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Oxidative changes in brain of aniline-exposed rats


Oxidative stress in rat cerebellum, cortex and brain stem after a short-term high-dose exposure to aniline vapors under conditions akin to those after major chemical accidents, was studied. Significant increases in superoxide dismutase isozyme activities and formation of thiobarbituric acid reactive material along with depletion of ascorbic acid and non-protein sulfhydryl content suggest impairment of antioxidant defenses 24 h after single exposure to 15,302 ppm aniline vapors for 10 min.

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  1. Awasthi S, Kakkar P, Viswanathan PN, Bhardwaj R (1989a) Effect of anaesthetic ether on lipid peroxidation and superoxide dismutase isozymes of young and adult rat brain. Ind J Exp Biol 27:647–649

  2. —, —, —, — (1989b) Effect of phenobarbitone on antioxidant mechanisms in the brain. Clin Chem Enzymol Commun 1:145–152

  3. —, —, —, — (1991) Increase in lysolecithin content of brain microsomes in phenobarbitone-administered rats and its relation to lipid peroxidation. J Neurochem 57:277–281

  4. Bellavite P (1988) The superoxide-forming enzymatic system of phagocytes. Free Radic Biol Med 4:225–261

  5. Braughler JM (1985) Lipid peroxidation induced inhibition of γ-aminobutyric acid uptake in rat brain synaptosomes: protection by glucocorticoids. J Neurochem 44:1282–1288

  6. Cadet JL, Lohr JB, Jeste PV (1986) Free radical and tardive dyskinesia. Trends Neurosci 9:107–108

  7. Comporti M (1989) Three models of free radical induced cell injury. Chem Biol Interact 72:1–56

  8. Fisher RA (1950) Statistical methods for research, 11th ed. Oliver and Boyd, Edinburgh, UK

  9. Gardner HW (1989) Oxygen radical chemistry of polyunsaturated fatty acids. Free Radic Biol Med 7:65–86

  10. Glowinski J, Iverson LL (1966) Regional studies of catecholamine in rat brain. J Neurochem 13:655–669

  11. Halliwell B, Gutteridge JMC (ed) (1985) Free radicals in biology and medicine. Clarendon Press, Oxford, p 107

  12. Imai Y, Ito A, Sato R (1966) Evidence for the biochemically different types of vesicle in hepatic microsomal fraction. J Biol Chem 60:417–428

  13. Jewett SL, Eddy LJ, Hochstein P (1989) Is the autoxidation of catecholamines involved in ischemia—reperfusion injury? Free Radic Biol Med 6:185–188

  14. Jones DP (1985) Role of oxygen concentration in oxidative stress: Hypoxic and hyperoxic model. In: Sies F (ed) Oxidative stress, Academic Press, NY, pp 152–189

  15. Kakkar P, Das B, Viswanathan PN (1984) A modified spectrophotometric assay of superoxide dismutase. Ind J Biochem Biophys 21:130–132

  16. — (1986) Superoxide dismutase isozymes of rat lung. Ind J Biochem Biophys 23:339–343

  17. —, — (1987) Induction of superoxide dismutase isozymes in rabbit lung due to aniline exposure. J Appl Toxicol 7:7–13

  18. —, — (1990) Superoxide dismutase isozymes and toxicity of environmental xenobiotics. In: Channa Reddy C, Hamilton GA and Madhyastha KM (eds), Biological oxidation systems, vol. 1, Academic Press, New York, p 527–534

  19. Kiese M (1976) The biochemical production of ferihemoglobin forming derivatives from aromatic amines and mechanism of ferihemoglobin formation. Pharmacol Rev 18:1091–1099

  20. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275

  21. Mitoma C, Ponsner HS, Reitz HC, Udenfriend S (1956) Enzymatic hydroxylation of aromatic amines. Arch Biochem Biophys 61: 431–441

  22. Mustafa MG (1974) Augmentation of mitochondrial oxidation in lung tissue during recovery of animal from acute ozone exposure. Arch Biochem Biophys 165:531–538

  23. Nishikimi M, Rao NA, Yagi K (1972) The occurrence of superoxide anion in the reaction of reduced PMS and molecular oxygen. Biochem Biophys Res Commun 46:849–854

  24. Ohkawa H, Ohishi N, Yagi K (1979) Assay of lipid peroxidation in animal tissue by thiobarbituric acid reaction. Anal Biochem 95:351–358

  25. Parke DV (1960) The metabolism of 14C aniline in rabbit and other animals. Biochem J 77:493–503

  26. Pestana A, Munoz E (1982) Anilides and Spanish toxic oil syndrome. Nature 298:608

  27. Roe JH, Kuether CA (1943) Ascorbic acid in whole blood and urine through the 2–4 dinitrophenyl hydrazine derivatives of dehydro ascorbic acid. J Biol Chem 147:399–407

  28. Sedlock Y, Lindsay RH (1968) Estimation of total, protein-bound and non-protein-bound—SH group in tissue with ellmanoic reagent. Anal Biochem 25:192–205

  29. Sevanian A, Nordenbrand K, Kim E, Ernster L, Hochstein P (1990) Microsomal lipid peroxidation: The role of NADPH-cytochrome P450 reductase and cytochrome P450. Free Radic Biol Med 8: 145–152

  30. Udenfriend S, Clark CT, Axelrod J, Brodie BB (1954) Ascorbic acid in aromatic hydroxylation. J Biol Chem 208:731–739

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Correspondence to P. N. Viswanathan.

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Kakkar, P., Awasthi, S. & Viswanathan, P.N. Oxidative changes in brain of aniline-exposed rats. Arch. Environ. Contam. Toxicol. 23, 307–309 (1992).

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  • Ascorbic Acid
  • Superoxide Dismutase
  • Thiobarbituric
  • Thiobarbituric Acid
  • Sulfhydryl