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Combined effects of tri-n-butyl tin (TBT) and diuron on marine periphyton communities detected as pollution-induced community tolerance


The effects of combined toxicity were studied, using marine periphyton communities exposed to mixtures of tri-n-butyl tin (TBT) and diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea, DCMU) in indoor aquaria during four weeks. The experimental design of the study followed a central composite design (CCD) and utilized dose-response surface methodology for evaluation of the results. The detection of pollution-induced community tolerance (PICT) was accomplished by short-term (1 h) tests on inhibition of photosynthesis. Both single-toxicant and two-toxicant short-term tests were used. Two tentative measures of tolerance are proposed to achieve convenient comparisons of the tolerances from the two-toxicant tests. With the detection of PICT, effects of the long-term exposure were recorded on diatom species richness, chlorophyll a accumulation and copepod abundance. The decrease of diatom species richness was accompanied by an increased tolerance (PICT), which was detectable by all tolerance measures used. Primary effects on microalgae were recorded as a decrease in chlorophyll a at higher toxicant concentrations. At lower concentrations, primary effects on copepods were found, which resulted in reduced grazing and increased chlorophyll a content.

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  1. Alabaster JS, Lloyd R (eds) (1982) Water quality criteria for freshwater fish, 2nd ed. Butterworths, London

  2. Aldridge WN, Street BW, Skilleter DN (1977) Halide-dependent and halide-independent effects of triorganotin and triorganolead compounds on mitochondrial functions. Biochem J 168:353–364

  3. Bayne CK, Rubin IB (1986) Practical experimental designs and optimization methods for chemists. VCH Publishers, NY, Weinheim

  4. Ben-Shlomo R, Nevo E (1988) Isozyme polymorphism as monitoring of marine environments: The interactive effect of cadmium and mercury pollution on the shrimp, Palaemon elegans. Mar Pollut Bull 19:314–317

  5. Blanck H (1984) Species dependent variation among organisms in their sensitivity to chemicals. Ecol Bull 36:107–119

  6. Blanck H, Wängberg SÅ (1988a) The validity of an ecotoxicological test system. Short-term and long-term effects of arsenate on marine periphyton communities in laboratory systems. Can J Fish Aquat Sci 45:1807–1815

  7. —, — (1988b) Induced community tolerance in marine periphyton established under arsenate stress. Can J Fish Aquat Sci 45:1816–1819

  8. --, -- (1991) Pattern of cotolerance in marine periphyton communities estabished under arsenate stress. Aquat Toxicol (in press)

  9. Blanck H, Wängberg SÅ, Molander S (1988) Pollution-induced community tolerance—a new ecotoxicological tool. In: Cairns J Jr, Pratt JR (eds) Functional testing of aquatic biota for estimating hazard of chemicals, ASTM STP 988. American Society for Testing and Materials, Philadelphia, pp 219–230

  10. Box GEP, Hunter WG, Hunter JS (1978) Statistics for experimenters, John Wiley & Sons, NY

  11. Brattsten LB, Holyoke CW, Jr., Leeper JR, Raffa KF (1986) Insecticide resistance: challenge to pest management and basic research. Science 231:1255–1260

  12. Cairns J Jr, Pratt JR (1989) The scientific basis of bioassays. Hydrobiologia 188/189:5–20

  13. Cairns J Jr, Niederlehner BR, Pratt JR (1990) Evaluation of joint toxicity of chlorine and ammonia to aquatic communities. Aquat Toxicol 16:87–100

  14. Clarke KR, Green RH (1988) Statistical design and analysis for a biological effects' study. Mar Ecol Prog Ser 46:213–226

  15. Dahl B, Blanck H (1990) Pollution-induced community tolerance (PICT) to tributyltin (TBT) in marine periphyton—field study and microcosm experiment. Proc 3rd Int Organotin Symp. Monaco April 17–20, 1990

  16. Deneer JW, Sinnige TL, Seinen W, Hermens JLM (1988) The joint acute toxicity to Daphnia magna of industrial organic chemicals at low concentrations. Aquat Toxicol 12:33–38

  17. Filbin GJ, Hough RA (1984) Extraction of 14C-labeled photosynthate from aquatic plants with dimethyl sulfoxide (DMSO). Limnol Oceanogr 29:426–428

  18. Granmo Å, Kollberg SO (1972) A new simple water flow system for accurate continuous flow tests. Water Res 6:1597–1599

  19. Hansson O, Wydrzynski T (1990) Current Perceptions of Photosystem-II. Photosynth Res 23:131–162

  20. Hermens J, Leeuwangh P (1982) Joint toxicity of mixtures of 8 and 24 chemicals to the guppy (Poecilia reticulata). Ecotoxicol Environ Safety 6:302–310

  21. Hiscox JD, Israelstam F (1978) A method for the extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57:1332–1334.

  22. Izawa S (1977) Inhibitors of electron transport. Encyclopedia of Plant Physiology V. Photosynthesis 1, pp 266–282

  23. Jeffrey SW, Humphrey GF (1975) New spectrophotometric equations for determining chlorophylls a, b, c 1 and c 2 in higher plants, algae and natural plankton. Biochim Physiol Pflanzen (BPP) 167:191–194

  24. Klerks PL, Levinton JS (1989) Effects of heavy metals in a polluted aquatic ecosystem. In: Levine SA, Harwell MA, Kelly JR, Kimball KD (eds) Ecotoxicology—Problems and Approaches, Springer-Verlag, NY, pp 41–67

  25. Könemann H (1981) Fish toxicity tests with mixtures of more than two chemicals: a proposal for a quantitative approach and experimental results. Toxicology 19:229–238

  26. Luoma SN (1977) Detection of trace contaminant effects in aquatic ecosystems. J Fish Res Board Can 34:436–439

  27. Molander S, Blanck H (1991) Detection of pollution-induced community tolerance (PICT) in marine periphyton communities established under diuron exposure. Aquat Toxicol (in press)

  28. Molander S, Blanck H, Söderström M (1990) Toxicity assessment by pollution-induced community tolerance (PICT), and identification of metabolites in periphyton communities after exposure to 4,5,6-trichloroguaiacol. Aquat Toxicol 18:115–136

  29. Okkerman PC, van der Plassche EJ, Sloof W, Van Leeuwen CJ, Canton JH (1991) Ecotoxicological effects assessment: A comparison of several extrapolation procedures. Ecotoxicol Environ Safety 21:182–193

  30. Pitelka LF (1988) Evolutionary response of plants to anthropogenic pollutants. Trends Ecol Evol 3:233–236

  31. Renger G (1986) Herbicide interaction with photosystem II: recent developments. Physiol Veg 24:509–521

  32. Shoaf WT, Lium BW (1976) Improved extraction of chlorophyll a and b from algae using dimethyl sulfoxide. Limnol Oceanog 21:926–928

  33. Sprague JB (1970) Measurement of pollutant toxicity to fish. II. Utilizing and applying bioassay results. Water Res 4:3–32

  34. Vouk VB, Butler GC, Upton AC, Parke DV, Asher SC (eds) (1987) Methods for assessing the effects of mixtures of chemicals, SCOPE 30, SGOMSEC 3, John Wiley & Sons, Chichester

  35. Wängberg SÅ, Heyman U, Blanck H (1991) Long-term and short-term arsenate toxicity to freshwater phytoplankton and periphyton in limnocorrals. Can J Fish Aquat Sci 48:173–182

  36. Watling-Payne AS, Selwyn MJ (1974) Inhibition and uncoupling of photophosphorylation in isolated chloroplasts by organotin, organomercury and diphenyleneiodonium compounds. Biochem J 142:65–74

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Molander, S., Dahl, B., Blanck, H. et al. Combined effects of tri-n-butyl tin (TBT) and diuron on marine periphyton communities detected as pollution-induced community tolerance. Arch. Environ. Contam. Toxicol. 22, 419–427 (1992). https://doi.org/10.1007/BF00212562

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  • Microalgae
  • Central Composite Design
  • Diuron
  • DCMU
  • Dimethylurea