Hyaluronic acid was partially sulfated and a polymer containing a precise number of sulfate groups for each repeating unit was prepared. The properties of this macromolecule in aqueous solution were studied at different pH levels by using potentiometric, viscosimetric techniques and were compared with that of the native hyaluronic acid, partially esterified hyaluronic acid and heparin. The influence of sulfate groups on the macromolecule is evident in the protonation constant and in the conformation of the polymer. This material exhibits an antithrombotic effect as evidenced by the lengthening of both the thrombin time and the whole blood clotting time. Moreover, the absence of hemolysis and the growth and shape of endothelial cells put in contact with the sulfated hyaluronic acid indicate that this polymer is a promising heparin-like compound.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
H. B.NADER, N. M.MCDUFFIE and C. P.DIETRICH, Biochem. Biophys. Res. Commun. 57 (1974) 488.
S. S.STIVALA and P.LIBERTI, Arch. Biochem. Biophys. 122 (1967) 40.
J.HOFFMAN, O.LARM and K.LARSSON, Carbohydrate Res. 2 (1982) 115.
G.KINDNESS, W. F.LONG and F. B.WILLIAMSON, Brit. J. Pharmac. 69 (1980) 675.
D.HORTON and E. K.JUST, Carbohydrate Res. 30 (1973) 349.
M.OKADA, H.SUMMIMOTO, M.HASEGAWA and K.KOMODA, Makromol. Chem. 180 (1979) 813.
Y.KIKUCHI and Y.OMISHI, Nippon Kagaku Kaishi 1 (1979) 127.
M.MANZAC, C.FOUGNOT and J.JOZEFOWICZ, Proc. Third M.I.S.A.O. 5 (1981) 504.
F. M.KANMAUGUE, D.LABARRE, H.SERNE and M.JOZEFOWICZ, Biomaterials 6 (1985) 297.
Y.ITO, Y.IGUCHI and Y.IMANISHI, Biomaterials, 13 (1992) 131.
K.NAGASAWA, H.UCHIYAMA and N.WAJIMA, Carboxhydrate Res. 158 (1986) 183.
R.BARBUCCI, M.CASOLARO and A.MAGNANI, Coord. Chem. Rev. 120 (1992) 29.
A.ALBANESE, R.BARBUCCI, R.ELOY, J.BELLEVILLE, S.BOWRY, H. D.LEMKE and L.SABATINI, Biomaterials 15 (1994) 129.
S. S.STIVALA, Fed. Proceed. 36 (1977) 83.
About this article
Cite this article
Barbucci, R., Benvenuti, M., Casolaro, M. et al. Sulfated hyaluronic acid as heparin-like material: physicochemical and biological characterization. J Mater Sci: Mater Med 5, 830–833 (1994). https://doi.org/10.1007/BF00213143
- Endothelial Cell