Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Batch cultures of recombinant Lactococcus lactis subsp. lactis in a stirred fermentor

II. Plasmid transfer in mixed cultures

  • 34 Accesses

  • 2 Citations

Summary

The transfer of plasmids was studied in a stirred fermentor in the course of mixed batch cultures combining recombinant strains of Lactococcus lactis subsp. lactis (donor strains) with L. lactis subsp. lactis CNRZ 268M3 (recipient strain). Donor strains contained one or two of the following plasmids (coding for erythromycin or chloramphenicol resistance): pIL205 (self-transmissible), pIL252, pIL253 (non-transmissible but mobilizable by pIL205, respectively small and large copy number) and pE194 (inserted in the chromosome). Only self-transmissible plasmid pIL205 was transferred, with frequencies ranging from 10−7 to 10−8 after 12 h of fermentation. These frequencies were 60–400 times lower than in unstirred M17 broth and 100 000 times lower than on agar medium. In the latter case, non-transmissible plasmids pIL252 and pIL253 were mobilized by pIL205 with a frequency of about 10−5–10−6.

This is a preview of subscription content, log in to check access.

References

  1. Anderson DG, McKay LL (1983) Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol 46:549–552

  2. Chopin A, Chopin MC, Moillo-Batt A, Langella P (1984) Two plasmid-determined restriction and modification systems in Streptococcus lactis. Plasmid 11:260–263

  3. El Alami N, Boquien CY, Corrieu G (1992) Batch cultures of recombinant Lactococcus lactis subsp. lactis in a stirred fermentor. I. Effect of plasmid content on bacterial growth and on genetic stability in pure cultures. Appl Microbiol Biotechnol

  4. Gabin-Gauthier K, Gratadoux JJ, Richard J (1991) Conjugal plasmid transfer between lactococci on solid surface matings and during cheese making. FEMS Microbiol Ecol 85:133–140

  5. Gasson MJ, Davies FL (1980) Conjugal transfer of the drug resistance plasmid pAM\1 in the lactic streptococci. FEMS Microbiol Lett 7:51–53

  6. Huggins AR, Sandine WE (1984) Differentiation of fast and slow milk-coagulating isolates in strains of lactic streptococci. J Dairy Sci 67:1674–1679

  7. Hull RR (1977) Methods for monitoring bacteriophages in cheese factories. Aust J Dairy Technol 32:63–64

  8. McKay LL, Baldwin KA, Walsh PM (1980) Conjugal transfer of genetic information in group N streptococci. Appl Environ Microbiol 40:84–91

  9. Neve H, Geis A, Teuber M (1987) Conjugation, a common plasmid transfer mechanism in lactic acid streptococci of dairy starter cultures. Syst Appl Microbiol 9:151–157

  10. Ramos P, Novel M, Lemosquet M, Novel G (1983) Fragmentation du plasmide lactose-protéase chez des dérivés lactose-négatifs de Streptococcus lactis et de Streptococcus lactis ssp. diacetylactis. Ann Microbiol 134b:387–399

  11. Snook RJ, McKay LL (1981) Conjugal transfer of lactose-fermenting ability among Streptococcus cremoris and Streptococcus lactis strains. Appl Environ Microbiol 42:904–911

  12. Steele JJ, McKay LL (1989) Conjugal transfer of genetic material in lactococci: a review. J Dairy Sci 72:3388–3397

  13. Yao PX, Ohtake H, Toda K (1990) Factors affecting conjugative transfer rates of plasmids in batch and continuous cultures. J Ferment Bioeng 69:215–219

Download references

Author information

Additional information

Correspondence to: C.-Y. Boquien

Rights and permissions

Reprints and Permissions

About this article

Cite this article

El Alami, N., Boquien, C. & Corrieu, G. Batch cultures of recombinant Lactococcus lactis subsp. lactis in a stirred fermentor. Appl Microbiol Biotechnol 37, 364–368 (1992). https://doi.org/10.1007/BF00210993

Download citation

Keywords

  • Fermentation
  • Erythromycin
  • Lactis
  • Chloramphenicol
  • Agar Medium