Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Characterization of genomic polymorphism of an activation-associated antigen, Blast-1

  • 21 Accesses

  • 2 Citations


Blast-1 is a human activation-associated glycoprotein expressed on the surface of monocuclear cells, and a possible genetic marker for the manifestation of rheumatoid arthritis. In the present study, genomic polymorphism of the Blast-1 gene was analyzed using 100 healthy subjects. Restriction fragment length polymorphism (RFLP) of the Blast-1 gene was recognized only by Bam HI digestion among 46 restriction enzymes tested. The sizes of polymorphic fragments were 2.4 kilobase (kb) on the L band, and 1.9 kb on the S band. A family study demonstrated that the two alleles of the Blast-1 gene were inherited in a co-dominant Mendelian fashion. The genotype frequencies of homozygosity for the L and S bands were 47% and 42%, respectively, while the frequency of heterozygosity was 11%. The allele frequencies of the L and S bands were 0.68 and 0.32, respectively. The distribution of the Blast-1 genotypes in the present study was concordant with Hardy-Weinberg equilibrium (p>0.7), which indicates that the frequency of the Blast-1 gene in the population is derived from random mating in preceding generations. The results of the present study may provide useful information in disease associations with the Blast-1 gene.

This is a preview of subscription content, log in to check access.


  1. Ishihara, A., Hou, Y., and Jacobson, K.: The Thy-1 antigen exhibits rapid lateral diffusion in the plasma membrane of rodent lymphoid cells and fibroblasts. Proc Natl Acad Sci USA 84: 1290–1293, 1987

  2. Low, M. G., Ferguson, M. A., Futerman, A., and Silman, I.: Covalently attached phosphotidylinositol as a hydrophobic anchor for membrane proteins. Trends Biochem Sci 11: 212–215, 1986

  3. Southern, E. M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517, 1975

  4. Staunton, D. E. and Thorley-Lawson, D. A.: Molecular cloning of the lymphocyte activation marker Blast-1. EMBO J 6: 3695–3701, 1987

  5. Staunton, D. E., Fisher, R. C., LeBeau, M. M., Lawrence, J. B., Barton, D. E., Francke, U., Dustbin, M., and Thorley-Lawson, D. A.: BLAST-1 possesses a glycosyl-phosphatidylinositol (GPI) membrane anchor, is related to LEA-3 and OX-45, and maps to chromosome 1q21–23. J Exp Med 169: 1087–1099, 1989

  6. Stern, C.: The Hardy-Weinberg law. In C. Stern (ed.): Principles of Human Genetics, pp. 221–234, Freeman, San Francisco, 1973

  7. Thorley-Lawson, D. A., Schooley, R. T., Bhan, A. K., and Nadler, L. M.: Epstein-Barr virus superinduces a new human B cell differentition antigen (B-LAST 1) expressed on transformed lymphoblasts. Cell 30: 415–425, 1982

  8. Yeh, E., Reiser, H., Bamezai, A., and Rock, K. L.: TAP transcription and phosphotidylinositol linkage mutants are defective in activation through the T cell receptor. Cell 52: 665–674, 1988

Download references

Author information

Correspondence to Heihachiro Kashiwagi.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Matsui, Y., Shibano, K., Kashiwagi, H. et al. Characterization of genomic polymorphism of an activation-associated antigen, Blast-1. Immunogenetics 31, 188–190 (1990).

Download citation


  • Rheumatoid Arthritis
  • Arthritis
  • Healthy Subject
  • Allele Frequency
  • Restriction Enzyme