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Aerobic transformation of linear alkylbenzenesulphonates by mixed methane-utilizing bacteria

Abstract

Biodegradation of commercial linear alkylbenzenesulphonate (LAS) was performed in shake flasks in the presence and absence of methane as a primary growth substrate for methanotrophs and as an inducer for catabolic enzymes. Methane-utilizing culture MM1 was dominated by type II methanotrophs and originated from a groundwater aquifer. Methane, carbon dioxide, and oxygen concentrations were measured by using gas chromatography. Disappearance of LAS, a mixture of C10-C13 homologues, was monitored by reversed-phase high-performance liquid chromatography (RP-HPLC). Methane reduced LAS transformation rate in a concentration dependent manner, suggesting competitive inhibition between natural substrate, methane, and fortuitous substrate, LAS. The fastest LAS transformation was determined in the absence of methane. Simultaneous methane oxidation and LAS degradation, and the inhibition of both transformation processes by acetylene, indicated the involvement of a methane-monooxygenase enzyme system in LAS transformation. RP-HPLC analysis showed that culture MM1 exhibited preferential capability to transform LAS with longer alkyl side-chain. Consequently, the highest transformation rate was observed with the homologue of C13 LAS.

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References

  1. Alvarez-Cohen L (1991) Cometabolic biotransformation of trichloroethylene and chloroform by methanotrophs. PhD Dissertation, Stanford University, Palo Alto, CA

  2. Anthony C (1982) The biochemistry of methylotrophs. Academic Press, London

  3. Baker SC, Kelly DP, Murrell JC (1991) Microbial degradation of methanesulphonic acid, a missing link in the biogeochemical sulphur cycle. Nature 350:627–628

  4. Bedard C, Knowles R (1989) Physiology, biochemistry, and specific inhibitors of CH4, NH4 +, and CO oxidation by methanotrophs and nitrifiers. Microbiol Rev 53:68–84

  5. Berth P, Jeschke P (1989) Consumption and fields of application of LAS. Tenside Deterg 26:75–79

  6. Broholm K, Jensen BK, Christensen TH, Olsen L (1990) Appl Environ Microbiol 56:2488–2493

  7. Engel PC (1977) Enzyme Kinetics. John Wiley and Sons, NY pp 26–36

  8. Fogel MM, Taddeo AR, Fogel S (1986) Biodegradation of chlorinated ethenes by a methane-utilizing mixed culture. Appl Environ Microbiol 51:720–724

  9. Fox BG, Lipscomb JD (1990) Methane monooxygenase: A novel biological catalyst for hydrocarbon oxidation. In: Biological oxidation systems. Vol. 1, Hamilton G et al. (eds) Academic Press, Orlando, FL pp 367–388

  10. Giger W, Alder AC, Brunner PH, Marcomini A, Siegrist M (1989) Behaviour of LAS in sewage treatment and in sludge-treated soil. Tenside Deterg 26:95–100

  11. Haber CL, Allen LN, Zhas S, Hanson RS (1983) Methylotrophic bacteria: Biochemical diversity and genetics. Science 221:1147–1153

  12. Henry SM, Grbić-Galić D (1990) Effect of mineral media on trichloroethylene oxidation by aquifer methanotrophs. Microb Ecol 20:151–169

  13. Henry SM, Grbić-Galić D (1991a) Influence of endogenous and exogenous electron donors and trichloroethylene oxidation toxicity on trichloroethylene oxidation by methanotrophic cultures from a groundwater aquifer. Appl Environ Microbiol 57:236–244

  14. Henry SM, Grbić-Galić D (1991b) Inhibition of trichloroethylene oxidation by the transformation intermediate carbon monoxide. Appl Environ Microbiol 57:1770–1776

  15. Heyman JJ, Molof AH (1967) Initiation of biodegradation in surfactants. J Water Pollut Control Fed 39:50–62

  16. Higgins IJ, Best DJ, Hammond RC (1980) New findings in methane-utilizing bacteria highlight their importance in the biosphere and their commercial potential. Nature 286:561–564

  17. Higgins IJ, Best DJ, Hammond RC, Scott D (1981) Methane-oxidizing microorganisms. Microbiol Rev 45:556–590

  18. Hou CT (1984) Microbiology and biochemistry of methylotrophic bacteria. In: Hou CT (ed) Methylotrophs: microbiology, biochemistry and genetics. CRC Press Inc, Boca Raton, FL pp 1–53

  19. Hršak D, Bošnjak M, Johanides V (1981) Kinetics of linear alkylbenzene sulphonate and secondary alkane sulphonate biodegradation. Tenside Deterg 18:137–140

  20. Hršak D, Bošnjak M, Johanides V (1982) Enrichment of linear alkylbenzenesulphonate (LAS) degrading bacteria in continuous culture. J Appl Bacteriol 53:413–422

  21. Hršak D, Johanides V, Starčević M (1978) Dynamics of mixed bacterial population during linear alkylbenzenesulphonate biodegradation. In: Proc of 7th Int Congr on Surface Active Substances. Vneshtorgizdat Izd No. 7090 M, Moscow 4 pp 149–159

  22. Hršak D, Grbić-Galić D (1993) Biodegradation of linear alkylbenzenesulphonates (C11LAS) by mixed methanotrophic/heterotrophic and mixed heterotrophic bacterial cultures. Prehrambeno-tehnol Biotehnol Rev 31:7–14

  23. Hršak D (1994) Biodegradation of undecyl-benzenesulphonate by methane-oxidizing culture. (paper submitted for publication in Environmental Pollution)

  24. Huddleston RL, Allred RC (1963) Microbial oxidation of sulphonated alkylbenzenes. Develop Ind Microbiol 4:24–38

  25. Jiménez L, Breen A, Thomas N, Federle TW, Saylor GS (1991) Mineralization of linear alkylbenzene sulphonate by a four-member aerobic bacterial consortium. Appl Environ Microbiol 57:1566–1569

  26. Larson RJ (1990) Structure-activity relationships for biodegradation of linear alkylbenezensulphonates. Environ Sci Technol 24:1241–1246

  27. Mackay D, Stirn WY (1981) A critical review of Henry's law constants. J Phys Ref Data 10:1175–1199

  28. Marcomini A, Giger W (1988) Behaviour of LAS in sewage treatment. Tenside Deterg 25:226–229.

  29. McEvoy J, Giger W (1985) Accumulation of linear alkylbenzene surfactants in sewage sludges. Naturwissenschaften 72:429–431

  30. Schöberl P (1989) Basic principles of LAS biodegradation. Tenside Deterg 26:86–94

  31. Stirling DI, Dalton H (1979) The fortuitous oxidation and cometabolism of various carbon compounds by whole-cell suspensions of Methylococcus capsulatus (Bath.). FEMS Microbiol Letters 5:315–318

  32. Stirling DI, Dalton H (1981) Fortuitous oxidations by methane-utilizing bacteria. Nature 291:169–170

  33. Swisher RD (1987) Surfactant biodegradation, 2nd ed. Marcel Dekker Inc, NY pp 587–628

  34. Terzić S, Hršak D, Ahel M (1992a) Enrichment and isolation of linear alkylbenzenesulphonate (LAS) degrading bacteria from estuarine and coastal waters. Mar Pollut Bull 24:199–204

  35. Terzić S, Hršak D, Ahel M (1992b) Primary biodegradation kinetics of linear alkylbenzene sulphonates in estuarine waters. Wat Res 26(5):585–591

  36. Whittenbury R, Phillips KC, Wilkinson JF (1970) Enrichment, isolation and some properties of methane-utilizing bacteria. J Gen Microbiol 24:225–233

  37. Willetts AJ, Cain RB (1972) Microbial metabolism of alkylbenzene sulphonates. Biochem J 129:389–402

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Hršak, D. Aerobic transformation of linear alkylbenzenesulphonates by mixed methane-utilizing bacteria. Arch. Environ. Contam. Toxicol. 28, 265–272 (1995). https://doi.org/10.1007/BF00213101

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Keywords

  • Transformation Rate
  • Methanotrophs
  • Concentration Dependent Manner
  • High Transformation Rate
  • Preferential Capability