetes 32:377–379
Stemme S, Hansson HA, Holmgren A, Rozell B (1985) Axoplasmic transport of thioredoxin and thioredoxin reductase in rat sciatic nerve. Brain Res 359:140–146
Täljedal IB (1981) On insulin secretion. Diabetologia 21:1–17
Thelander L, Reichard P (1979) Reduction of ribonucleotides. Ann Rev Biochem 48:133–158
Watkins D, Cooperstein SJ, Fiel S (1979) Studies on the selectivity of alloxan for the B-cells of the islets of Langerhans: effect of pH on the in vitro action of alloxan. J Pharmacol Exp Ther 208:184–189
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Hansson, H.-., Holmgren, A., Rozell, B. et al. Immunohistochemical localization of thioredoxin and thioredoxin reductase in mouse exocrine and endocrine pancreas. Cell Tissue Res. 245, 189–195 (1986). https://doi.org/10.1007/BF00218100
- Thioredoxin reductase
- Pancreas, exocrine
- Pancreas, endocrine
- Insulin cells