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Distribution of a galactose-specific lectin in endoderm cells from early chick embryos

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Summary

Cells from the endoderm of the area opaca of gastrulating chick embryos were maintained in stationary cultures, stained with antibodies against the endogenous β-D-galactoside-binding lectin and examined by immuno-fluorescence. In the majority of cells fluorescence was present as an irregular circular web in the central cytoplasm. In cells that appeared to be migrating increased fluorescence was observed in the peripheral cytoplasm and retraction fibers. In regions where a portion of the cell was detaching from the substratum high fluorescence was observed in the extracellular “footprints” deposited by the cell.

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References

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Correspondence to Dr. S. E. Zalik.

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Zalik, S.E., Milos, N. & Ledsham, I. Distribution of a galactose-specific lectin in endoderm cells from early chick embryos. Cell Tissue Res. 225, 223–228 (1982). https://doi.org/10.1007/BF00216231

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Key words

  • Lectin
  • Immunofluorescence
  • Adhesion
  • Morphogenetic movement