Electron microscopy of the rat lymph node disclosed reticular cells in close association with bundles of collagenous fibrils 30–45 nm in diameter, and electron lucent “amorphous” substances surrounded by microfibrils 11 nm in diameter, with junctional complexes. The amorphous substances, distinctly revealed by orcein staining, and the microfibrils showed morphological characteristics of elastic fibers. Since elastase digestion of 2% glutaraldehyde fixed specimens induced the selective disappearance of the orcein-stainable substances, it was concluded that they are elastin. Elastin often had a close contact with the plasma membrane of reticular cells. It is suggested that reticular cells synthesize elastic fibers.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Bloom W, Fawcett DW (1975) In: Textbook of Histology, 10th ed. WB Saunders Company, Philadelphia, p 165
Clark LS (1962) The reticulum of lymph nodes in mice studied with the electron microscope. Am J Anat 110:217–257
Greenlee TK Jr, Ross R, Hartman JL (1966) The fine structure of elastic fibers. J Cell Biol 30:59–71
Ishii T (1966) Über den lichtmikroskopischen Feinbau der Retikulumfasern des Lymphknotens (ein Beitrag zur Frage von Natur und Aufbau der Retikulumfasern). Proc Jap Soc Reticuloendothelial System 6:24–37
Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol 27:137A-138A
Leak LV (1974) Freeze-fracture, transmission and scanning electron microscopic studies on the normal lymph node. Anat Rec 178:399–400
Lillie DR, Fullmer HM (1976) In: Histopathologic technic and practical histochemistry, 4th ed. McGraw-Hill Book Company, New York, p 302–303
Luck SC, Nopajaroonsri C, Simon GT (1973) The architecture of the normal lymph node and hemolymph node: A scanning and transmission electron microscopic study. Lab Invest 29:258–264
Mori Y, Lennert K (1969) In: Electron microscopic atlas of lymph node cytology and pathology, Kuchemann K translat. Springer-Verlag, Berlin Heidelberg New York, p 10
Nakamura H, Kanai C, Mizuhira V (1977) An electron stain for elastic fibers using orcein. J Histochem Cytochem 25:306–308
Rhodin JAD (1974) In: Histology, a text and atlas. Oxford University Press, New York, p 384–385
Ross R (1971) The smooth muscle cell II: Growth of smooth muscle in culture and formation of elastic fibers. J Cell Biol 50:173–186
Ross R, Klebanoff SJ (1971) The smooth muscle cell I: In vivo synthesis of connective tissue proteins. J Cell Biol 50:159–171
Sörenson GD (1960) An electron microscopic study of popliteal lymph nodes from rabbits. Am J Anat 107:73–96
About this article
Cite this article
Miyata, K., Takaya, K. Elastic fibers associated with collagenous fibrils surrounded by reticular cells in lymph nodes of the rat as revealed by electron microscopy after orcein staining. Cell Tissue Res. 220, 445–448 (1981). https://doi.org/10.1007/BF00210522
- Lymph node
- Reticular cell
- Reticular fiber
- Elastic fiber