The regeneration of skeletal muscle fibers of the adult chicken was examined after a focal injury brought about with a liquid-nitrogen cooled brass rod. Immunofluorescence microscopy with antibodies specific for troponin (TN) components (T, I, and C) from adult chicken breast and ventricular muscles showed the presence of different fiber types in both the anterior and posterior latissimus dorsi muscles. New fibers produced in the regions adjacent to the site of injury in both muscles exhibited the same immunoreactivities as those previously seen in embryonic skeletal muscles. As differentiation proceeded, regenerating cells lost their embryonic antigenicities and recovered their characteristic adult reactivities. These results indicate that, during regeneration from cold injury, skeletal muscles apparently pass again through an embryonic stage during which they synthesize embryonic-like TN isoforms.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Benfield PA, Lowey S, LeBlanc DD (1981) Fractionation and characterization of myosins from embryonic chicken pectoralis muscle. Biophys J 33:243a
Carlson BM (1973) The regeneration of skeletal muscle — a review. Am J Anat 137:119–150
Carraro U, Libera LD, Catani C (1983) Myosin light and heavy chains in muscle regenerating in absence of the nerve: transient appearance of the embryonic light chain. Exp Neurol 79:106–117
Devlin RB, Emerson CPJr (1978) Coordinate regulation of contractile protein synthesis during myoblast differentiation. Cell 13:599–611
Dhoot GK, Perry SV (1980) The components of the troponin complex and development in skeletal muscle. Exp Cell Res 127:75–87
Dhoot GK, Perry SV (1982) Changes in the forms of the components of the troponin complex during regeneration of injured skeletal muscle. Muscle Nerve 5:39–47
Gorza L, Sartore S, Triban C, Schiaffmo S (1983) Embryonic-like myosin heavy chains in regenerating chicken muscle. Exp Cell Res 143:395–403
Hall-Craggs ECB (1974) Rapid degeneration and regeneration of a whole skeletal muscle following treatment with bupivacaine (Marcain). Exp Neurol 43:349–358
Kelly AM, Rubinstein NA (1980) Patterns of myosin synthesis in regenerating normal and denervated muscles of the rat. In: Pette D (ed) Plasticity of muscle. Walter de Gruyter, Berlin New York, pp 161–175
Matsuda R, Obinata T, Shimada Y (1981) Types of troponin components during development of chicken skeletal muscle. Dev Biol 82:11–19
Miyazaki M, Toyota N, Shimada Y (1984) Distribution of polymorphic forms of troponin components in extra- and intrafusal fibers of an avian slow muscle. Cell Tissue Res 236:541–548
Rubinstein NA, Kelly AM (1981) Development of muscle fiber specialization in the rat hindlimb. J Cell Biol 90:128–144
Sartore S, Gorza L, Schiaffino S (1982) Fetal myosin heavy chains in regenerating muscle. Nature 298:294–296
Shimada Y, Obinata T (1977) Polarity of actin filaments at the initial stage of myofibril assembly in myogenic cells in vitro. J Cell Biol 72:777–785
Toyota N, Shimada Y (1981) Differentiation of troponin in cardiac and skeletal muscles in chicken embryos as studied by immunofluorescence microscopy. J Cell Biol 91:497–504
Toyota N, Shimada Y (1983) Isoform variants of troponin in skeletal and cardiac muscle cells cultured with and without nerves. Cell 33:297–304
Toyota N, Fischman DA, Masaki T, Shimada Y (1981) Isoform variants of troponin and myosin detected by immunofluorescence during muscle regeneration. J Cell Biol 91:352a
Whalen RG, Schwartz K, Bouveret P, Sell SM, Gros F (1979) Contractile protein isozymes in muscle development: identification of an embryonic form of myosin heavy chain. Proc Natl Acad Sci USA 76:5197–5201
About this article
Cite this article
Toyota, N., Shimada, Y. Isoforms of troponin during regeneration of chicken skeletal muscle fibers after cold injury. Cell Tissue Res. 236, 549–554 (1984). https://doi.org/10.1007/BF00217221
- Troponin-T, -I, and -C
- Skeletal muscle