Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Prenatal development of the rat dorsal root ganglia

A scanning electron-microscopic study

  • 81 Accesses

  • 20 Citations

Summary

This study describes three-dimensional aspects of the development and pseudo-unipolarization of neuroblasts and the maturation of satellite cells in prenatal rat dorsal root ganglia, using scanning electron microscopy, after removal of extracellular connective tissue components by trypsin digestion and HC1 hydrolysis.

At 14 days of gestation, the vast majority of neurons are spindle-shaped or bipolar and only 3% are unipolar, while at 16 and 18 days this percentage has increased to 30% and 91%, respectively. The initial portions of the central and peripheral neuronal processes gradually approach each other and form a common initial portion. Finally, the cytoplasm of this common initial portion becomes thinner and elongates to form the stem process of the mature cell.

Satellite cells are present from the beginning of the period studied, but intricate networks of branching satellite cell processes only develop after about day 17.

This is a preview of subscription content, log in to check access.

References

  1. Cajal S, Ramón y (1890) Sur l'origine et les ramifications des fibres nerveuses de la moelle embryonnaire. Anat Anz 5:85–95

  2. Cajal S, Ramón y (1893) Neue Darstellung vom histologischen Bau des Centralnervensystems. Arch Anat U Physiol Anat Abt 319–428

  3. Cajal S, Ramón y (1904) Asociacion del metodo del nitrato de plata con el embrionario. Para el estudio de los focos motores y sensitivos. Trab Lab Invest Biol Univ Madrid 3:65–96

  4. Cajal S, Ramón y (1907) Die Struktur der sensiblen Ganglien des Menschen und der Tiere. Ergeb Anat Entwickl Gesch 16:177–215

  5. Cajal S, Ramón y (1909) Histologie du système nerveux de l'homme et des vertébrés, vol l, Maloine, Paris

  6. Desaki J, Uehara Y (1981) The overall morphology of neuromuscular junctions as revealed by scanning electron microscopy. J Neurocytol 10:101–110

  7. Dogiel AS (1896) Der Bau der Spinalganglien bei den Säugetieren. Anat Anz 12:140–152

  8. Fujiwara T, Uehara Y (1981) Scanning electron microscopy of myenteric plexus: A preliminary communication. J Electron Microsc 29:397–400

  9. Hannover A (1844) Recherches microscopiques sur le système nerveux. Philipsen Brockhaus Avenarius Copenhagne

  10. His W (1886) Zur Geschichte des menschlichen Rückenmarks und der Nervenwurzeln. Abh math-phys Kl d Kgl sächs Ges Wiss 13:479–513

  11. His W (1887) Die Entwickelung der ersten Nervenbahnen beim menschlichen Embryo. Übersichtliche Darstellung. Arch Anat U Abt 368–378

  12. Lawson SN, Biscoe TJ (1979) Development of mouse dorsal root ganglia; an autoradiographic and quantitative study. J Neurocytol 8:265–274

  13. Lawson SN, Caddy KWT, Biscoe TJ (1974) Development of rat dorsal root ganglion neurons. Cell Tissue Res 153:399–413

  14. Lenhossék M (1892) Beobachtungen an den Spinalganglien und dem Rückenmark von Pristiurusembryonen. Anat Anz 7:519–539

  15. Lieberman AR (1976) Sensory Ganglia. In: Landon DN (ed) The peripheral nerve. Chapman and Hall, London, pp 188–278

  16. Matsuda S, Uehara Y (1981) Cytoarchitecture of the rat dorsal root ganglia as revealed by scanning electron microscopy. J Electron Microsc 30:136–140

  17. Murnaghan DP (1941) Studies on living spinal ganglion cells. Anat Rec 81:183–203

  18. Pannese E (1968a) Developmental changes of the endoplasmic reticulum and ribosomes in nerve cells of the spinal ganglia of the domestic fowl. J Comp Neurol 132:331–364

  19. Pannese E (1968b) Temporary junctions between neuroblasts in the developing spinal ganglia of the domestic fowl. J Ultrastruct Res 21:233–250

  20. Pannese E (1969) Electron microscopical study on the development of the satellite cell sheath in spinal ganglia. J Comp Neurol 135:381–422

  21. Pannese E (1974) The histogenesis of the spinal ganglia. Adv Anat Embryol Cell Biol 47:Fasc 5. Springer, Berlin Heidelberg New York

  22. Pannese E (1981) The satellite cells of the sensory ganglia. Springer, Berlin Heidelberg New York

  23. Retzius G (1891) Zur Kenntnis des centralen Nervensystems; quoted by Scharf JH. In: v. Möllendorffs Handbuch der mikroskopischen Anatomie des Menschen, vol IV, l, Sensible Ganglien. Springer, Berlin Göttingen Heidelberg 1958

  24. Rio Hortega P del, Polak M, Prado JM (1942) Investigaciones sobre la neuroglia de los ganglios sensitivos. Arch Histol (B. Aires) 1:233–275

  25. Tennyson V (1965) Developing sensory neuroblast. J Comp Neurol 124:267–318

  26. Uehara Y, Suyama K (1978) Visualization of the adventitial aspect of vascular smooth muscle cells under the scanning electron microscope. J Electron Microsc 27:157–159

  27. Yamadori T (1970) A light and electron microscopic study on the postnatal development of spinal ganglia in rats. Acta Anatomica Nippon 45:191–204

Download references

Author information

Correspondence to Seiji Matsuda.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Matsuda, S., Uehara, Y. Prenatal development of the rat dorsal root ganglia. Cell Tissue Res. 235, 13–18 (1984). https://doi.org/10.1007/BF00213717

Download citation

Key words

  • Spinal ganglia
  • Pseudounipolarization
  • Satellite cells
  • Histogenesis
  • SEM