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A role for the interferon response DNA sequence in directing transcription of the T18 d Tla gene

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Abstract

T18 d of BALB/c mice is a member of the Tla category of class I genes of the major histocompatibility complex of the mouse and is highly restricted in expression. Deletion analysis implies that an element essential to T18 d expression resides within the region −4 to +54. The homologous region of T3 d, a Tla gene which normally is not expressed in BALB/c mice, also has promoter activity. Thus the expressibility of T18 d vs T3 d is unlikely to be due to sequence differences in this region. A DNA-binding protein, factor VI, was found to bind to the region −33 to +54. DNase I footprinting analysis indicated that the DNA fragment 5'-ACTATAGTTTCACTTTTT-3' (+3 to +20) was protected by factor VI. This region includes the interferon response sequence (IRS). Homologous DNA segments of other class I genes, L d and D d, competed for factor VI in DNA-protein binding assay with lower affinity as compared with T18 d. In mutation analysis, the 3' portion of the IRS is more important than the 5' portion with respect to binding affinity of factor VI and to transcriptional activity in transfected cells. This result signifies a role of IRS in T18 d transcription and suggests that the mechanism of T18 d transcription might be unusual.

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Correspondence to King-Jen Chang.

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Address correspondence and offprint requests to: F.-W. Shen.

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Horie, M., Niikawa, J., Chang, K. et al. A role for the interferon response DNA sequence in directing transcription of the T18 d Tla gene. Immunogenetics 34, 293–298 (1991). https://doi.org/10.1007/BF00211993

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Keywords

  • Major Histocompatibility Complex
  • Transcriptional Activity
  • Binding Affinity
  • Promoter Activity
  • Mutation Analysis