The transfer of plasmids was studied in a stirred fermentor in the course of mixed batch cultures combining recombinant strains of Lactococcus lactis subsp. lactis (donor strains) with L. lactis subsp. lactis CNRZ 268M3 (recipient strain). Donor strains contained one or two of the following plasmids (coding for erythromycin or chloramphenicol resistance): pIL205 (self-transmissible), pIL252, pIL253 (non-transmissible but mobilizable by pIL205, respectively small and large copy number) and pE194 (inserted in the chromosome). Only self-transmissible plasmid pIL205 was transferred, with frequencies ranging from 10−7 to 10−8 after 12 h of fermentation. These frequencies were 60–400 times lower than in unstirred M17 broth and 100 000 times lower than on agar medium. In the latter case, non-transmissible plasmids pIL252 and pIL253 were mobilized by pIL205 with a frequency of about 10−5–10−6.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Anderson DG, McKay LL (1983) Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol 46:549–552
Chopin A, Chopin MC, Moillo-Batt A, Langella P (1984) Two plasmid-determined restriction and modification systems in Streptococcus lactis. Plasmid 11:260–263
El Alami N, Boquien CY, Corrieu G (1992) Batch cultures of recombinant Lactococcus lactis subsp. lactis in a stirred fermentor. I. Effect of plasmid content on bacterial growth and on genetic stability in pure cultures. Appl Microbiol Biotechnol
Gabin-Gauthier K, Gratadoux JJ, Richard J (1991) Conjugal plasmid transfer between lactococci on solid surface matings and during cheese making. FEMS Microbiol Ecol 85:133–140
Gasson MJ, Davies FL (1980) Conjugal transfer of the drug resistance plasmid pAM\1 in the lactic streptococci. FEMS Microbiol Lett 7:51–53
Huggins AR, Sandine WE (1984) Differentiation of fast and slow milk-coagulating isolates in strains of lactic streptococci. J Dairy Sci 67:1674–1679
Hull RR (1977) Methods for monitoring bacteriophages in cheese factories. Aust J Dairy Technol 32:63–64
McKay LL, Baldwin KA, Walsh PM (1980) Conjugal transfer of genetic information in group N streptococci. Appl Environ Microbiol 40:84–91
Neve H, Geis A, Teuber M (1987) Conjugation, a common plasmid transfer mechanism in lactic acid streptococci of dairy starter cultures. Syst Appl Microbiol 9:151–157
Ramos P, Novel M, Lemosquet M, Novel G (1983) Fragmentation du plasmide lactose-protéase chez des dérivés lactose-négatifs de Streptococcus lactis et de Streptococcus lactis ssp. diacetylactis. Ann Microbiol 134b:387–399
Snook RJ, McKay LL (1981) Conjugal transfer of lactose-fermenting ability among Streptococcus cremoris and Streptococcus lactis strains. Appl Environ Microbiol 42:904–911
Steele JJ, McKay LL (1989) Conjugal transfer of genetic material in lactococci: a review. J Dairy Sci 72:3388–3397
Yao PX, Ohtake H, Toda K (1990) Factors affecting conjugative transfer rates of plasmids in batch and continuous cultures. J Ferment Bioeng 69:215–219
Correspondence to: C.-Y. Boquien
About this article
Cite this article
El Alami, N., Boquien, C. & Corrieu, G. Batch cultures of recombinant Lactococcus lactis subsp. lactis in a stirred fermentor. Appl Microbiol Biotechnol 37, 364–368 (1992). https://doi.org/10.1007/BF00210993
- Agar Medium