An ultrastructural and morphometric analysis was made of the nucleolar components in pinealocytes of 40 male Fischer rats sampled at eight times in an LD 12∶12 photoperiod cycle. Comparisons of results from the eight times showed variation in estimated mean volume of the granular component of ±29%, and of the fibrillar component ±11%, in relation to daily means. Peaks in mean volume of total nucleolus and its granular component occurred at 1 h of light. Near maximal and minimal mean volumes of the fibrillar component both occurred during both light and dark. Fibrillar centers (nucleolar organizer regions) of different sizes were found at all sampling times. It is concluded that temporal patterns in 24-h changes in the nucleolar components are most prominent in the granular component, and are more complex than suggested by changes in total nucleolar size or mean dimensions, and than represented by a simple biphasic circadian rhythm. Examples of different stages in the migration of the granular component, and of possible sites of nucleo-cytoplasmic transfer of nucleolar material, are described.
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Busch H, Smetana K (1970) The nucleolus. Academic Press, New York London
Busch H, Ballal NR, Busch RK, Choi YC, Davis F, Goldknopf IL, Matsui SI, Rao MS, Rothblum LI (1978) The nucleolus, a model for analysis of chromatin controls. Cold Spring Harbor Symposia on Quantitative Biol 42:665–683
Cruz-Orive L-M (1976) Correction of stereological parameters from biased samples on nucleated particle phases. I. Nuclear volume fractions. J Microsc 106:1–18
De Robertis E (1954) The nucleo-cytoplasmic relationship and the basophilic substance (ergastoplasm) of nerve cells (electron microscope observations). J Histochem Cytochem 2:341–345
Fakan S (1971) Inhibition of nucleolar RNP synthesis by cycloheximide as studied by high resolution radioautography. J Ultrastruct Res 34:586–596
Fakan S, Puvion E (1980) The ultrastructural visualization of nucleolar and extranucleolar RNA synthesis and distribution. Int Rev Cytol 65:255–299
Goessens G (1976) The nucleolar fibrillar centres in various cell types in vivo or in vitro. Cell Tissue Res 173:315–324
Goessens G (1978) Nucleolar ultrastructure during reversible inhibition of RNA synthesis in chick fibroblasts cultivated in vitro. J Ultrastruct Res 65:83–89
Goessens G (1979a) Localization of nucleolus-organizing regions in interphase cells. Cell Tissue Res 200:159–161
Goessens G (1979b) Relations between fibrillar centres and nucleolus-associated chromatin in Ehrlich tumour cells. Cell Biol Int Reports 3:337–343
Goessens G, Lepoint A (1979) The nucleolus-organizing regions (NOR'S): recent data and hypotheses. Biol Cell 35:211–220
Kervorkian J, Wessel W (1959) So-called “nuclear pellets” (“Kernkugeln”) of pineocytes. A M A Arch Pathol 68:513–524
Krstić RV (1979) Ultrastructure of the mammalian cell. Springer, Berlin Heidelberg New York, pp 26–27
Mayhew TM (1979) Isolated peritoneal macrophages: component-biased sampling. In: Weibel ER (ed) Stereological methods. Academic Press, New York, pp 1:331–337
Mayhew TM, Cruz-Orive L-M (1973) Stereological correction procedures for estimating true volume proportions from biased samples. J Microsc 99:287–299
Mayhew TM, Cruz-Orive L-M (1975) Some stereological correction formulae with particular applications in quantitative neurohistology. J Neurol Sci 26:503–509
Pévet P (1977) On the presence of different populations of pinealocytes in the mammalian pineal gland. J Neural Transm 40:289–304
Quay WB (1963) Cytologic and metabolic parameters of pineal inhibition by continuous light in the rat (Rattus norvegicus). Z Zellforsch 60:479–490
Quay WB (1965) Histological structure and cytology of the pineal organ in birds and mammals. Prog Brain Res 10:49–86
Quay WB (1976) Seasonal cycle and physiological correlates of pinealocyte nuclear and nucleolar diameters in the bats, Myotis lucifugus and Myotis sodalis. Gen Comp Endocrinol 29:369–375
Quay WB (1978) Quatitative morphology and environmental responses of the pineal gland in the collared lemming (Dicrostonyx groenlandicus). Am J Anat 153:545–562
Quay WB, Renzoni A (1966) Twenty-four-hour rhythms in pineal mitotic activity and nuclear and nucleolar dimensions. Growth 30:315–324
Recher L, Syker JA, Chan H (1976) Further studies on the mammalian cell nucleolus. J Ultrastruct Res 56:152–163
Ripley BD (1981) Spatial statistics. John Wiley & Sons, New York
Royal A, Simard R (1975) RNA synthesis in the ultrastructural and biochemical components of the nucleolus. J Cell Biol 66:577–585
Satodate R, Sasou S, Ota M, Takashio M, Yoshida Y (1980) An electron microscopical study on pinealocytes of pregnant rats. Endokrinologie 75:285–291
Smetana K, Narayan K, Busch H (1966) Quantitative analysis of ultrastructural components of nucleoli of the Walker tumor and liver. Cancer Res 26:786–796
Studzinski GP, Gierthy JF (1972) Cytologic appearance of the nucleolus of normal and neoplastic cells in relation to the synthesis of RNA. Acta Cytol 16:245–248
Weibel ER, Bolender RP (1973) Stereological techniques for electron microscopic morphometry. In: Hayat MA (ed) Principles and techniques of electron microscopy. 3:237–296, Van Nostrand Rainhold, New York
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Lew, G.M., Payer, A. & Quay, W.B. The pinealocyte nucleolus. Cell Tissue Res. 224, 195–206 (1982). https://doi.org/10.1007/BF00217279
- Twenty-four-hour changes