In the rat nephron, alkaline phosphatase (ALP) was observed, with the light microscope, to be present on the brush border of the proximal tubule and in a small band of cells of the juxtaglomerular apparatus. With the electron microscope, ALP activity could not be demonstrated in the macula densa cells proper, but was seen for the first time in a narrow zone of cells interposed between the macula densa and the vascular pole of the renal corpuscule. The lead phosphate precipitates were precisely localized on the plasma membranes which form an intricate network of cytoplasmic interdigitations. Since ALP is known to be involved in some steps of phosphate transport, the present morphological data might be considered as an indication for a role of phosphate as a signal ion for the autoregulation of glomerular filtration.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Bell PD, Navar LG (1982) Cytoplasmic calcium in the mediation of macula densa tubuloglomerular feedback responses. Science 215:670–673
Borgers M, Thoné F (1975) The inhibition of alkaline phosphatase by L-p-bromotetramisole. Histochemistry 44:277–280
Brière N, Petitclerc C, Plante GE (1983) Presence of alkaline phosphatase and γ-glutamyl transpeptidase on the parietal layer of Bowman's capsule. Acta Histochem (in press)
Brunette MG, Chan M, Lebrun M (1981) Phosphatase activity along the nephron of mice with hypophosphatemic vitamin-D-resistant rickets. Kidney Int 20:181–187
Finegan RP (1964) Alkaline phosphatase in the juxtaglomerular apparatus of the rat. Nature 203:768–769
Haensly WE, Granger HJ, Morris AC, Cioppe C (1982) Proximal tubulelike epithelium in Bowman's capsule in spontaneously hypertensive rats. Am J Pathol 107:92–97
Jacobsen NO, Jargensen F, Thomsen AC (1967) On the localization of some phosphatases in three segments of the proximal tubules in the rat kidney. J Histochem Cytochem 15:456–469
Kaissling B, Kriz W (1982) Variability of intercellular spaces between macula cells: a transmission electron microscopic study in rabbits and rats. Kidney Int 22:Suppl 12, 9–17
Mayahara H, Hirano H, Saito T, Ogawa K (1967) The new lead citrate method for the ultracytochemical demonstration of activity of non-specific alkaline phosphatase (orthophosphoric monoester phosphohydrolase). Histochemistry 11:88–96
McComb RB, Bowes GN, Jr, Posen S (1979) Alkaline phosphatase. Plenum Press, NY, London, p 89
Navar LG, Bell PD, Burke TJ (1982) Role of a macula densa feedback mechanism as a mediator of renal autoregulation. Kidney Int 22:Suppl 12, 157–164
Petitclerc C, Plante GE (1981) Renal transport of phosphate: role of alkaline phosphatase. Can J Physiol Pharmacol 59:311–323
Plante GE, Sobh M, Brière N, Petitclerc C (1982) Autoregulation of glomerular filtration: role of distal delivery of phosphate. Clin Invest Medicine 5:45B
Thompson SW (1966) Selected histochemical and histopathological methods. Thomas CC, Springfield, Illinois, p 629
Venkatachalam MA, Bernard DB, Donohoe JF, Levinsky NG (1978) Ischemic damage and repair in the rat proximal tubule: differences among the S1, S2 and S3 segments. Kidney Int 14:31–49
Ward AM (1970) Tubular metaplasia in Bowman's capsule. Arch Pathol Lab Med 23:472–474
This investigation was supported by grant MA-4663 from the Medical Research Council of Canada. The technical help of Messrs. Denis and Marc Martel is gratefully acknowledged
About this article
Cite this article
Brière, N., Plante, G.E. & Petitclerc, C. Electron-microscopic demonstration of alkaline-phosphatase activity in the juxtaglomerular apparatus. Cell Tissue Res. 234, 147–151 (1983). https://doi.org/10.1007/BF00217408
- Alkaline phosphatase
- Glomerular filtration
- Juxtaglomerular apparatus
- Macula densa