Cells from the endoderm of the area opaca of gastrulating chick embryos were maintained in stationary cultures, stained with antibodies against the endogenous β-D-galactoside-binding lectin and examined by immuno-fluorescence. In the majority of cells fluorescence was present as an irregular circular web in the central cytoplasm. In cells that appeared to be migrating increased fluorescence was observed in the peripheral cytoplasm and retraction fibers. In regions where a portion of the cell was detaching from the substratum high fluorescence was observed in the extracellular “footprints” deposited by the cell.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Beyer EC, Tokoyasu KT, Barondes SH (1979) Localization of an endogenous lectin in chicken liver, intestine and pancreas. J Cell Biol 82:565–571
Briles EB, Gregory W, Fletcher P, Kornfeld S (1979) Vertebrate lectins. Comparison of properties of β-galactoside-binding lectins from tissues of calf and chicken. J Cell Biol 81:528–537
Cook GMW, Zalik SE, Milos N, Scott V (1979) A lectin which binds specifically to β-D-galactoside groups is present at the earliest stages of chick embryo development. J Cell Sci 38:293–304
Garvey JS, Cremer NE, Sussdorf DH (1977) Methods in immunology. WA Benjamin, Massachusetts
Gremo F, Kobiler D, Barondes SH (1978) Distribution of an endogenous lectin in the developing chick optic tectum. J Cell Biol 79:491–499
Hamburger V, Hamilton HJ (1951) A series of normal stages in the development of the chick embryo. J Morph 88:49–92
Milos N, Zalik SE (1981a) Effect of the β-D-galactoside-binding lectin on cell to substratum and cell to cell adhesion of cells from the extraembryonic endoderm of the early chick blastoderm. Wilhelm Roux's Arch 190:259–266
Milos N, ZalikSE (1981b) Mechanisms of adhesion among cells of the early chick blastoderm. Role of the β-D-galactoside-binding lectin in the adhesion of extraembryonic endoderm cells. Differentiation (in press)
Milos N, Zalik SE, Phillips JR (1979a) An analysis of the aggregation and morphogenesis of area opaca endoderm cells from the primitive-streak chick embryo. J Emb Exp Morph 51:121–135
Milos N, Zalik SE, Veeken M-L (1979b) Inhibitors of the β-D-galactoside-binding lectin of the chick blastoderm affect the adhesion of extra-embryonic endoderm cells from chick blastoderms. J Cell Biol 83:68
Milos N, Zalik SE, Ledsham I, Stacey S (1980) The endogenous lectin of the primitive streak chick blastoderm decreases the adhesion of extraembryonic endoderm cells. Am Zool 20:802
Nowak TP, Kobiler D, Roel LE, Barondes SH (1977) Developmentally regulated lectin from embryonic chick pectoral muscle. J Biol Chem 252:6026–6030
Pannett CA, Compton A (1924) The cultivation of tissues in saline embryonic juice. Lancet 205:381–384
Phillips JR, Zalik SE (1980) Variations in lectin-mediated agglutination of trypsinized and nontrypsinized cells from two regions of the early chick embryo. Proc Can Fed Biol Soc 23:161
Phillips JR, Zalik SE (1981) Differential lectin-mediated agglutinabilities between the embryonic and first extra-embryonic cell line of the early chick embro. Wilhelm Roux's Arch (in press)
Podleski TR, Greenberg I (1980) Distribution and activity of endogenous lectin during myogenesis as measured with antilectin antibody. Proc Natl Acad Sci USA 77:1054–1058
Renn DW, Evans E (1975) Use of heteropoly acids as immunological precipitin brighteners. Anal Biochem 64:620–623
Romanoff A (1960) The avian embryo. MacMillan, New York
Zalik SE, Cook GMW (1976) Comparison of early embryonic and differentiating cell surfaces. Interaction of lectins with plasma membrane components. Biochem Biophys Acta 419:119–136
Zalik SE, Milos N, Ledsham I (1981) Distribution of two β-D-galactoside-binding lectins in the early chick blastoderm. Proc IXth Cong Intl Soc Dev Biol GII-5
Ziomek CA, Schulman S, Edidin M (1980) Redistribution of membrane proteins in isolated mouse intestinal epithelial cells. J Cell Biol 86:849–857
About this article
Cite this article
Zalik, S.E., Milos, N. & Ledsham, I. Distribution of a galactose-specific lectin in endoderm cells from early chick embryos. Cell Tissue Res. 225, 223–228 (1982). https://doi.org/10.1007/BF00216231
- Morphogenetic movement